SEPT9: Difference between revisions
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==Clinical Significance== |
==Clinical Significance== |
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The v2 region of the SEPT9 promoter has been shown to be [[methylated]] in [[colorectal cancer]] tissue compared with normal colonic mucosa.<ref>http://www.transatlantic-symposium.de/content/_docs/doc1241003872263.pdf</ref> Using highly sensitive real time PCR assays, methylated SEPT9 was detected in the blood of colorectal cancer patients. This alternate methylation pattern in cancer samples is suggestive of an aberrant activation or repression of the gene compared to normal tissue samples.<ref>{{cite journal |last=Grutzmann |first=Robert |authorlink= |coauthors= Molnar B, Pilarsky C, Habermann JK, Schlag PM, Saeger HD, Miehlke S, Stolz T, Model F, Roblick UJ, Bruch HP, Koch R, Liebenberg V, Devos T, Song X, Day RH, Sledziewski AZ, Lofton-Day C.|year=2008|month=November |title= Sensitive Detection of Colorectal Cancer in Peripheral Blood by Septin 9 DNA Methylation Assay|journal=PLoS ONE |volume=3 |issue=11 |pages=1–8 | issn = 1059-1524| pmid = 19018278 |doi = 10.1371/journal.pone.0003759 |pmc=2582436 |editor1-last=Najbauer |editor1-first=Joseph }}</ref><ref>{{cite journal| author= deVos T, Tetzner R, Model F, Weiss G, Schuster M, Distler J, Steiger KV, Grützmann R, Pilarsky C, Habermann JK, Fleshner PR, Oubre BM, Day R, Sledziewski AZ, Lofton-Day C.| title= Circulating methylated SEPT9 DNA in plasma is a biomarker for colorectal cancer|year=2009 |journal= Clin Chem. |volume = 55|issue=7 | pages = 1337–1346|pmid= 19406918 | doi=10.1373/clinchem.2008.115808}}</ref> |
The v2 region of the SEPT9 promoter has been shown to be [[methylated]] in [[colorectal cancer]] tissue compared with normal colonic mucosa.<ref>http://www.transatlantic-symposium.de/content/_docs/doc1241003872263.pdf</ref> Using highly sensitive real time PCR assays, methylated SEPT9 was detected in the blood of colorectal cancer patients. This alternate methylation pattern in cancer samples is suggestive of an aberrant activation or repression of the gene compared to normal tissue samples.<ref>{{cite journal |last=Grutzmann |first=Robert |authorlink= |coauthors= Molnar B, Pilarsky C, Habermann JK, Schlag PM, Saeger HD, Miehlke S, Stolz T, Model F, Roblick UJ, Bruch HP, Koch R, Liebenberg V, Devos T, Song X, Day RH, Sledziewski AZ, Lofton-Day C.|year=2008|month=November |title= Sensitive Detection of Colorectal Cancer in Peripheral Blood by Septin 9 DNA Methylation Assay|journal=PLoS ONE |volume=3 |issue=11 |pages=1–8 | issn = 1059-1524| pmid = 19018278 |doi = 10.1371/journal.pone.0003759 |pmc=2582436 |editor1-last=Najbauer |editor1-first=Joseph }}</ref><ref>{{cite journal| author= deVos T, Tetzner R, Model F, Weiss G, Schuster M, Distler J, Steiger KV, Grützmann R, Pilarsky C, Habermann JK, Fleshner PR, Oubre BM, Day R, Sledziewski AZ, Lofton-Day C.| title= Circulating methylated SEPT9 DNA in plasma is a biomarker for colorectal cancer|year=2009 |journal= Clin Chem. |volume = 55|issue=7 | pages = 1337–1346|pmid= 19406918 | doi=10.1373/clinchem.2008.115808}}</ref> |
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Testing to detect methylated SEPT9 is not indicated as a first option for colorectal cancer screening.<ref name="ASCPfive">{{Citation |author1 = American Society for Clinical Pathology |author1-link = American Society for Clinical Pathology |date = |title = Five Things Physicians and Patients Should Question |publisher = American Society for Clinical Pathology |work = [[Choosing Wisely]]: an initiative of the [[ABIM Foundation]] |page = |url = http://www.choosingwisely.org/doctor-patient-lists/american-society-for-clinical-pathology/ |accessdate = August 1, 2013}}, which cites |
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#*{{cite doi|10.1053/j.gastro.2010.05.068}} |
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#*{{cite PMID|22019796}}</ref> It is similar in specificity and sensitivity to the [[stool guaiac test]] or fecal immune tests, and those tests should be used in preference.<ref name="ASCPfive"/> In cases when the physician aggressively has recommended a [[colonoscopy]] and the patient has declined that and these other tests, then this test has advantages over patients having no screening at all.<ref name="ASCPfive"/> |
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==See also== |
==See also== |
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Revision as of 18:34, 23 October 2013
Template:PBB Septin-9 is a protein that in humans is encoded by the SEPT9 gene.[1][2][3]
Interactions
SEPT9 has been shown to interact with SEPT2[4] and SEPT7.[4]
Biology
Along with AHNAK, eIF4E and S100A11, SEPT9 has been shown to be essential for pseudopod protrusion, tumor cell migration and invasion.[5]
Clinical Significance
The v2 region of the SEPT9 promoter has been shown to be methylated in colorectal cancer tissue compared with normal colonic mucosa.[6] Using highly sensitive real time PCR assays, methylated SEPT9 was detected in the blood of colorectal cancer patients. This alternate methylation pattern in cancer samples is suggestive of an aberrant activation or repression of the gene compared to normal tissue samples.[7][8]
Testing to detect methylated SEPT9 is not indicated as a first option for colorectal cancer screening.[9] It is similar in specificity and sensitivity to the stool guaiac test or fecal immune tests, and those tests should be used in preference.[9] In cases when the physician aggressively has recommended a colonoscopy and the patient has declined that and these other tests, then this test has advantages over patients having no screening at all.[9]
See also
References
- ^ Osaka M, Rowley JD, Zeleznik-Le NJ (1999). "MSF (MLL septin-like fusion), a fusion partner gene of MLL, in a therapy-related acute myeloid leukemia with a t(11;17)(q23;q25)". Proc Natl Acad Sci U S A. 96 (11): 6428–33. doi:10.1073/pnas.96.11.6428. PMC 26898. PMID 10339604.
