The brewery had a capacity of 2,200 bbl per year. The operation closed on March 22, 2002. The former location of the Holy Cow briefly served as a sales office for the now cancelled Ivana condo project. The building was demolished in late April 2012.
At one point, Harry Caray, the announcer of the Chicago Cubs who was known for his saying "Holy cow!" during broadcasts, visited the Holy Cow Casino and Brewery and autographed a wall of the club with his signature saying.