June Almeida

June Dalziel Almeida
Born	June Dalziel Hart (1930-10-05)5 October 1930 Glasgow, Scotland
Died	1 December 2007(2007-12-01) (aged 77) Bexhill, England
Nationality	Scottish
Citizenship	British
Known for	Pioneer of immuno-electron microscopy
Spouse(s)	Enriques Rosalio (Henry) Almeida ​ ​ (m. 1954)​ Phillip Samuel Gardner ​ ​ (m. 1982⁠–⁠1994)​ (deceased)
Children	1
Scientific career
Fields	Virology, histopathology
Institutions	Glasgow Royal Infirmary, St Bartholomew's Hospital, Ontario Cancer Institute, Royal Postgraduate Medical School, Wellcome Research Laboratory

June Dalziel Almeida (5 October 1930 – 1 December 2007) was a Scottish virologist, a pioneer in virus imaging, identification and diagnosis. Her skills in electron microscopy earned her an international reputation.

In 1964, Almeida was recruited by St Thomas's Hospital Medical School in London. By 1967, she had earned her Doctor of Science (Sc.D.) on the basis of her research and the resulting publications, while working in Canada, at Toronto's Ontario Cancer Institute and then in London at St Thomas. She then continued her research at the Royal Postgraduate Medical School (RPGMS), which later became part of the Imperial College School of Medicine.

She succeeded in identifying viruses that were previously unknown, including—in 1966—a group of viruses that was later named coronavirus. Her immune electron microscopy (IEM) innovations and insights contributed to research related to the diagnosis of hepatitis B, HIV, and rubella, among other viral diseases. Her electron micrographs continue to be included in virology review textbooks, decades after she produced them.

Major contributions

In their 2013 book entitled To Catch a Virus, John Booss and Marilyn J. August describe how Almeida "played a crucial role in adapting the electron microscope to clinical diagnostic virology work."

Prior to the 1960s work of Almeida and Anthony Peter Waterson, very few improvements had been made on the initial 1941 "proof of principle aggregation of virus by virus-specific antibody observable by [electron microscope (EI)]." In 1963, Almeida pioneered a technique in immune electron microscopy (IEM), to better visualise viruses by using antibodies to aggregate them. In the 1960s, Almeida and Waterson were using negative staining for the EM of viruses—a technique that was both rapid and simple—and provided excellent detailed observations of viral morphology, which had revolutionized the electron microscopy of viruses overnight.

In 1966, using her new techniques, Almeida was able to identify a group of "previously uncharacterised human respiratory viruses", while collaborating with David Tyrrell, then director of the Common Cold Research Centre in Salisbury in Wiltshire. Tyrrell suggested calling the new group "coronaviruses". The coronavirus family of viruses now includes the SARS virus and the SARS-CoV2 virus that causes COVID-19.

In 1967, using the IEM aggregation method, she produced the first visualization of rubella virus.

Biography

June Dalziel Hart was born on 5 October 1930 at 10 Duntroon Street, Glasgow to Jane Dalziel (née Steven) and Harry Leonard Hart, a bus driver.

In 1947, when she was 16, she left school, and in spite of her academic strength, she did not have the financial means to attend university at that time. She began working as a histopathology technician, first, at the Glasgow Royal Infirmary, and then at St Bartholomew's Hospital, where she worked until 1954.

Ontario Cancer Institute

In 1954, she was hired for a newly-opened position as electron microscopy technician at the Ontario Cancer Institute, where she worked for ten years.

While working as an electronmicroscopist, she and her Cancer Institute colleagues produced a series of studies applying negative staining to clinical problems.

In 1963 she was the first of three authors who co-published an article in the journal Science, in which they identified virus-like particles in cancer patients' blood.

In 1963, she co-published her research, in which she "negatively stained aggregates of antigen...and antibody" with the electron microscope.

St Thomas's Hospital Medical School

Main article: St Thomas's Hospital Medical School

By 1964, Almeida had earned her Doctor of Science (Sc.D.), based on her publications of her research undertaken at the Ontario Cancer Institute and at St Thomas on electron micrographic research of antibodies.

In 1964, Tony Waterson, who had just been appointed as chair of microbiology at St Thomas, met Almeida while visiting Toronto, and recruited her to join his research team at one of the oldest and most prestigious medical schools in the United Kingdom—London's St Thomas's Hospital Medical School, now part of King's College London. At St Thomas, Almeida worked on hepatitis B virus and the cold viruses.

