The museum was established in 1980 by Thomas J. Witt. His well-known family founded Witt Weiden, one of Germany's oldest mail-order houses.
Since 2000 the museum has been part of the Zoologische Staatssammlung München (The Bavarian State Collection of Zoology) in Munich. The collection consists of about ten million specimens from all over the world. It is the largest collection of moths in the world. The Museum Witt has a collection of three to three and a half million butterflies, and the Bavarian State Collection of Zoology additional seven million butterflies. A crew of scientists work at the museum and it has an impressive library.