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N400 or N-400 may refer to:
- N400 (neuroscience), an event-related potential component elicited by meaningful stimuli (words, pictures, etc.)
- N-400 road (Spain), a highway connecting Toledo to Cuenca
- Honda N400, a car similar to the Honda N360
- Samsung N400, a mobile phone by Samsung
- Form N-400, United States Citizenship and Immigration Services application for naturalization
- A variant of the Aston Martin V8 Vantage car
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