Zaire ebolavirus: Difference between revisions

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== Use of term ==
== Use of term ==
Ebola virus (abbreviated EBOV) was first described in 1976.<ref name=Pattyn1977>{{
Ebola virus (abbreviated EBOV) was first described in 1976.<ref name=Pattyn1977>{{cite journal
cite journal
|last1=Pattyn
|last1=Pattyn
|first1=S.
|first1=S.
Line 33: Line 32:
|pages = 573–4
|pages = 573–4
|pmid = 65663
|pmid = 65663
}}</ref><ref name=Bowen1977>{{
}}</ref><ref name=Bowen1977>{{cite journal
cite journal
|last1=Bowen
|last1=Bowen
|first1=E. T. W.
|first1=E. T. W.
Line 54: Line 52:
|pages = 571–3
|pages = 571–3
|pmid = 65662
|pmid = 65662
}}</ref><ref name=Johnson1977>{{
}}</ref><ref name=Johnson1977>{{cite journal
cite journal
|last1=Johnson
|last1=Johnson
|first1=K. M.
|first1=K. M.
Line 71: Line 68:
|pages = 569–71
|pages = 569–71
|pmid = 65661
|pmid = 65661
}}</ref> Today, the virus is the single member of the [[International Committee on Taxonomy of Viruses|species]] ''[[Zaire ebolavirus]]'', which is included into the [[International Committee on Taxonomy of Viruses|genus]] ''[[Ebolavirus]]'', [[International Committee on Taxonomy of Viruses|family]] ''[[Filoviridae]]'', [[International Committee on Taxonomy of Viruses|order]] ''[[Mononegavirales]]''. The name Ebola virus is derived from the [[Ebola River]] (a river that was at first thought to be in close proximity to the area in [[Zaire]] where the first recorded Ebola virus disease outbreak occurred) and the [[Taxonomy|taxonomic]] [[suffix]] ''virus''.<ref name=KuhnArch>{{
}}</ref> Today, the virus is the single member of the [[International Committee on Taxonomy of Viruses|species]] ''[[Zaire ebolavirus]]'', which is included into the [[International Committee on Taxonomy of Viruses|genus]] ''[[Ebolavirus]]'', [[International Committee on Taxonomy of Viruses|family]] ''[[Filoviridae]]'', [[International Committee on Taxonomy of Viruses|order]] ''[[Mononegavirales]]''. The name Ebola virus is derived from the [[Ebola River]] (a river that was at first thought to be in close proximity to the area in [[Zaire]] where the first recorded Ebola virus disease outbreak occurred) and the [[Taxonomy|taxonomic]] [[suffix]] ''virus''.<ref name=KuhnArch>{{cite journal
cite journal
|last1=Kuhn
|last1=Kuhn
|first1=Jens H.
|first1=Jens H.
Line 98: Line 94:
|pages = 2083–103
|pages = 2083–103
|doi = 10.1007/s00705-010-0814-x
|doi = 10.1007/s00705-010-0814-x
|pmid = 21046175
|pmid = 21046175
|pmc=3074192
|pmc=3074192
}}</ref> Ebola virus is pronounced ɛ’bɒlə vɑɪrəs ([[IPA]]) or eh-bo-luh v-eye-ruhs in English phonetic notation.<ref name=KuhnArch/> According to the rules for taxon naming established by the [[International Committee on Taxonomy of Viruses]] (ICTV), the name Ebola virus is always to be [[Capitalization|capitalized]], but is never [[Italic type|italicized]], and may be [[Abbreviation|abbreviated]] (with EBOV being the official abbreviation).
}}</ref> Ebola virus is pronounced ɛ’bɒlə vɑɪrəs ([[IPA]]) or eh-bo-luh v-eye-ruhs in English phonetic notation.<ref name=KuhnArch/> According to the rules for taxon naming established by the [[International Committee on Taxonomy of Viruses]] (ICTV), the name Ebola virus is always to be [[Capitalization|capitalized]], but is never [[Italic type|italicized]], and may be [[Abbreviation|abbreviated]] (with EBOV being the official abbreviation).


