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Hyphenation may refer to:
- Use of the hyphen, a punctuation mark
- Syllabification, the splitting of words into smaller units (usually in printed texts, to achieve good line breaks)
- Hyphenation algorithm, an algorithm doing automatic syllabification
- In Hyphenation (analytical chemistry), the (online) coupling of analytical separation and detection technologies, e.g. Liquid chromatography–mass spectrometry (LC-MS).
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