The endometrial biopsy is a medical procedure that involves taking a tissue sample of the lining of the uterus. The tissue subsequently undergoes a histologic evaluation which aids the physician in forming a diagnosis.
There are a number of indications for obtaining an endometrial biopsy in a non-pregnant woman:
- Women with chronic anovulation such as the polycystic ovary syndrome are at increased risk for endometrial problems and an endometrial biopsy may be useful to assess their lining specifically to rule out endometrial hyperplasia or cancer.
- In women with abnormal vaginal bleeding the biopsy may indicate the presence of abnormal lining such as endometrial hyperplasia or cancer.
- In patients with suspected uterine cancer, the biopsy may discover the presence of cancer cells in the endometrium or cervix.
- In female infertility the assessment of the lining can determine, if properly timed, that the patient ovulated, however, the same information can be obtained by a blood test of the progesterone level.
Transvaginal ultrasonography is generally done before obtaining an endometrial biopsy as it may help in the gynecologic diagnosis, or even make the taking of a biospy superfluous if the lining is thin. If the endometrial lining is less than 5 mm thick on sonography, it is highly unusual to encounter endometrial cancer.
The procedure is contraindicated in pregnancy. Therefore, women in the reproductive years may need a pregnancy test before a biopsy is taken to assure that the test is not done during a pregnancy. Other contraindications are pelvic inflammatory disease and coagulopathies.
An endometrial biopsy usually cannot be done as an office procedure in children, young women, women with vaginismus, or women with cervical stenosis. If necessary, an examination under anesthesia could be performed at which time a biopsy could be taken.
Biopsy instruments 
A number of biopsy instruments are in use. The Novak curette is a thin metallic tube with a side opening at the tip; suction with attached syringe can be applied to help to remove tissue. The Pipelle is a more flexible plastic tube with a side opening at the tip. A smaller tube (internal piston) inside the Pipelle will be withdrawn to create suction. The pipelle will be rotated and moved in and out to collect small pieces of endometrial tissue. Recently, the TruTest has been introduced as an alternative method of endometrial biopsy. Rather than using a suction tube, this method uses the Tao Brush to gently brush the lining of the uterus. Generally, this method has been found to be less painful than the traditional suction method.
While procedure is generally considered safe, cramps or pelvic pain is a common if short-lived side effect. After the procedure, the patient may experience some bleeding. A uterine perforation or an infection are rare complications.
Endometrial Biopsy Pain 
Endometrial biopsy pain  is quite common. The reason that doctors spray the lidocain is to help to deal with some of the pain from the procedure. The lidocain spray can burn when it is sprayed into the area. The clamp that is put onto the cervix may be another cause of pain and the procedure itself, where the doctor has to procure a piece of the lining with the tube can be painful. Most of the time, this will only be required once, but sometimes the procedure can entain two or three instances of procuring a piece of the endometrium.
Endometrial biopsy procedure 
Generally, an endometrial biopsy follows this process:
3. A tenaculum, a type of forceps, will hold the cervix steady for the biopsy.
4. The biopsy curette will be inserted into the uterine fundus and with a scraping and rotating motion some tissue will be removed.
5. The removed tissue will be placed in formalin or equivalent for preservation.
Both the application of the tenaculum as well as the removal of tissue by the biopsy may cause pain. Patients, in general, may want to take some pain medication (such as ibuprofen) before the procedure and inquire about local anesthesia.
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- "Endometrial biopsy". The New York Times. Retrieved March 11, 2010.
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-  St. Joseph’s Mercy Health Center – Diagnostic and Surgical Tests and Procedures – Endometrial Biopsy (accessed 7-10-07)
- : Truth about Endometrial Biopsy Procedure Pain