He studied law in Montpellier, during which time, he developed a passion for botany. He was a friend and collaborator to Scipio Maffei, with whom he took an extended scientific tour throughout Europe (1732–36). In 1755 he became a member of the Académie de Nîmes, serving as its secrétaire perpétuel from 1765 to 1784. In 1772 he became a member of the Académie royale des Inscriptions et Belles-Lettres.
His written works include a detailed description of the flora in the vicinity of Verona, titled Plantae Veronenses, seu Stirpium quae in agro Veronensi repriuntur (3 volumes 1745–54). Other noted works associated with Séguier are:
- Bibliotheca botanica, sive, Catalogus auctorum et librorum omnium qui de re botanica, de medicamentis ex vegetabilibus paratis, de re rustica, & de horticultura tractant, 1740.
- Dissertation sur l'ancienne inscription de la Maison-carrée de Nismes, 1759 – Dissertation on the ancient inscriptions of the Maison Carrée in Nîmes.
- Jean-François Séguier, Pierre Baux, lettres : 1733-1756.
- Jean GAUDANT Les poissons pétrifiés du Monte Bolca Annales.org
- SEGUIER Jean-François Sociétés savantes de France
- Jean-François Séguier, un naturaliste nimois Nemausensis
- Euphorbia seguieriana Neck. Acta plantarum
- Plantae veronenses OCLC WorldCat
- Most widely held works by Jean-François Séguier WorldCat Identities
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