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ROSA26 is a locus used for constitutive, ubiquitous gene expression in mice.[1] It was first isolated in 1991[2] in a gene-trap mutagenesis screen of embryonic stem cells (ESCs). Over 130 knock-in lines have been created based on the ROSA26 locus.[3] The human homolog of the ROSA26 locus has been identified.[4]


  1. ^ "rosa26". Retrieved 8 January 2013.
  2. ^ Friedrich, G; Soriano, P (1991). "Promoter traps in embryonic stem cells: A genetic screen to identify and mutate developmental genes in mice". Genes & Development. 5 (9): 1513–23. doi:10.1101/gad.5.9.1513. PMID 1653172.
  3. ^ Casola, S (2010). "Mouse models for miRNA expression: The ROSA26 locus". Methods in Molecular Biology. Methods in Molecular Biology. 667: 145–63. doi:10.1007/978-1-60761-811-9_10. ISBN 978-1-60761-810-2. PMID 20827532.
  4. ^ Irion, Stefan; Luche, Hervé; Gadue, Paul; Fehling, Hans Joerg; Kennedy, Marion; Keller, Gordon (2007). "Identification and targeting of the ROSA26 locus in human embryonic stem cells". Nature Biotechnology. 25 (12): 1477–82. doi:10.1038/nbt1362. PMID 18037879.