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E100 or E-100 may refer to:
- Panzerkampfwagen E-100, a proposed German World War II super-heavy tank
- Saab 9-5 E100, a car model
- Toyota Corolla (E100), a car
- Neat ethanol fuel (fuel blend with almost 100% ethanol)
- Curcumin, food coloring (from turmeric) designated by E number E100
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