M49, M.49 or M-49 may be:
- Messier 49, an elliptical galaxy in the constellation Virgo
- 49th known Mersenne prime
- M49 motorway, a motorway in Great Britain
- M49 telescope, a telescope used by the United States military
- M-49 (Michigan highway), a Michigan state highway
- UN M.49, a standard used by the United Nations for area codes
- M/49, or the Neuhausen pistol, the Danish name for the SIG Sauer P210
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