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transcribed using oligo-dT primers that are the reverse complement of the poly-adenylated tail on the 3' end of all mRNA. The oligo-dT primer anneals to...19 KB (2,275 words) - 13:01, 9 May 2024
reverse transcription primers able to capture mRNA with the oligo(dT) tails. Besides oligo(dT) tail and spatial barcode, which indicates the x and y position...55 KB (6,350 words) - 07:09, 19 May 2024- desired mRNA bound to the column is then eluted. Once mRNA is purified, an oligo-dT primer (a short sequence of deoxy-thymidine nucleotides) is bound to the...7 KB (891 words) - 15:13, 19 February 2024
PMID 6206782. Krug MS, Berger SL (1987). "First-strand cDNA synthesis primed with oligo(dT)". Guide to Molecular Cloning Techniques. Methods in Enzymology. Vol. 152...16 KB (1,400 words) - 23:34, 8 March 2024- method does not require the addition of nucleotides by TdT. cDNAs are generated using an Oligo-dT-adaptor primer (a primer with a short sequence of deoxy-thymine...8 KB (1,025 words) - 07:12, 11 March 2023
or from cells. Eukaryotic mRNA can then be isolated through the use of oligo (dT) cellulose chromatography to isolate only those RNAs with a poly(A) tail...17 KB (2,120 words) - 06:51, 23 March 2024- specialized oligo-dT (5’-biotin-triethyleneglycol-AAGCAGTGGTATCAACGCAGAGTAC(T)30VN-3’) conjugated to streptavidin-coupled paramagnetic beads. The oligo-dT binds...22 KB (2,613 words) - 16:08, 4 November 2023
- the RT is performed with a single-stranded sequence of deoxythymine (oligo dT) primer which bind specifically the poly(A) tail of mRNA molecules. Subsequently...74 KB (8,560 words) - 19:32, 14 May 2024
reverse transcription after the addition of unique optimized barcoded oligo(dT) primers. These primers uniquely tag the 3’ poly(A) tail of mRNA molecules...15 KB (1,751 words) - 19:43, 12 May 2024
Morpholino (redirect from Phosphorodiamidate morpholino oligo)Morpholino oligomer (PMO), is a type of oligomer molecule (colloquially, an oligo) used in molecular biology to modify gene expression. Its molecular structure...37 KB (4,376 words) - 04:22, 30 December 2023
Poly(p-phenylene vinylene) (redirect from Oligo(p-phenylenevinylene))Bibcode:1990Natur.347..539B. doi:10.1038/347539a0. S2CID 43158308. Moses D (June 1992). "High quantum efficiency luminescence from a conducting polymer...10 KB (1,115 words) - 17:17, 1 April 2024- (Shiraki et al., 2003) was using CAP Trapper for capturing the 5′ ends, oligo-dT primers for synthesizing the cDNAs, the type IIs restriction enzyme MmeI...11 KB (1,287 words) - 15:45, 5 March 2024
- FODMAP (redirect from Fermentable Oligo-, Di-, Monosaccharides And Polyols)IBS is debated. Lactose is a component of the FODMAPs (highly fermentable oligo-, di-, monosaccharides and polyols) diet. Several studies confirmed the...29 KB (3,265 words) - 03:47, 18 May 2024
are as follows: cDNA is reverse transcribed from mRNA using random or oligo dT primers. The cap trapper method is then employed to ensure selection of...79 KB (5,074 words) - 08:20, 15 February 2024
from primary infected tissue. Messenger RNA is then purified using an oligo-dT column that binds to the poly-A tail, a signal specifically found on transcribed...12 KB (1,428 words) - 07:38, 12 December 2023
R.M.D. Beck; J. Muirhead (2010). "An exceptionally well-preserved short-snouted bandicoot (Marsupialia; Peramelemorphia) from Riversleigh's Oligo-Miocene...19 KB (2,109 words) - 07:34, 2 March 2024
Ovulation (redirect from Oligo-ovulation)Oxford [Oxfordshire]: Oxford University Press. ISBN 978-0-19-920380-2. Baird, D. T.; Balen, A.; Escobar-Morreale, H. F.; Evers, J. L. H.; Fauser, B. C. J. M...29 KB (2,968 words) - 06:38, 28 April 2024- is similar to ClickSeq, however, the reverse-transcription step uses an oligo-dT primer (unanchored) to initiate cDNA synthesis from within the poly(A)...10 KB (1,160 words) - 22:52, 3 December 2023
Sucrase-isomaltase (redirect from Oligo-1,6-glucosidase)dependent on experimental conditions. ntSI’s four monomers, A, B, C, and D are included in the crystal asymmetric unit and have identical active sites...12 KB (1,494 words) - 22:58, 15 May 2024- 6G-fructosyltransferase (redirect from 1F-oligo(beta-D-fructofuranosyl-(2-1)-)sucrose 6G-beta-D-fructotransferase)The systematic name of this enzyme class is 1F-oligo[beta-D-fructofuranosyl-(2->1)-]sucrose 6G-beta-D-fructotransferase. Other names in common use include...3 KB (343 words) - 12:47, 26 August 2023
- report about three patients with right frontal intracerebral lesions, one oligo-astrocytoma and two vascular malformations, associated with partial seizures
- intercalations of marine beds. [Gr. oligos, little, kainos, new.] Oligochrome, ol′i-gō-krōm, adj. and n. painted in few colours. [Gr. oligos, few, chrōma, colour.]
- method of terminal transferase (TdT) and be blocked at the other 3' end .thus, the cDNA can be bind to the oligo-dT present at the surface of the cell