Nucleobindin 1
Appearance
Nucleobindin-1 (NUCB1), also known as calnuc, is a protein that in humans is encoded by the NUCB1 gene.[5][6]
Structure
The human calnuc protein contains 461 amino acids and has a pI of 4.9.[7]
The protein contains the following regions and domains:
- Signal peptide[8]
- Leucine-zipper[8] The leucine-zipper of calnuc may be responsible for its dimerization.
- aa 347-389 – Nuclear localization signal
- 2 zinc binding sites[9] with high affinity (Kd = 20 nM)
- carboxypeptidase-like motifs : HFREXnH (aa 66) and HFTEXnH (aa 146)
- 2 EF-hands
- Potential anchor region[7]– 15-aa hydrophobic region at its COOH terminus that may be sufficient for membrane anchoring
References
- ^ a b c GRCh38: Ensembl release 89: ENSG00000104805 – Ensembl, May 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000030824 – Ensembl, May 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Miura K, Hirai M, Kanai Y, Kurosawa Y (Feb 1997). "Organization of the human gene for nucleobindin (NUC) and its chromosomal assignment to 19q13.2-q13.4". Genomics. 34 (2): 181–6. doi:10.1006/geno.1996.0263. PMID 8661046.
- ^ "Entrez Gene: NUCB1 nucleobindin 1".
- ^ a b Lin P, Le-Niculescu H, Hofmeister R, McCaffery JM, Jin M, Hennemann H, McQuistan T, De Vries L, Farquhar MG (June 1998). "The mammalian calcium-binding protein, nucleobindin (CALNUC), is a Golgi resident protein". J. Cell Biol. 141 (7): 1515–27. doi:10.1083/jcb.141.7.1515. PMC 2132997. PMID 9647645.
- ^ a b Miura K, Titani K, Kurosawa Y, Kanai Y (August 1992). "Molecular cloning of nucleobindin, a novel DNA-binding protein that contains both a signal peptide and a leucine zipper structure". Biochem. Biophys. Res. Commun. 187 (1): 375–80. doi:10.1016/S0006-291X(05)81503-7. PMID 1520323.
- ^ Kanuru M, Raman R, Aradhyam GK (January 2013). "Serine protease activity of calnuc: regulation by Zn2+ and G proteins". J. Biol. Chem. 288 (3): 1762–73. doi:10.1074/jbc.M112.382846. PMID 23195954.
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Further reading
- Miura K, Titani K, Kurosawa Y, Kanai Y (1992). "Molecular cloning of nucleobindin, a novel DNA-binding protein that contains both a signal peptide and a leucine zipper structure". Biochem. Biophys. Res. Commun. 187 (1): 375–80. doi:10.1016/S0006-291X(05)81503-7. PMID 1520323.
- Mochizuki N, Hibi M, Kanai Y, Insel PA (1995). "Interaction of the protein nucleobindin with G alpha i2, as revealed by the yeast two-hybrid system". FEBS Lett. 373 (2): 155–8. doi:10.1016/0014-5793(95)01031-9. PMID 7589456.
- Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
- Ballif BA; Mincek NV; Barratt JT; et al. (1996). "Interaction of cyclooxygenases with an apoptosis- and autoimmunity-associated protein". Proc. Natl. Acad. Sci. U.S.A. 93 (11): 5544–9. doi:10.1073/pnas.93.11.5544. PMC 39283. PMID 8643612.
{{cite journal}}
: Unknown parameter|name-list-format=
ignored (|name-list-style=
suggested) (help) - Suzuki Y; Yoshitomo-Nakagawa K; Maruyama K; et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
{{cite journal}}
: Unknown parameter|name-list-format=
ignored (|name-list-style=
suggested) (help) - Kubota T; Miyauchi M; Miura K; et al. (1998). "Upregulation of nucleobindin expression in human-activated lymphocytes and non-Hodgkin's lymphoma". Pathol. Int. 48 (1): 22–8. doi:10.1111/j.1440-1827.1998.tb03823.x. PMID 9589460.
{{cite journal}}
: Unknown parameter|name-list-format=
ignored (|name-list-style=
suggested) (help) - Lin P; Le-Niculescu H; Hofmeister R; et al. (1998). "The mammalian calcium-binding protein, nucleobindin (CALNUC), is a Golgi resident protein". J. Cell Biol. 141 (7): 1515–27. doi:10.1083/jcb.141.7.1515. PMC 2132997. PMID 9647645.
{{cite journal}}
: Unknown parameter|name-list-format=
ignored (|name-list-style=
suggested) (help) - Taniguchi N; Taniura H; Niinobe M; et al. (2000). "The postmitotic growth suppressor necdin interacts with a calcium-binding protein (NEFA) in neuronal cytoplasm". J. Biol. Chem. 275 (41): 31674–81. doi:10.1074/jbc.M005103200. PMID 10915798.
{{cite journal}}
: Unknown parameter|name-list-format=
ignored (|name-list-style=
suggested) (help)CS1 maint: unflagged free DOI (link) - Weiss TS; Chamberlain CE; Takeda T; et al. (2002). "Galpha i3 binding to calnuc on Golgi membranes in living cells monitored by fluorescence resonance energy transfer of green fluorescent protein fusion proteins". Proc. Natl. Acad. Sci. U.S.A. 98 (26): 14961–6. doi:10.1073/pnas.261572098. PMC 64966. PMID 11752444.
{{cite journal}}
: Unknown parameter|name-list-format=
ignored (|name-list-style=
suggested) (help) - Strausberg RL; Feingold EA; Grouse LH; et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
{{cite journal}}
: Unknown parameter|name-list-format=
ignored (|name-list-style=
suggested) (help) - Ota T; Suzuki Y; Nishikawa T; et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
{{cite journal}}
: Unknown parameter|name-list-format=
ignored (|name-list-style=
suggested) (help) - de Alba E, Tjandra N (2004). "Structural studies on the Ca2+-binding domain of human nucleobindin (calnuc)". Biochemistry. 43 (31): 10039–49. doi:10.1021/bi049310a. PMID 15287731.
- Breuza L; Halbeisen R; Jenö P; et al. (2004). "Proteomics of endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46". J. Biol. Chem. 279 (45): 47242–53. doi:10.1074/jbc.M406644200. PMID 15308636.
{{cite journal}}
: Unknown parameter|name-list-format=
ignored (|name-list-style=
suggested) (help)CS1 maint: unflagged free DOI (link) - Gerhard DS; Wagner L; Feingold EA; et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
{{cite journal}}
: Unknown parameter|name-list-format=
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suggested) (help) - Rual JF; Venkatesan K; Hao T; et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
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: Unknown parameter|name-list-format=
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suggested) (help) - Chen Y; Lin P; Qiu S; et al. (2007). "Autoantibodies to Ca2+ binding protein Calnuc is a potential marker in colon cancer detection". Int. J. Oncol. 30 (5): 1137–44. doi:10.3892/ijo.30.5.1137. PMID 17390015.
{{cite journal}}
: Unknown parameter|name-list-format=
ignored (|name-list-style=
suggested) (help) - Tsukumo Y; Tomida A; Kitahara O; et al. (2007). "Nucleobindin 1 controls the unfolded protein response by inhibiting ATF6 activation". J. Biol. Chem. 282 (40): 29264–72. doi:10.1074/jbc.M705038200. PMID 17686766.
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