The spermatid is the haploid male gametid that results from division of secondary spermatocytes. As a result of meiosis, each spermatid contains only half of the genetic material present in the original primary spermatocyte.
Spermatids are connected by cytoplasmic material and have superfluous cytoplasmic material around their nuclei.
The spermatids begin to grow a living thread, develop a thickened mid-piece where the mitochondria become localised, and form an acrosome. Spermatid DNA also undergoes packaging, becoming highly condensed. The DNA is packaged firstly with specific nuclear basic proteins, which are subsequently replaced with protamines during spermatid elongation. The resultant tightly packed chromatin is transcriptionally inactive.
In 2016 scientists at Nanjing Medical University claimed they had produced cells resembling mouse spermatids artificially from stem cells. They injected these spermatids into mouse eggs and produced pups.
As postmeiotic germ cells develop to mature sperm they progressively lose the ability to repair DNA damage that may then accumulate and be transmitted to the zygote and ultimately the embryo. In particular, the repair of DNA double-strand breaks by the non-homologous end joining pathway, although present in round spermatids, appears to be lost as they develop into elongated spermatids.
- Cyranoski, David (25 February 2016). "Researchers claim to have made artificial mouse sperm in a dish". Nature. doi:10.1038/nature.2016.19453. Retrieved 4 March 2016.
- Marchetti F, Wyrobek AJ (2008). "DNA repair decline during mouse spermiogenesis results in the accumulation of heritable DNA damage". DNA Repair (Amst.). 7 (4): 572–81. doi:10.1016/j.dnarep.2007.12.011. PMID 18282746.
- Ahmed EA, Scherthan H, de Rooij DG (2015). "DNA Double Strand Break Response and Limited Repair Capacity in Mouse Elongated Spermatids". Int J Mol Sci. 16 (12): 29923–35. doi:10.3390/ijms161226214. PMC 4691157. PMID 26694360.