GabT RNA motif

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gabT RNA motif
GabT-RNA.svg
Consensus secondary structure of gabT RNAs
Identifiers
Symbol gabT RNA
Rfam RF01738
Other data
RNA type Cis-regulatory element
Domain(s) Pseudomonas

The gabT RNA motif is the name of a conserved RNA structure identified by bioinformatics whose function is unknown.[1] The gabT motif has been detected exclusively in bacteria within the genus Pseudomonas, and is found only upstream of gabT genes, and downstream to gabD genes.

Although it is agreed that the gabT and gabD genes encode a transaminase and dehydrogenase, respectively, it is less clear what substrates they operate on. Evidence suggests that they catalyze the transamination of GABA and the dehydrogenation of the product to form succinate, an intermediate in the Krebs cycle.[2] But it was also shown that they can produce glutarate from delta-aminovalerate (via transamination and dehydrogenation reactions) as part of the degradation of lysine.[3][4] Both the gabD and gabT genes were shown, in various experiments, to be induced by metabolites such as agmatine and lysine that would fit with either of the two activities just mentioned for the products of gabD/gabT.[2][3][5] The gabT RNA is probably not participating in this mode of regulation, unless it can regulate the upstream gene.[1]

The predicted Shine-Dalgarno sequence (ribosome-binding site) is contained within the stem-loop called "P2" in some gabT RNAs, and nearby to the "P1" stem-loop in those gabT RNAs that do not use a P2 stem-loop. This proximity hints at a mechanism for gene regulation that gabT RNAs might effect.

References[edit]

  1. ^ a b Weinberg Z, Wang JX, Bogue J, et al. (March 2010). "Comparative genomics reveals 104 candidate structured RNAs from bacteria, archaea and their metagenomes". Genome Biol. 11 (3): R31. PMC 2864571Freely accessible. PMID 20230605. doi:10.1186/gb-2010-11-3-r31. 
  2. ^ a b Chou HT, Kwon DH, Hegazy M, Lu CD (March 2008). "Transcriptome analysis of agmatine and putrescine catabolism in Pseudomonas aeruginosa PAO1". J. Bacteriol. 190 (6): 1966–75. PMC 2258879Freely accessible. PMID 18192388. doi:10.1128/JB.01804-07. 
  3. ^ a b Ochsner UA, Wilderman PJ, Vasil AI, Vasil ML (September 2002). "GeneChip expression analysis of the iron starvation response in Pseudomonas aeruginosa: identification of novel pyoverdine biosynthesis genes". Mol. Microbiol. 45 (5): 1277–87. PMID 12207696. doi:10.1046/j.1365-2958.2002.03084.x. 
  4. ^ Yamanishi Y, Mihara H, Osaki M, et al. (May 2007). "Prediction of missing enzyme genes in a bacterial metabolic network. Reconstruction of the lysine-degradation pathway of Pseudomonas aeruginosa". FEBS J. 274 (9): 2262–73. PMID 17388807. doi:10.1111/j.1742-4658.2007.05763.x. 
  5. ^ Espinosa-Urgel M, Ramos JL (November 2001). "Expression of a Pseudomonas putida aminotransferase involved in lysine catabolism is induced in the rhizosphere". Appl. Environ. Microbiol. 67 (11): 5219–24. PMC 93293Freely accessible. PMID 11679348. doi:10.1128/AEM.67.11.5219-5224.2001. 

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