THP-1 cell line

From Wikipedia, the free encyclopedia
  (Redirected from THP1 cell line)
Jump to: navigation, search

THP1 is a human monocytic cell line derived from an acute monocytic leukemia patient. It is used to test leukemia cell lines in immunocytochemical analysis of protein-protein interaction, and immunohistochemistry.

Cell Characteristics Growth Information[edit]

Note that although THP-1 cells are of the same lineage, mutations can cause differences as the progeny proliferates.

Characteristics[edit]

These cells show a large, round, single-cell morphology.

They are derived from the peripheral blood of a 1 year old human male with acute monocytic leukemia. Some of their characteristics are:

DQB1*05, DQB1*06.

Growth Information[edit]

This cell line can provide continuous culture, grown in suspension; RPMI 1640 + 10% FBS + 2mM L-Glutamine. The average doubling time is 35 to 50 hours. You may also add 1 mM sodium pyruvate, penicillin (100 units/ml) and streptomycin (100 μg/ml) to inhibit bacterial contamination. Maintain cultures between 2-9x105 cells/ml. 37C, 5% CO2. Cells are non-adhesive.

Hazard: Cell line of human origin. There is no evidence for the presence of infectious viruses or toxic products. However, it is recommended that cultures are handled under Biosafety Level 2 containment.

References[edit]

  • Tsuchiya S, Yamabe M, Yamaguchi Y, Kobayashi Y, Konno T, Tada K (August 1980). "Establishment and characterization of a human acute monocytic leukemia cell line (THP-1)". Int. J. Cancer 26 (2): 171–6. doi:10.1002/ijc.2910260208. PMID 6970727.