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Lysozyme treatment, l lysis, gram negative and positive bacteria, ---> spheroplasts.

Look for conditions, for optimal lysozyme lysis, in the laboratory.

Lysozyme treatment is optimal at particular temperatures, pH ranges, and salt concentrations. Lysozyme activity increases with increasing temperatures, up to 60 degrees Celsius, with a pH range of 6.0-7.0. The salts present also affect lysozyme treatment, where some assert inhibitory effects, and others promote lysis via lysozyme treatment. Sodium chloride induces lysis, but at high concentrations, it is an active inhibitor of lysis. Similar observations have been seen with the use of potassium salts. Slight variations are present due to differences in bacterial strains.

Lysozyme is a commonly used enzyme for lysing gram negative bacteria.

Final Edit: Group Members: Sonia Amanat, Adrian Argudo, Salvatore DiBono, Abdullah Mayan

Final Edited Paragraph

On March 21, 1937, a peaceful march was organized by the Nationalist Party, under Pedro Alibizu Campos, to commemorate the ending of slavery in Puerto Rico in 1873 by the governing Spanish National Assembly.[69] The police, under the orders of General Blanton Winship, the US-appointed colonial Governor of Puerto Rico, opened fire at the peaceful Puerto Rican Nationalist Party parade, which is now known as the "Ponce massacre": 20 unarmed people (including two policemen) were killed,[70][71] with wounded persons, ranging between 100-200.[70][71]

Bold: Sonia Amanat

Orignal text mentioned there were 19 persons killed, several resources have stated 20, two of which have been referenced, within the wiki edit

Sources

70. Navarro, Mireya (2003-11- 28). “Decades of Surveillance of Puerto Rican Groups.” World Scholar: Latin America & the Caribbean. New York Times

71. Hays, Arthur (1937). “Report of the Commission of Inquiry on Civil Rights in Puerto Rico”. 1: 70.

72. “Colonialism: Native American and Puerto Rican.”. Akwesasne Notes (1975-1989). Rooseveltown. 7

Original Text: On March 21, 1937, a peaceful march was organized by the Nationalist Party (1)  to commemorate the ending of slavery in Puerto Rico in 1873 by the governing Spanish National Assembly (4) The police, under the orders of General Blanton Winship, the US-appointed colonial Governor of Puerto Rico, opened fire at the peaceful Puerto Rican Nationalist Party parade, bringing about what came to be known as the "Ponce massacre": 19 people (2) (including two policemen) were killed and over 100 were wounded (3)

Edited Text: Original Text: On March 21, 1937, a peaceful march was organized by the Nationalist Party leader, Pedro Alibizu Campos, to commemorate the ending of slavery in Puerto Rico in 1873 by the governing Spanish National Assembly. The police, under the orders of General Blanton Winship, the US-appointed colonial Governor of Puerto Rico, opened fire at the peaceful Puerto Rican Nationalist Party parade, bringing about what came to be known as the "Ponce massacre": 20 unarmed people (2) (including two policemen) were killed and with a conflicting number of wounded, ranging between 100-200 (3)

AFPRL WIKI ASSIGNMENT

  • Confirmation of leaders.
  • Adding new leaders, as per needed.
  • Confirmation of stats; making stats more precise
  • Confirmation of dates

2 articles on the Nationalist leader Pedro Alibizu Campos.

1.http://ic.galegroup.com/ic/gic/BiographiesDetailsPage/BiographiesDetailsWindow?disableHighlighting=false&displayGroupName=Biographies&currPage=&scanId=&query=&source=&prodId=GIC&search_within_results=&p=GIC%3AOVIC&mode=view&catId=&u=cuny_hunter&limiter=&display-query=&displayGroups=&contentModules=&action=e&sortBy=&documentId=GALE%7CK3444700039&windowstate=normal&activityType=&failOverType=&commentary=

Discusses about Pedro Alibizu Campos and his life, and relationship with the United States. Article briefly mentions his change in political views and his influence toward the nationalist Party.

2.http://worldscholar.galegroup.com/region/latin-america/NewsDetailsPage/NewsDetailsWindow?disableHighlighting=false&displayGroupName=News&currPage=&scanId=&query=&source=&prodId=LAC&search_within_results=&p=LACD%3ALACP&mode=view&catId=&u=cuny_hunter&limiter=&display-query=&displayGroups=&contentModules=&action=e&sortBy=&documentId=GALE%7CA112951298&windowstate=normal&activityType=&failOverType=&commentary=

Primarily mentions FBI and the American government involvement Pedro Alibizu Campos. Article also mentions Pedro Alibizu Campos was head of the Nationalist party from 1930-1965.

2 articles on statistics *2 secondary sources

http://search.proquest.com/ethnicnewswatch/docview/220292038/D953BDBEA50C4A75PQ/6?accountid=27495 (unarmed)

http://search.proquest.com/ethnicnewswatch/docview/222594498/D953BDBEA50C4A75PQ/7?accountid=27495 (describes that they were 20 unarmed civilians)

1 article on Ponce Massacre *Primary source http://search.proquest.com/ethnicnewswatch/docview/192408073/4774F514B4E04FBBPQ/2?accountid=27495  (describes what a civilian saw the day of the massacre, also includes some historical context)

*Primary source- The Report on the Commission of Civil rights in Puerto Rico--  http://www.llmcdigital.org/docdisplay.aspx?textid=29121410&type=PDF   It discusses the events of the massacre and the events after the fact all they way to may 1937 (two months after the massacre)