== Previous designations ==
== Previous designations ==
Ebola virus was first introduced as a possible new "strain" of [[Marburg virus]] in 1977 by two different research teams.<ref name=Pattyn1977/><ref name=Bowen1977/> At the same time, a third team introduced the name Ebola virus.<ref name=Johnson1977/> In 2000, the virus name was changed to Zaire Ebola virus,<ref>{{
Ebola virus was first introduced as a possible new "strain" of [[Marburg virus]] in 1977 by two different research teams.<ref name=Pattyn1977/><ref name=Bowen1977/> At the same time, a third team introduced the name Ebola virus.<ref name=Johnson1977/> In 2000, the virus name was changed to Zaire Ebola virus,<ref>{{Citation
Citation
|last1=Netesov
|last1=Netesov
|first1=S. V.
|first1=S. V.
Line 143: Line 138:
|publisher=Academic Press
|publisher=Academic Press
|location=San Diego, USA
|location=San Diego, USA
|isbn=0123702003
|isbn=0-12-370200-3
}}</ref><ref>{{
}}</ref><ref>{{cite journal
cite journal
|last1=Pringle
|last1=Pringle
|first1=C. R.
|first1=C. R.
Line 155: Line 149:
|pages = 1449–59
|pages = 1449–59
|pmid = 9742051
|pmid = 9742051
}}</ref> and in 2005 to Zaire ebolavirus.<ref name=Feldmann2005>{{
}}</ref> and in 2005 to Zaire ebolavirus.<ref name=Feldmann2005>{{Citation
Citation
|last1=Feldmann
|last1=Feldmann
|first1=H.
|first1=H.
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|publisher=Elsevier/Academic Press
|publisher=Elsevier/Academic Press
|location=San Diego, USA
|location=San Diego, USA
|isbn=0123702003
|isbn=0-12-370200-3
}}</ref><ref>{{
}}</ref><ref>{{cite journal
cite journal
|last1=Mayo
|last1=Mayo
|first1=M. A.
|first1=M. A.

Revision as of 14:57, 22 March 2012

Ebola virus (EBOV)
Ebola virus electron micrograph
Virus classification
Group:
Group V ((−)ssRNA)
Order:
Mononegavirales
Family:
Filoviridae
Genus:
Ebolavirus
Species:
Zaire ebolavirus

Ebola virus (EBOV) causes severe disease in humans and in nonhuman primates in the form of viral hemorrhagic fever. EBOV is a select agent, World Health Organization Risk Group 4 Pathogen (requiring Biosafety Level 4-equivalent containment), National Institutes of Health/National Institute of Allergy and Infectious Diseases Category A Priority Pathogen, Centers for Disease Control and Prevention Category A Bioterrorism Agent, and listed as a Biological Agent for Export Control by the Australia Group.

Use of term

Ebola virus (abbreviated EBOV) was first described in 1976.[1][2][3] Today, the virus is the single member of the species Zaire ebolavirus, which is included into the genus Ebolavirus, family Filoviridae, order Mononegavirales. The name Ebola virus is derived from the Ebola River (a river that was at first thought to be in close proximity to the area in Zaire where the first recorded Ebola virus disease outbreak occurred) and the taxonomic suffix virus.[4] Ebola virus is pronounced ɛ’bɒlə vɑɪrəs (IPA) or eh-bo-luh v-eye-ruhs in English phonetic notation.[4] According to the rules for taxon naming established by the International Committee on Taxonomy of Viruses (ICTV), the name Ebola virus is always to be capitalized, but is never italicized, and may be abbreviated (with EBOV being the official abbreviation).

Previous designations

Ebola virus was first introduced as a possible new "strain" of Marburg virus in 1977 by two different research teams.[1][2] At the same time, a third team introduced the name Ebola virus.[3] In 2000, the virus name was changed to Zaire Ebola virus,[5][6] and in 2005 to Zaire ebolavirus.[7][8] However, most scientific articles continued to refer to Ebola virus or used the terms Ebola virus and Zaire ebolavirus in parallel. Consequently, in 2010, the name Ebola virus was reinstated.[4] Previous abbreviations for the virus were EBOV-Z (for Ebola virus Zaire) and most recently ZEBOV (for Zaire Ebola virus or Zaire ebolavirus). In 2010, EBOV was reinstated as the abbreviation for the virus.[4]

Virus inclusion criteria

A virus of the species Zaire ebolavirus is an Ebola virus if it has the properties of Zaire ebolaviruses and if its genome diverges from that of the prototype Zaire ebolavirus, Ebola virus variant Mayinga (EBOV/May), by ≤10% at the nucleotide level.[4]

Disease

Main article: Ebola virus disease

EBOV is one of four ebolaviruses that causes Ebola virus disease (EVD) in humans (in the literature also often referred to as Ebola hemorrhagic fever, EHF). In the past, EBOV has caused the following EVD outbreaks:

