Lemna

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Lemna
Kleine Wasserlinse (Entengrütze).JPG
Common Duckweed (Lemna minor)
Scientific classification
Kingdom: Plantae
(unranked): Angiosperms
(unranked): Monocots
Order: Alismatales
Family: Araceae
Subfamily: Lemnoideae
Tribe: Lemneae
Genus: Lemna
L.
Synonyms[1][2]
  • Staurogeton Rchb.
  • Lenticularia Ség.
  • Lenticula P.Micheli ex Adans.
  • Hydrophace Hallier
  • Telmatophace Schleid.
  • Thelmatophace Godr.
  • Lenticularia P.Micheli ex Montandon

Lemna is a genus of free-floating aquatic plants from the duckweed family. These rapidly growing plants have found uses as a model system for studies in community ecology, basic plant biology, in ecotoxicology, in production of biopharmaceuticals, and as a source of animal feeds for agriculture and aquaculture.

Taxonomy and growth habits[edit]

The duckweeds have been classified as a separate family, the Lemnaceae, but some researchers (the AGP II) consider the duckweeds members of the Araceae.

Lemna species grow as simple free-floating thalli on or just beneath the water surface. Most are small, not exceeding 5 mm in length, except Lemna trisulca which is elongated and has a branched structure. Lemna thalli have a single root, which distinguishes them from related genera Spirodela and Landoltia

The plants grow mainly by vegetative reproduction: two daughter plants bud off from the adult plant. This form of growth allows very rapid colonisation of new water. Duckweeds are flowering plants, and nearly all of them are known to reproduce sexually, flowering and producing seed under appropriate conditions. Certain duckweeds (e.g. L. gibba) are long day plants, while others (e.g. L. minor) are short day plants.

When Lemna invades a waterway, it can be removed mechanically, by the addition of herbivorous fish (e.g. grass carp) or treated with a herbicide.

The rapid growth of duckweeds finds application in bioremediation of polluted waters and as test organisms for environmental studies. It is also being used as an expression system for economical production of complex biopharmaceuticals.

Duckweed meal (dried duckweed) is a good cattle feed. It contains 25-45% proteins (depending on the growth conditions), 4.4% fat, and 8-10% fibre, measured by dry weight.

As a bioassay[edit]

Organisation for Economic Co-operation and Development[3] and U.S. Environmental Protection Agency (US EPA)[4] guidelines describe toxicity testing using Lemna gibba or Lemna minor as test organisms. Both of these species have been studied extensively for use in phytotoxicity tests. Genetic variability in responses to toxicants can occur in Lemna, and there are insufficient data to recommend a specific clone for testing. The US EPA test uses aseptic technique. The OECD test is not conducted axenically, but steps are taken at stages during the test procedure to keep contamination by other organisms to a minimum. Depending on the objectives of the test and the regulatory requirements, testing may be performed with renewal (semi-static and flow-through) or without renewal (static) of the test solution. Renewal is useful for substances that are rapidly lost from solution as a result of volatilisation, photodegradation, precipitation or biodegradation.

Production of biopharmaceuticals[edit]

Lemna has been transformed by molecular biologists to express proteins of pharmaceutical interest. Expression constructs were engineered to cause Lemna to secrete the transformed proteins into the growth medium at high yield. Since the Lemna is grown on a simple medium, this substantially reduces the burden of protein purification in preparing such proteins for medical use, promising substantial reductions in manufacturing costs.[5][6] In addition, the host Lemna can be engineered to cause secretion of proteins with human patterns of glycosylation, an improvement over conventional plant gene-expression systems.[7] Several such products are being developed, including monoclonal antibodies.

Duckweed farming[edit]

High yields of duckweed with a high protein content can be achieved by careful control of growth conditions. Although duckweed can tolerate temperatures ranging from 6 to 33 °C, the optimal growth range is 20 to 28 °C. The acceptable pH range is 5 to 9, but better growth is obtained in the pH range of 6.5 to 7.5. A minimum water depth of 1 ft is desirable to prevent excessive temperature swings. High nitrogen levels, for example 20 mM urea, have provided a protein content in the range of 45% by weight. The water may typically contain 60 mg/L of soluble nitrogen and 1 mg/L of phosphorus. Fertiliser is required on a daily basis for optimal growth.

Duckweed can be farmed organically, with nutrients being supplied from a variety of sources, for example cattle dung, pig waste, biogas plant slurry, or other organic matter in slurry form. Because of the rapid growth of duckweed, daily harvesting is necessary to achieve optimal yields. Harvesting is done such that less than a kilogram per square metre of duckweed remains. Under optimal conditions, a duckweed farm can produce 10 to 30 tons of dried duckweed per hectare per year.[8]

Selected species[edit]

Section Lemna
Section Uninerves
unknown section

LIAM *Lemna japonica Landolt - Japan, China, Korea, Russian Far East

Formerly placed here[edit]

References[edit]

  1. ^ Kew World Checklist of Selected Plant Families
  2. ^ "Genus: Lemna L.". Germplasm Resources Information Network. United States Department of Agriculture. 2006-11-03. Retrieved 2013-04-13. 
  3. ^ SourceOECD: issues
  4. ^ http://www.epa.gov/opptsfrs/publications/OPPTS_Harmonized/850_Ecological_Effects_Test_Guidelines/Drafts/850-4400.pdf
  5. ^ "Biolex Corporate Website". 
  6. ^ Gasdaska, JR; Spencer D and Dickey L (Mar–Apr 2003). "Advantages of Therapeutic Protein Production in the Aquatic Plant Lemna". BioProcessing Journal: 49–56. 
  7. ^ Cox, KM; Sterling JD, Regan JT, Gasdaska JR, Frantz KK, Peele CG, Black A, Passmore D, Moldovan-Loomis C, Srinivasan M, Cuison S, Cardarelli PM and Dickey LF (December 2006). "Glycan Optimization of a Human Monoclonal Antibody in the Aquatic Plant Lemna Minor". Nature Biotechnology 24 (12): 1591–1597. doi:10.1038/nbt1260. PMID 17128273. 
  8. ^ Leng, R A; J H Stambolie and R Bell (October 1995). "Duckweed - a potential high-protein feed resource for domestic animals and fish" ([dead link]Scholar search). Livestock Research for Rural Development 7 (1). 
  9. ^ "Lemna". Integrated Taxonomic Information System. Retrieved 2013-04-13. 
  10. ^ a b "GRIN Species Records of Lemna". Germplasm Resources Information Network. United States Department of Agriculture. Retrieved 2013-04-13. 

General readings[edit]

  • Cross, J.W. (2006). The Charms of Duckweed.
  • Landolt, E. (1986) Biosystematic investigations in the family of duckweeds (Lemnaceae). Vol. 2. The family of Lemnaceae - A monographic study. Part 1 of the monograph: Morphology; karyology; ecology; geographic distribution; systematic position; nomenclature; descriptions. Veröff. Geobot. Inst., Stiftung Rübel, ETH, Zurich.

External links[edit]