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CrysTBox
Developer(s)Miloslav Klinger
Initial release9 December 2014; 9 years ago (2014-12-09)
Written inMATLAB
Operating systemMicrosoft Windows
Available inEnglish
TypeScientific
LicenseFree for non-commercial use
Websitecrystbox.fzu.cz

CrysTBox (Crystallographic Tool Box) is a suite of computer tools designed to accelerate material research based on transmission electron microscope images via highly accurate automated analysis and interactive visualization. Relying on artificial intelligence and computer vision, CrysTBox makes routine crystallographic analyses simpler, faster and more accurate compared to human evaluators. The high level of automation together with sub-pixel precision[1] and interactive visualization makes the quantitative crystallographic analysis accessible even for non-crystallographers allowing for an interdisciplinary research. Simultaneously, experienced material scientists can take advantage of advanced functionalities for comprehensive analyses.

CrysTBox is being developed in the Laboratory of electron microscopy at the Institute of Physics of the Czech Academy of Sciences. For academic purposes, it is available for free. As of 2022, the suite has been deployed at research and educational facilities in more than 90 countries[2] supporting research of ETH Zurich,[3] Lawrence Berkeley National Laboratory,[4] Max Planck Institutes,[5] Chinese Academy of Sciences,[6] Fraunhofer Institutes[7] or Oxford University.[8]

Suite

As a scientific tool, CrysTBox suite is freely available for academic purposes, it supports file formats widely used in the community and offers interconnection with other scientific software.

Availability

CrysTBox is freely available on demand for non-commercial use by non-commercial subjects. The only safe way to download CrysTBox installers is via a request form on the official website.[note 1] Commercial use is not allowed due to the license of MATLAB used for CrysTBox compilation.[2]

Notable research and users

Besides education, CrysTBox is mainly used in research with fields of application spanning from nuclear research[9] to archaeology and paleontology.[10] Among others, the suite was employed in development of additive manufacturing (including 3D printed biodegradable alloys,[7] metallic glass[11] or high-entropy alloys[12]), resistant coatings,[13] laser shock peening,[14] water cleaning technologies[15] or characterization of 50 million years old flint.[10]

Institutions whose research was supported by CrysTBox include educational facilities such as ETH Zurich,[3] University of California,[16] Uppsala University,[13] Oxford University,[8] University of Waterloo,[15] Indian Institute of Technology,[17] Nanyang Technological University[12] or University of Tokyo[18] as well as research institutes like Max Planck Institutes,[12] Chinese Academy of Sciences,[6] Fraunhofer Institutes[7] or US national laboratories (NL) such as Oak Ridge NL,[9] Lawrence Berkeley NL,[4] Idaho NL[9] and Lawrence Livermoore NL.[14]

Limitations and disadvantages

CrysTBox is compiled to a stand-alone installers using MATLAB Compiler. Therefore, 1-2 GB of MATLAB libraries are installed together with the toolbox.

The diffraction simulation used in cellViewer is based on kinematic diffraction theory. This allows for a real-time response to user interaction, but it does not cover advanced diffraction features like double diffraction covered by dynamical diffraction theory, even though some phenomena caused by multiple electron-matter interactions are visualized by CrysTBox - for instance Kikuchi lines.

The analytical tools provide correction for scale calibration imperfections, but does not provide adjustment for image distortions such as elliptical distortion. If high-accuracy measurement is needed or if the distortion exceeds standard levels, appropriate tools[19] should be applied prior to the analysis.

Crystallographic visualization tools

In order to visualize functional relations and provide better understanding of experimental data, the graphical interface emphasize user interactivity and functional interconnection. There are two visualization tools in the suite: one depicting single material while another being focused on intergrowths of two different materials.

cellViewer - single crystal visualization

User interface of cellViewer.

CellViewer allows to visualize the sample material in four modes widely used in material research:

Graphical user interface provides user with two interactive views side by side. These views can display arbitrary combination of the four aforementioned visualization modes allowing to perceive their mutual relations. For instance, rotation of the atomic structure in direct space leads (if set so) to an instant update of the simulated diffraction pattern. If any diffraction spot is selected, corresponding crystallographic planes are shown in the unit cell etc. Such interconnections are implemented for each pair of the four available visualization modes. The electronic visualization allows to simplify understanding of widely used, yet less intuitive representations such as the inverse pole figure. For instance by drawing the coloured triangle of the inverse pole figure into the stereographic projection or to the more intuitive 3D atomic structure.[1]

  • Examples of various usage of visualization modes with magnesium as sample material
  • 3D atomic structure with selected planes
    3D atomic structure with selected planes
  • Diffraction pattern with selected reflections (planes)
    Diffraction pattern with selected reflections (planes)
  • 3D atomic structure with inverse pole figure
    3D atomic structure with inverse pole figure
  • Stereographic projection with inverse pole figure
    Stereographic projection with inverse pole figure
  • Inverse pole figure
    Inverse pole figure

ifaceViewer - intergrowth visualization

User interface of ifaceViewer.