Ebola virus disease (EVD) outbreaks due to Ebola virus (EBOV) infection
Year Geographic location Human cases/deaths (case-fatality rate)
1976 Yambuku, Zaire 318/280 (88%)
1977 Bonduni, Zaire 1/1 (100%)
1988 Porton Down, United Kingdom 1/0 (0%) [laboratory accident]
1994-1995 Woleu-Ntem and Ogooué-Ivindo Provinces, Gabon 52/32 (62%)
1995 Kikwit, Zaire 317/245 (77%)
1996 Mayibout 2, Gabon 31/21 (68%)
1996 Sergiyev Posad, Russia 1/1 (100%) [laboratory accident]
1996-1997 Ogooué-Ivindo Province, Gabon; Cuvette-Ouest Department, Republic of the Congo 62/46 (74%)
2001-2002 Ogooué-Ivindo Province, Gabon; Cuvette-Ouest Department, Republic of the Congo 124/97 (78%)
2002 Ogooué-Ivindo Province, Gabon; Cuvette-Ouest Department, Republic of the Congo 11/10 (91%)
2002-2003 Cuvette-Ouest Department, Republic of the Congo; Ogooué-Ivindo Province, Gabon 143/128 (90%)
2003-2004 Cuvette-Ouest Department, Republic of the Congo 35/29 (83%)
2004 Koltsovo, Russia 1/1 (100%) [laboratory accident]
2005 Cuvette-Ouest Department, Republic of the Congo 11/9 (82%)
2007 Kasai Occidental Province, Democratic Republic of the Congo 264/186 (71%)
2008-2009 Kasai Occidental Province, Democratic Republic of the Congo 32/15 (47%)

Virology

Structure

Electron micrographs of EBOV show them to have the characteristic threadlike structure of a filovirus.[9] EBOV VP30 is around 288 amino acids long.[10] The virions are tubular in general form but variable in overall shape and may appear as the classic shepherd's crook or eyebolt, as a U or a 6, or coiled, circular, or branched; laboratory techniques, such as centrifugation, may be the origin of some of these formations.[11] Virions are generally 80 nm in diameter with a lipid bilayer anchoring the glycoprotein which projects 7 to 10 nm long spikes from its surface.[12] They are of variable length, typically around 800 nm, but may be up to 1000 nm long. In the center of the virion is a structure called nucleocapsid, which is formed by the helically wound viral genomic RNA complexed with the proteins NP, VP35, VP30, and L.[13] It has a diameter of 80 nm and contains a central channel of 20–30 nm in diameter. Virally encoded glycoprotein (GP) spikes 10 nm long and 10 nm apart are present on the outer viral envelope of the virion, which is derived from the host cell membrane. Between envelope and nucleocapsid, in the so-called matrix space, the viral proteins VP40 and VP24 are located.[14]

Genome

Each virion contains one molecule of linear, single-stranded, negative-sense RNA, 18,959 to 18,961 nucleotides in length. The 3′ terminus is not polyadenylated and the 5′ end is not capped. It was found that 472 nucleotides from the 3' end and 731 nucleotides from the 5' end are sufficient for replication.[15] It codes for seven structural proteins and one non-structural protein. The gene order is 3′ – leader – NP – VP35 – VP40 – GP/sGP – VP30 – VP24 – L – trailer – 5′; with the leader and trailer being non-transcribed regions, which carry important signals to control transcription, replication, and packaging of the viral genomes into new virions. The genomic material by itself is not infectious, because viral proteins, among them the RNA-dependent RNA polymerase, are necessary to transcribe the viral genome into mRNAs because it is a negative sense RNA virus, as well as for replication of the viral genome. Sections of the NP and the L genes from filoviruses have been identified as endogenous in the genomes of several groups of small mammals.[16]

Replication

Being acellular, viruses do not grow through cell division; instead, they use the machinery and metabolism of a host cell to produce multiple copies of themselves, and they assemble in the cell.[13]

  • The virus attaches to host receptors through the glycoprotein (GP) surface peplomer and is endocytosed into macropinosomes in the host cell [17]
  • Viral membrane fuses with vesicle membrane, nucleocapsid is released into the cytoplasm
  • Encapsidated, negative-sense genomic ssRNA is used as a template for the synthesis (3' – 5') of polyadenylated, monocistronic mRNAs
  • Using the host cell's machinery translation of the mRNA into viral proteins occurs
  • Viral proteins are processed, glycoprotein precursor (GP0) is cleaved to GP1 and GP2, which are heavily glycosylated. These two molecules assemble, first into heterodimers, and then into trimers to give the surface peplomers. Secreted glycoprotein (sGP) precursor is cleaved to sGP and delta peptide, both of which are released from the cell.
  • As viral protein levels rise, a switch occurs from translation to replication. Using the negative-sense genomic RNA as a template, a complementary +ssRNA is synthesized; this is then used as a template for the synthesis of new genomic (-)ssRNA, which is rapidly encapsidated.
  • The newly formed nucleocapsids and envelope proteins associate at the host cell's plasma membrane; budding occurs, destroying the cell.