{{cite journal}}: Unknown parameter|month=ignored (help)CS1 maint: multiple names: authors list (link) - ^ Taki T, Ohnishi H, Shinohara K, Sako M, Bessho F, Yanagisawa M, Hayashi Y (1999). "AF17q25, a putative septin family gene, fuses the MLL gene in acute myeloid leukemia with t(11;17)(q23;q25)". Cancer Res. 59 (17): 4261–5. PMID 10485469.
{{cite journal}}: Unknown parameter|month=ignored (help)CS1 maint: multiple names: authors list (link) - ^ "Entrez Gene: SEPT9 septin 9".
- ^ a b Surka, Mark C (2002). "The Mammalian Septin MSF Localizes with Microtubules and Is Required for Completion of Cytokinesis". Mol. Biol. Cell. 13 (10). United States: 3532–45. doi:10.1091/mbc.E02-01-0042. ISSN 1059-1524. PMC 129964. PMID 12388755.
{{cite journal}}: Cite has empty unknown parameters:|laydate=,|laysummary=, and|laysource=(help); Unknown parameter|coauthors=ignored (|author=suggested) (help); Unknown parameter|month=ignored (help) - ^ Shankar, Jay (2010). "Pseudopodial actin dynamics control epithelial-mesenchymal transition in metastatic cancer cells". Cancer Res. 70 (9): 3780–90. doi:10.1158/0008-5472.CAN-09-4439. PMID 20388789.
{{cite journal}}: Unknown parameter|coauthors=ignored (|author=suggested) (help); Unknown parameter|month=ignored (help) - ^ http://www.transatlantic-symposium.de/content/_docs/doc1241003872263.pdf
- ^ Grutzmann, Robert (2008). Najbauer, Joseph (ed.). "Sensitive Detection of Colorectal Cancer in Peripheral Blood by Septin 9 DNA Methylation Assay". PLoS ONE. 3 (11): 1–8. doi:10.1371/journal.pone.0003759. ISSN 1059-1524. PMC 2582436. PMID 19018278.
{{cite journal}}: Unknown parameter|coauthors=ignored (|author=suggested) (help); Unknown parameter|month=ignored (help)CS1 maint: unflagged free DOI (link) - ^ deVos T, Tetzner R, Model F, Weiss G, Schuster M, Distler J, Steiger KV, Grützmann R, Pilarsky C, Habermann JK, Fleshner PR, Oubre BM, Day R, Sledziewski AZ, Lofton-Day C. (2009). "Circulating methylated SEPT9 DNA in plasma is a biomarker for colorectal cancer". Clin Chem. 55 (7): 1337–1346. doi:10.1373/clinchem.2008.115808. PMID 19406918.
{{cite journal}}: CS1 maint: multiple names: authors list (link) - ^ a b c American Society for Clinical Pathology, "Five Things Physicians and Patients Should Question", Choosing Wisely: an initiative of the ABIM Foundation, American Society for Clinical Pathology, retrieved August 1, 2013, which cites
- Attention: This template ({{cite doi}}) is deprecated. To cite the publication identified by doi:10.1053/j.gastro.2010.05.068, please use {{cite journal}} (if it was published in a bona fide academic journal, otherwise {{cite report}} with
|doi=10.1053/j.gastro.2010.05.068instead. - Template:Cite PMID
- Attention: This template ({{cite doi}}) is deprecated. To cite the publication identified by doi:10.1053/j.gastro.2010.05.068, please use {{cite journal}} (if it was published in a bona fide academic journal, otherwise {{cite report}} with
Further reading
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