Blasticidin S

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Blasticidin S
Blasticidin S.svg
Names
IUPAC name
4-amino-1-[4-({(3S)-3-amino-5-[[amino(imino)methyl](methyl)amino]pentanoyl}amino)-2,3,4-trideoxy-β-D-erythro-hex-2-enopyranuronosyl]pyrimidin-2(1H)-one
Identifiers
2079-00-7 YesY
ChEBI CHEBI:15353 N
ChEMBL ChEMBL476894 YesY
ChemSpider 148673 YesY
Jmol-3D images Image
KEGG C02010 YesY
PubChem 258
Properties
C17H26N8O5
Molar mass 422.44 g/mol
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
 N verify (what isYesY/N?)
Infobox references

Blasticidin S is an antibiotic that is produced by Streptomyces griseochromogenes. In biological research, specifically genetic engineering, it is used to select transformed cells which have been engineered to carry a resistance gene for blasticidin. In short, DNA of interest is fused to DNA encoding a resistance gene, and then is transformed into cells. After allowing time for recovery and for cells to begin transcribing and translating their new DNA, blasticidin is added. Now only the cells that have the new DNA can grow.

Resistance genes[edit]

Three resistance genes have been cloned:

  • bls (an acetyltransferase) from Streptoverticillum sp. which itself produces blasticidin in a natural example of biological warfare
  • bsr (a blasticidin-S deaminase) from Bacillus cereus (other bsr genes are known as well, see listings in Genbank)
  • BSD (another deaminase) from Aspergillus terreus

bsr and BSD are the most commonly used resistance genes. The proteins produced from these genes enable the cells carrying them to produce protein in the presence of blasticidin.

Mechanism of action[edit]

Blasticidin prevents the growth of both eukaryotic and prokaryotic cells. It works by inhibiting termination step of translation and peptide bond formation (to lesser extent) by the ribosome. This means that cells can no longer produce new proteins through translation of mRNA.

References[edit]