References

  1. ^ a b Pattyn, S.; Jacob, W.; van der Groen, G.; Piot, P.; Courteille, G. (1977). "Isolation of Marburg-like virus from a case of haemorrhagic fever in Zaire". Lancet. 309 (8011): 573–4. PMID 65663.
  2. ^ a b Bowen, E. T. W.; Lloyd, G.; Harris, W. J.; Platt, G. S.; Baskerville, A.; Vella, E. E. (1977). "Viral haemorrhagic fever in southern Sudan and northern Zaire. Preliminary studies on the aetiological agent". Lancet. 309 (8011): 571–3. PMID 65662.
  3. ^ a b Johnson, K. M.; Webb, P. A.; Lange, J. V.; Murphy, F. A. (1977). "Isolation and partial characterisation of a new virus causing haemorrhagic fever in Zaire". Lancet. 309 (8011): 569–71. PMID 65661.
  4. ^ a b c d e Kuhn, Jens H.; Becker, Stephan; Ebihara, Hideki; Geisbert, Thomas W.; Johnson, Karl M.; Kawaoka, Yoshihiro; Lipkin, W. Ian; Negredo, Ana I; Netesov, Sergey V. (2010). "Proposal for a revised taxonomy of the family Filoviridae: Classification, names of taxa and viruses, and virus abbreviations". Archives of Virology. 155 (12): 2083–103. doi:10.1007/s00705-010-0814-x. PMC 3074192. PMID 21046175.
  5. ^ Netesov, S. V.; Feldmann, H.; Jahrling, P. B.; Klenk, H. D.; Sanchez, A. (2000), "Family Filoviridae", in van Regenmortel, M. H. V.; Fauquet, C. M.; Bishop, D. H. L.; Carstens, E. B.; Estes, M. K.; Lemon, S. M.; Maniloff, J.; Mayo, M. A.; McGeoch, D. J.; Pringle, C. R.; Wickner, R. B. (eds.), Virus Taxonomy—Seventh Report of the International Committee on Taxonomy of Viruses, San Diego, USA: Academic Press, pp. 539–48, ISBN 0-12-370200-3
  6. ^ Pringle, C. R. (1998). "Virus taxonomy-San Diego 1998". Archives of Virology. 143 (7): 1449–59. PMID 9742051.
  7. ^ Feldmann, H.; Geisbert, T. W.; Jahrling, P. B.; Klenk, H.-D.; Netesov, S. V.; Peters, C. J.; Sanchez, A.; Swanepoel, R.; Volchkov, V. E. (2005), "Family Filoviridae", in Fauquet, C. M.; Mayo, M. A.; Maniloff, J.; Desselberger, U.; Ball, L. A. (eds.), Virus Taxonomy—Eighth Report of the International Committee on Taxonomy of Viruses, San Diego, USA: Elsevier/Academic Press, pp. 645–653, ISBN 0-12-370200-3
  8. ^ Mayo, M. A. (2002). "ICTV at the Paris ICV: results of the plenary session and the binomial ballot". Archives of Virology. 147 (11): 2254–60.
  9. ^ Klenk & Feldmann 2004, p. 2
  10. ^ Klenk & Feldmann 2004, p. 13
  11. ^ Klenk & Feldmann 2004, pp. 33–35
  12. ^ Klenk & Feldmann 2004, p. 28
  13. ^ a b Biomarker Database. Ebola virus. Korea National Institute of Health. Retrieved 2009-05-31.
  14. ^ Attention: This template ({{cite pmid}}) is deprecated. To cite the publication identified by PMID 8219816, please use {{cite journal}} with |pmid=8219816 instead.
  15. ^ Klenk & Feldmann 2004, p. 9
  16. ^ Attention: This template ({{cite pmid}}) is deprecated. To cite the publication identified by PMID 20569424, please use {{cite journal}} with |pmid= 20569424 instead.
  17. ^ Template:Cite PMID

External links

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