Hunky is a mother burro and Spunky is her young son. This story takes place in the old west, where a prospector attempts to make Spunky into his pack animal. Hunky and Spunky was nominated for the 1938 Academy Award for Best Short Subject (Cartoons). Fleischer Studios went on to produce six more cartoons featuring Hunky and Spunky: Always Kickin' (1939), The Barnyard Brat (1939), A Kick in Time (1940), Snubbed by a Snob (1940), You Can't Shoe a Horsefly (1940), and Vitamin Hay (1941). The series ended in 1941 with Vitamin Hay.
A positive contemporary review of Hunky and Spunky in Film Daily praised the short for introducing "funny new characters," and stated that the short's device of having the animals speak in "donkey talk" "will amuse the kids" 
Hunky is teaching her son Spunky how to kick properly. She has him practice with a mattress propped against a tree. Spunky befriends a family of birds who are building a nest. Spunky copies their design and builds a nest of his own. Although he is a donkey, he tries to imitate the birds in flight and falls out of his nest. A vulture kidnaps the baby bird from the nest and Spunky follows them. Spunky uses his newfound kicking abilities to send the vulture packing and return the baby bird to its parents.
Spunky throws a temper tantrum and proceeds to make things miserable for the animals on the farm despite Hunky's efforts to calm him. When the animals take matters into their own hands and begin to torment Spunky for his behavior, Hunky comes back to save her son.
A young horse says hi to little donkey Spunky. But the horse's mother pulls him away, saying she doesn't associate with that kind. Spunky makes a few more overtures, and eventually they set off on a chase, running across a bull from time to time. The horse stops to eat a lot of apples and drink far too much water; this leaves him too bloated to move much at all. The two continue to anger the bull, which gives chase; Spunky saves the colt, and they all live in harmony.
Hunky and Spunky are settling in for a nap, but a horse fly sees them and sees dinner. After battling the fly for a while, the youngster enlists mom's help. But the fly is merely stunned, and rallies a new attack, this time with friends. Hunky eventually kills the lot of them.