Talk:Thermal shift assay

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Feb 2021

Will someone please explain what GTP stands for in DSF-GTP? — Preceding unsigned comment added by Eric Martz (talk • contribs) 22:53, 8 February 2021 (UTC)[reply]

May 2015

I like this article but the scope is too limited. In Groftehauge et al 2015 ^[1] thermal shift assay is defined as any high-throughput assay that evaluates the melting temperature of a protein under various conditions (ligands, salts, pH etc). The researcher investigates the shift in thermal denaturation temperature.

Here we only have a fluorescent assay, usually performed with SYPRO Orange. Originally called thermofluor assay but at some point Frank Niesen dubbed it Differential Scanning Fluorimetry (but any time you say that to anyone they confuse it with Differential Scanning Calorimetry). Thermofluor is a fine name, it is no longer trademarked and it is unambiguous. Giving it a third name is not helpful. Especially not when that third name better describes a collection of assays with similar outcomes.

No mention of things like FastPP (fast parallel proteolysis) or differential light scattering. Or a bunch of other methods.

Can I just start writing it? It seems like a major rewrite of someone else's text so I am hesitant. Just to be clear I don't want to rename the page. I want the thermofluor assay and others to be subsections on page about thermal shift assays.

Structurebiologist (talk) 14:06, 21 May 2015 (UTC)[reply]

References

^ http://journals.iucr.org/d/issues/2015/01/00/ba5217/index.html

Editing

There was recently some very heavy editing that removed quite a bit of useful and correct information. I am going to revert it, but am starting this conversation where we can what to include/exclude and if anything should be moved into separate articles. Many of the additions/changes are useful but should be classified under applications rather than deleting other relevant methods. Dbsseven (talk) 17:05, 16 November 2016 (UTC)[reply]

[1] ttp://journals.iucr.org/d/issues/2015/01/00/ba5217/index.html

[1]