The ifaceViewer allows for visualization of two misoriented materials and their interface such as crystal twins or grain boundaries. The user interface provides three views: two smaller views, each depicting one unit cell of selected material and orientation, and a larger view depicting an appropriate interface of the two structures. The interface can be visualized in four modes:

All three views in the user interface are functionally interconnected. If the content of one view is rotated by the user, the other views follow. If a crystallographic plane or direction is selected in one view, it is shown in other views and corresponding crystallographic indices are stated. The tool also allows to highlight coincident site lattice or calculate the list of planes and directions which are parallel or nearly parallel in the two misoriented materials.[1]

  • Interface of two aluminum lattices in Σ5 misorientation shown in four available visualization modes of CrysTBox IfaceViewer
  • 3D model of both unit cells
    3D model of both unit cells
  • Wire model of both unit cells
    Wire model of both unit cells
  • Interface cross section
    Interface cross section
  • Bulk representation
    Bulk representation

Automated analysis of TEM images

CrysTBox offers tools for automated processing of diffraction patterns and high-resolution transmission electron microscope images. Since the tools employ algorithms of artificial intelligence and computer vision, they are designed to require minimal operator effort providing higher accuracy compared to manual evaluation. Four analytical tools can be used to index diffraction patterns, measure lattice constants (distances and angles), sample thickness etc. Despite the high level of automation, the user is able to control the whole process and perform individual steps manually if needed.

diffractGUI - HRTEM and diffraction processing

User interface of diffractGUI.

DiffractGUI allows for an automated analysis of diffraction patterns and high-resolution images of single crystal or limited number of crystallites. It is able to determine crystal orientation, index individual diffraction spots and measure interplanar angles and distances in picometric precision.[20] The input image may depict:

The input image is processed in the following steps:

  1. Preprocessing with accordance to the settings and image nature (resolution and noise reduction, Fourier transform for direct space images etc.).
  2. Detection of diffraction reflections at various scales[21] (difference of Gaussians typically used for spot detection, Hough transform[22][23] for CBED disk detection[24]).
  3. The strongest detections are selected across the scale space.
  4. A regular lattice is fit to the set of the strongest detections using RANSAC[25] algorithm.
  5. Lengths and angles of the lattice basis vectors are measured.
  6. Crystal lattice orientation is determined and diffraction reflections are identified using theoretical parameters of the sample material. [26][27]

Compared to human evaluation, diffractGUI considers tens or even hundreds of diffraction spots at once and, therefore, can localize the pattern with sub-pixel precision.[20]

  • Individual steps of automated procedure illustrated on diffraction pattern of titanium
  • Input image
    Input image
  • 30 strongest reflections detected
    30 strongest reflections detected
  • Regular lattice
    Regular lattice
  • Lattice basis and parameters measured
    Lattice basis and parameters measured
  • Reflections identified for zone axis [0 -1 1]
    Reflections identified for zone axis [0 -1 1]

ringGUI - ring diffraction analysis

User interface of ringGUI.

RingGUI allows for an automated processing of ring diffraction images of polycrystalline or powder samples. It can be used to identify the diffraction rings, quantify the interplanar distances and thus characterize or identify the sample material. With known material, it can assist in microscope calibration. The input image is processed as follows:

  1. beam-stopper detection,
  2. localization of the ring center,
  3. quantification of the diffraction profile and estimation of its background intensity,
  4. identification of the rings[27] in the image (peaks in the profile).

The results can be further processed and visualized in two interactive, functionally interconnected graphical elements:

  • Interactive diffraction image – allows the user to improve readability of the diffraction image by removing the beam-stopper, subtracting the background, revealing faint or spotty rings or by crystallographic identification of the depicted rings.
  • Diffraction profile – circular average of the image intensities depicts the peaks corresponding to the rings and their match with theoretical values known for given sample material.

Both, the diffraction image as well as diffraction profile can be used to select diffraction rings with a mouse click. The corresponding ring is then highlighted in both graphical representations and details are listed.[1]

  • Examples of patrtial and final results presented via interactive diffraction image of MgO
  • Input image
    Input image
  • Beam-stopper detected
    Beam-stopper detected
  • Ring centre localized
    Ring centre localized
  • Background removed
    Background removed
  • Beam-stopper removed
    Beam-stopper removed
  • Background and beam-stopper removed with faint rings revealed
    Background and beam-stopper removed with faint rings revealed
  • Rings identified
    Rings identified
  • Comparison of experimental and theoretical rings
    Comparison of experimental and theoretical rings

twoBeamGUI - sample thickness estimation

User interface of twoBeamGUI.

