Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation.
Hydrophilic recombinant gp100 protein (HR-gp100) has been topically applied on human intact skin in vitro, and used as a vaccine in a mouse model. It was demonstrated that HR-gp100 permeates into human skin, and is processed and presented by human dendritic cells. In the mouse model, an HR-gp100-based vaccine triggered antigen-specific T cell responses, as shown by proliferation assays, ELISA and intracellular staining for IFN-γ.
- Eisenberg, G; Machlenkin, A; Frankenburg, S; Mansura, A; Pitcovski, J; Yefenof, E; Peretz, T; Lotem, M.Sharett (2010). "Transcutaneous immunization with hydrophilic recombinant gp100 protein induces antigen-specific cellular immune response". Cell Immunol. 266 (1): 98–103. doi:10.1016/j.cellimm.2010.09.003.
|This biology article is a stub. You can help Wikipedia by expanding it.|