File:Virus surrounded by a distinct 200 A long fringe by Almeida and Tyrrell 1966.png

Electron micrograph of "corona" (crown) virus produced by Almeida in 1966

In 1966, Waterson and Almeida collaborated with the physician and director of research on the common cold, David Tyrrell, who was working on a new organ culture system. Tyrrell's team had been attempting to detect the presence of rhinoviruses in tissue cultures of cells that they had produced in the lab. They wanted to detect a specific respiratory virus they called B814. The Swedish professor Bertil Hoorn could make all Tyrrell's respiratory viruses in organ cultures of cells from the human airway in the laboratory, except for virus B814. This organ culture system meant they did not have to depend on human volunteers to do research on these viruses. They wanted a reliable method to detect virus B814.

In the book Cold Wars (2002), which Tyrrell wrote with Michael Fielder, he described how when he first met Almeida, she seemed to be extending the range of the electron microscope to new limits. According to Tyrrell, prior to Almeida's innovative work, it was generally accepted that viruses had to be concentrated and purified to detect them with the electron microscope. When Almeida told Tyrrell that she could "find virus particles" in the organ cultures that they had collected, with her "new, improved techniques", he was skeptical.

Tyrrell's team sent samples to Almeida in London. These included a sample prepared with the B814 virus, along with samples infected with influenza and herpes, which were well-known. When Almeida examined the samples through her microscope grids, "she recognized all the known viruses, and her pictures revealed their structure beautifully. But more importantly, she also saw virus particles in the B814 sample." She told Tyrrell that the B814 specimens had reminded her of particles she had previously studied in a "disease called infectious bronchitis of chickens" and in another disease—"mouse hepatitis liver inflammation." Her papers on these had been rejected because the referees considered her electron micrographs to be "bad pictures" of known influenza virus particles. She told him she now knew that these "three viruses were something quite new."

According to Tyrrell, once Almeida had identified the previously unrecognised group of viruses, they met in Waterson's office, to decide on its name. The viruses appeared to be surrounded by a "halo", which in Latin is "corona", and the name "coronavirus" was born.

In 1966, Almeida and Tyrrell wrote that, "The particles are pleomorphic, in the size range 800 to 1200 Å, and are surrounded by a distinct 200 Å long fringe. They are indistinguishable from the particles of avian infectious bronchitis, the only virus previously known to have this morphology."

Royal Postgraduate Medical School of London (RPGMS)

Three years later, in 1967, when Waterson took a position at the Royal Postgraduate Medical School (RPGMS), she also moved to begin work there.

In 1968, she co-published an article in Journal of General Virology, on "avian infectious bronchitis virus".

In 1971, using her immune electronmicroscopy technique, she made the landmark discovery that the hepatitis B virus had "two immunologically distinct components"—an "outer coat and a small inner component".

Wellcome Institute

She spent her final years of her professional career at the Wellcome Institute before she retired. While working for Wellcome she was named on several patents in the field of imaging viruses.

In retirement

After retiring from Wellcome Institute, she returned in an advisory role in the late 1980s when she helped produce micrographs of the HIV virus.

Her publications include the 1979 Manual for rapid laboratory viral diagnosis for the World Health Organisation.

Influences

At the Royal Postgraduate Medical School (RPGMS), in 1970, Almeida taught Albert Kapikian the technique of immune electron microscopy (IEM). Kapikian, who was visiting for six months from the United States National Institutes of Health, used her techniques in the identification of a cause of non-bacterial gastroenteritis—the Norwalk virus, now known as the Norovirus.

Almeida's work received new attention during the early months of the 2020 coronavirus pandemic. Her little-known story was first published by the Herald in Scotland on 7 March, the BBC on 15 April, the National Geographic on April 17. According to professor of bacteriology Hugh Pennington, Chinese scientists credited her work, including techniques she developed, with the early identification of COVID-19. Pennington himself called Almeida his "mentor".

Personal life

Almeida moved to Toronto, Ontario and worked at the Cancer Institute after her marriage on December 11, 1954 to Enrique Rosalio (Henry) Almeida (1913–1993), a Venezuelan artist with whom she had a daughter, Joyce.

Her daughter Joyce, a psychiatrist, has two daughters.

Her first marriage ended in divorce in 1982.

She retired in 1985 to Bexhill-on-Sea with her second husband, Phillip Samuel Gardner, a virologist.

Almeida died in Bexhill from a heart attack in 2007.

See also

• Timeline of women in science