Tobiah was an Ammonite official (possibly a governor of Ammon, possibly also of Jewish descent) who incited the Ammonites to hinder Nehemiah's efforts to rebuild Jerusalem. He, along with Sanballat the Horonite and Geshem the Arabian, resorted to a stratagem and, pretending to wish a conference with Nehemiah, invited him to meet them at Ono, Benjamin. Four times they made the request, and every time Nehemiah refused to come. Their object was to frighten him from completing the restoration of Jerusalem's walls and to do him some kind of harm.
Tobiah also had married a daughter of Shecaniah, a Judahite leader, and had given his son, Jehohanan, in marriage to the daughter of Meshullam, another Judahite leader, for ostensibly political purposes. Because of this, he somehow gained enough of a Judahite coalition to use the Judahites themselves to send letters to Nehemiah, telling him of Tobiah's "good deeds" in an apparent attempt to weaken Nehemiah's resolve to keep Tobiah out of the rebuilding effort. Tobiah meanwhile sent intimidating letters directly to Nehemiah.
Additionally, Tobiah had established a close relationship with High Priest Eliashib, whose grandson had married the daughter of Sanballat such that Eliashib leased the storerooms of the temple, intended to be filled with the Israelite's grain offerings, incense, temple articles, and the tithes of grain, new wine and oil meant for the work of the temple and the temple workers themselves, so that Tobiah could conduct business in the newly constructed temple. Upon hearing this, Nehemiah, who was then in Babylon serving Artaxerxes I of Persia, requested permission to return to Judah. After returning, he promptly threw all of Tobiah's belongings out of the temple room, purified the room, and put back all that had originally been there.
It is possible, though uncertain, that Tobiah the Ammonite may be related to other Tobiahs mentioned in extra-biblical sources. The Lachish Ostraca  mention a Tobiah who is a "servant of the king". Josephus later mentions a rich and influential Tobiad family from around the geographic region of Ammon, which may be descended from or otherwise related to this same Tobiah.