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|Look up Middlesex in Wiktionary, the free dictionary.|
Middlesex is a former county in southeast England.
- Middlesex, Belize, a village in Stann Creek District, Belize
- Middlesex, New Jersey
- Middlesex, New York
- Middlesex, North Carolina
- Middlesex, Vermont
- Middlesex, Virginia
- Middlesex Township, Butler County, Pennsylvania
- Middlesex Township, Cumberland County, Pennsylvania
- Middlesex School, Concord, Massachusetts
- Middlesex Canal, Massachusetts
- Middlesex County, Connecticut
- Middlesex County, Massachusetts
- Middlesex County, New Jersey
- Middlesex County, Virginia
- Middlesex (novel), by Jeffrey Eugenides
- Middlesex Plat Historic District, listed on the National Register of Historic Places in Polk County, Iowa
- Middlesex (1783 EIC ship), an East Indiaman of the British East India Company
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