4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase
| 4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.7.1.17 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase (EC 3.7.1.17, tesD (gene), hsaD (gene)) is an enzyme with systematic name 4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase ( (2Z,4Z)-2-hydroxyhexa-2,4-dienoate-forming).[1][2][3][4] This enzyme catalyses the following chemical reaction
- (1E,2Z)-3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate + H2O 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate + (2Z,4Z)-2-hydroxyhexa-2,4-dienoate
The enzyme is involved in the bacterial degradation of the steroid ring structure.
References
[edit]- ^ Horinouchi M, Hayashi T, Koshino H, Kurita T, Kudo T (September 2005). "Identification of 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid, 4-hydroxy-2-oxohexanoic acid, and 2-hydroxyhexa-2,4-dienoic acid and related enzymes involved in testosterone degradation in Comamonas testosteroni TA441". Applied and Environmental Microbiology. 71 (9): 5275–81. Bibcode:2005ApEnM..71.5275H. doi:10.1128/aem.71.9.5275-5281.2005. PMC 1214608. PMID 16151114.
- ^ Van der Geize R, Yam K, Heuser T, Wilbrink MH, Hara H, Anderton MC, Sim E, Dijkhuizen L, Davies JE, Mohn WW, Eltis LD (February 2007). "A gene cluster encoding cholesterol catabolism in a soil actinomycete provides insight into Mycobacterium tuberculosis survival in macrophages". Proceedings of the National Academy of Sciences of the United States of America. 104 (6): 1947–52. Bibcode:2007PNAS..104.1947V. doi:10.1073/pnas.0605728104. PMC 1794314. PMID 17264217.
- ^ Lack N, Lowe ED, Liu J, Eltis LD, Noble ME, Sim E, Westwood IM (January 2008). "Structure of HsaD, a steroid-degrading hydrolase, from Mycobacterium tuberculosis". Acta Crystallographica Section F. 64 (Pt 1): 2–7. doi:10.1107/s1744309107065931. PMC 2373992. PMID 18097091.
- ^ Lack NA, Yam KC, Lowe ED, Horsman GP, Owen RL, Sim E, Eltis LD (January 2010). "Characterization of a carbon-carbon hydrolase from Mycobacterium tuberculosis involved in cholesterol metabolism". The Journal of Biological Chemistry. 285 (1): 434–43. doi:10.1074/jbc.m109.058081. PMC 2804191. PMID 19875455.
External links
[edit]- 4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate+hydrolase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
