DNA condensation
DNA condensation refers to the process of compacting DNA molecules in vitro or in vivo.[1] Mechanistic details of DNA packing are essential for its functioning in the process of gene regulation in living systems. Condensed DNA often has surprising properties, which one would not predict from classical concepts of dilute solutions. Therefore DNA condensation in vitro serves as a model system for many processes of physics, biochemistry and biology.[2] In addition, DNA condensation has many potential applications in medicine and biotechnology.
DNA diameter is about 2 nm, while the length of a stretched single molecule may be up to several dozens of centimetres depending on the organism. Many features of the DNA double helix contribute to its large stiffness, including the mechanical properties of the sugar-phosphate backbone, electrostatic repulsion between phosphates (DNA bears on average one elementary negative charge per each 0.17 nm of the double helix), stacking interactions between the bases of each individual strand, and strand-strand interactions. DNA one of the stiffest natural polymers, yet it is also one of the longest molecules. This means that at large distances DNA can be considered as a flexible rope, and on a short scale as a stiff rod. Like a garden hose, unpacked DNA would randomly occupy a much larger volume than when it is orderly packed. Mathematically, for a non-interacting flexible chain randomly diffusing in 3D, the end-to-end distance would scale as a square root of the polymer length. For real polymers such as DNA this gives only very rough estimate; what is important, is that the space available for the DNA in vivo is much smaller than the space that it would occupy in the case of a free diffusion in the solution. In order to cope with the volume constraints, DNA has a striking property to pack itself in the appropriate solution conditions with the help of ions and other molecules. Usually, DNA condensation is defined as "the collapse of extended DNA chains into compact, orderly particles containing only one or a few molecules" [3]. This definition applies to many situations in vitro and is also close to the definition of DNA condensation in bacteria as "adoption of relatively concentrated, compact state occupying a fraction of the volume available"[4]. In eukaryotes, the DNA size and the number of other participating players are much larger, and a DNA molecule forms millions of ordered nucleoprotein particles, the nucleosomes, which is just the first of many levels of DNA packing.[1]
References
- ^ a b Teif V.B. and Bohinc K. (2010). "Condensed DNA: condensing the concepts". Progress in Biophysics and Molecular Biology. doi:10.1016/j.pbiomolbio.2010.07.002.
- ^ Bloomfield V.A. (1996). "DNA condensation". Current Opinion in Structural Biology.
{{cite journal}}: Unknown parameter|Issue=ignored (|issue=suggested) (help); Unknown parameter|Pages=ignored (|pages=suggested) (help) - ^ Bloomfield V.A. (1997). "DNA condensation by multivalent cations". Biopolymers. doi:10.1002/(SICI)1097-0282(1997)44:3<269::AID-BIP6>3.0.CO;2-T.
- ^ Zimmerman S.B. and Murphy L.D. (1996). "Macromolecular crowding and the mandatory condensation of DNA in bacteria". FEBS Letters. doi:10.1016/0014-5793(96)00725-9.
Bloomfield, V. A. 1996. DNA condensation. Curr Opin Struct Biol 6:334-341.
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