Wyo or WYO may refer to:
- Wyoming, a state in USA
- Wyo, California, a community in Glenn County, California
- Wessex Youth Orchestra, an orchestra in Poole, England
- Williamsburg Youth Orchestra, an orchestra in Williamsburg, Virginia
- World Youth Organization, a non-profit organization in Chennai, India
- WYO, a candidate phylum of bacteria
disambiguation page lists articles associated with the title Wyo.
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