Holworthy exhibited at the Royal Academy in 1803 and 1804. In the latter year he was one of the foundation members of the Society of Painters in Water-colours, now known as the Royal Watercolour Society,and he contributed constantly to their exhibitions till 1813. His subjects being drawn from Wales, the Lake district, and Yorkshire. He practised in London till 1822. In 1900, there were two drawings by him at the South Kensington Museum.
In 1821, Holworthy was living in Barton in the Beans, Leicestershire. In 1821 he married Anne Wright, a niece of Joseph Wright of Derby in Derby, and retired to the Brookfield estate, near Hathersage in Derbyshire, which he had purchased. He died in London in 1841, was buried at Kensal Green. He was a friend of J. M. W. Turner, R.A.
- "Tate Collection – Landscape Study by James Holworthy". The Tate. Retrieved 2008-08-21.
- Holworthy m. Wright at FamilySearch
- Marriage settlement contained in Papers of Cammell Deeds: I Brookfield Estate, including North Lees at the Sheffield Archives. Note that numerous papers relating to James Holworthy and the Wright family are contained in Cammell collection.
- Holworthy, James - index entry of death at FreeBMD
- Selby Whittingham "From Regence to Regency" Pantheon, 1984
- Redgrave, Samuel (1877). A descriptive catalogue of the historical collection of water-colour paintings in the South Kensington museum. London: Chapman and Hall. Retrieved 2008-08-21.
- Bryan’s Dict. (Graves);
- Grave’s Dict.;
- Catalogue of South Kensington Museum.
- James Holworthy in National Register of Archives and includes correspondence.