Flow injection analysis

From Wikipedia, the free encyclopedia
Jump to: navigation, search

Flow injection analysis (FIA) is an approach to chemical analysis that is accomplished by injecting a plug of sample into a flowing carrier stream.[1][2][3] The principle is similar to that of segmented flow analysis (SFA) but no air is injected into the sample or reagent streams.


FIA is an automated method of chemical analysis in which a sample is injected into a flowing carrier solution that mixes with reagents before reaching a detector. Over past 30 years FIA techniques developed into a wide array of applications using spectrophotometric, fluorescence, atomic absorption, mass spectrometry and other methods of instrumental analysis for detection. Based on computer control, Flow Injection evolved into Sequential Injection and Bead Injection which are novel techniques based on flow programming. FIA literature comprises over 22000 scientific papers and 22 monographs. A comprehensive review of FIA methodology is available in 2015 Edition of FIA Tutorial. www.flowinjectiontutorial.com, where in Chapter 2 the most recent developments are highligted

Principles of Operation[edit]

A sample (analyte) is injected into a carrier solution which mixes through radial and convection diffusion with a reagent for a period of time (depends on the flow rate and the coil length and diameter) before the sample passes through a detector to waste. A Peristaltic pump is the most commonly used pump in FIA instruments but new variants of FIA technique use computer controlled syringe pumps that generate discontinuous flow that is precisely choreographed to the needs of assay protocol. The result is dramatic decrease of sample and reagent consumption and waste generation. The new technologies broadened applicability of FIA technique from laboratory serial assays to research applications and continuous monitoring of environmental and industrial processes.[4]


A flow through detector is located downstream from the sample injector and records a chemical physical parameter. Various different types of detector can be used[5] For example:


An experiment that is used in analytical chemistry lab courses to familiarize students with FIA is the determination of phosphate by flow injection analysis. The experiment involves calibrating an FIA system, optimizing the system for detection of phosphate and finding the amount of phosphate in an unknown sample.

See also[edit]


  1. ^ Xu, Weihong; Sandford, Richard; Worsfold, Paul; Carlton, Alexandra; Hanrahan, Grady (2005). "Flow Injection Techniques in Aquatic Environmental Analysis: Recent Applications and Technological Advances". Critical Reviews in Analytical Chemistry 35 (3): 237. doi:10.1080/10408340500323362. 
  2. ^ Tyson, Julian F. (1985). "Flow injection analysis techniques for atomic-absorption spectrometry. a review". The Analyst 110 (5): 419–569. Bibcode:1985Ana...110..419T. doi:10.1039/an9851000419. PMID 4025835. 
  3. ^ Anastos, N.; Barnett, NW; Hindson, BJ; Lenehan, CE; Lewis, SW (2004). "Comparison of soluble manganese(IV) and acidic potassium permanganate chemiluminescence detection using flow injection and sequential injection analysis for the determination of ascorbic acid in Vitamin C tablets". Talanta 64 (1): 130–4. doi:10.1016/j.talanta.2004.01.021. PMID 18969577. 
  4. ^ Ruzicka, Jarda. "Flow Injection Analysis". Retrieved 25 August 2013. 
  5. ^ Trojanowicz, M. (2000). Flow injection analysis : instrumentation and applications. World Scientific. 

5. J. Ruzicka Flow Injection Analysis. Tutorial 2015 Edition www.flowinjectiontutorial.com

Further reading[edit]

  • Trojanowicz, Marek (2000). Flow injection analysis: instrumentation and applications. Singapore: World Scientific. ISBN 981-02-2710-8. 
  • Hansen, Elo Harald; Růžička, Jaromír (1988). Flow injection analysis. New York: Wiley. ISBN 0-471-81355-9. 
  • Martínez Calatayud, José (1996). Flow injection analysis of pharmaceuticals: automation in the laboratory. Washington, DC: Taylor & Francis. ISBN 0-7484-0445-7. 
  • Pacey, Gil E.; Karlberg, Bo (1989). Flow injection analysis: a practical guide. Amsterdam: Elsevier. ISBN 0-444-88014-3. 

External links[edit]