Sample thickness can be estimated using twoBeamGUI from a convergent beam electron diffraction pattern (CBED) in two beam approximation.[26][28] The procedure is based on an automated extraction of the intensity profile across the diffracted disk in the following steps:

  1. diffraction disk radius is determined using multi-scale Hough transform,[24]
  2. the transmitted and diffracted disks are localized and the reflection is indexed,[26][27]
  3. the disks are horizontally aligned, cropped out and profiles are measured across the disks,
  4. the profile across the diffracted disk is matched with a series of profiles automatically simulated[26] for given material, reflection and specified thickness range.

Once the procedure is completed, the measured profile and the most similar simulated profile are displayed with the diffracted disk on the background. This allows the user to verify correctness of the automated estimate and easily check the similarity of other intensity profiles within the specified thickness range.[1]

  • Individual steps of automated thickness estimation of titanium sample
  • Input image
    Input image
  • Disks detected
    Disks detected
  • Disks aligned with profiles measured
    Disks aligned with profiles measured
  • Comparison of measured (blue) and simulated (green) profile for thickness of 139 nm
    Comparison of measured (blue) and simulated (green) profile for thickness of 139 nm

gpaGUI - geometric phase analysis

User interface of gpaGUI.

The tool called gpaGUI provides an interactive interface for geometric phase analysis. It allows to generate 2D maps of various crystallographic quantities using high-resolution images.[29][30]

Since the geometric phase analysis is performed in frequency domain, the high-resolution image needs to be transformed into frequential representation using Fourier transform. Mathematically, the frequential image is a complex matrix with the size equal to the original image. Crystallographically, it can be seen as an artificial diffraction pattern of the original image depicting intensity peaks corresponding to the crystallographic planes present in the original image. After performing desired calculations, the frequential representation can be transformed back to the original spatial domain using inverse Fourier transform.

Various crystallographic analyses can be performed using the frequential image. If it is filtered so that only the information from a region close to a particular diffraction spot is used (the rest is set to zero), a filtered direct image obtained by inverse Fourier transform then depicts only the planes corresponding to the selected diffraction spot. Moreover, due to its complex nature, the frequential image can be used to calculate amplitude and phase. Together with a vector of one crystallographic plane depicted in the image, they can be used to generate a 2D map interplanar distance of given plane.[29] If two vectors of non-parallel planes are known, the method can be used to generate maps of strain and displacement.[30]

Graphical user interface of gpaGUI is vertically divided into two halves, each of which contains:

  • Diffractogram preview allowing to select one diffraction spot corresponding to a crystallographic plane.
  • Visualization of a selected quantity (input image, filtered image or one of the maps mentioned above) allowing to select point of interest or region of interest for further analysis.
  • Results of detailed analysis of point or region of interest. The point analysis allows the user to select any pixel of the visualized map to see exact values of the particular pixel and its closest neighbourhood. If analysis of broader area is needed, a polygonal region can be outlined in the map allowing to enumerate its statistical details: mean, standard deviation, median, minimum, maximum and total area of the polygon.

Since each half of the interface allows to specify one crystallographic plane, gpaGUI allows to calculate all the aforementioned crystallographic quantities including those which require two vectors. Precision and repeatability of the whole analysis relies on accuracy of the diffraction peak localization. To overcome inaccuracy of manual peak localization (with a mouse click), gpaGUI provides a possibility to process the input image with diffractGUI in order to accurately localize and index the peaks.[1]

  • Examples of 2D maps generated using gpaGUI from high resolution image of magnesium.
  • Input image
    Input image
  • Filtered image of plane (0 1 0)
    Filtered image of plane (0 1 0)
  • Map of (0 1 0) d-spacing
    Map of (0 1 0) d-spacing
  • Map of XX component of strain tensor
    Map of XX component of strain tensor
  • Map of X component of displacement vector
    Map of X component of displacement vector

See also

Notes

  1. ^ CrysTBox is not distributed by any website aggregating various software installers or uninstallers. Installers downloaded from these sources can contain malware. Similarly, no additional software is required to uninstall CrysTBox. Any software pretending to do so has no connection to CrysTBox developer.

References

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  2. ^ a b "CrysTBox - Crystallographic Toolbox". Fyzikální ústav Akademie věd České republiky. 2021-11-16.
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