FBXO31

FBXO31
Identifiers
Aliases	FBXO31, FBX14, FBXO14, Fbx31, pp2386, MRT45, F-box protein 31
External IDs	OMIM: 609102; MGI: 1354708; HomoloGene: 11684; GeneCards: FBXO31; OMA:FBXO31 - orthologs
Gene location (Human) Chr. Chromosome 16 (human) Band 16q24.2 Start 87,326,987 bp End 87,392,142 bp
Gene location (Mouse) Chr. Chromosome 8 (mouse) Band 8|8 E1 Start 122,276,179 bp End 122,305,545 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in cerebellar hemisphere right hemisphere of cerebellum right testis left testis cerebellar vermis gastrocnemius muscle muscle of thigh subcutaneous adipose tissue anterior pituitary popliteal artery Top expressed in muscle of thigh interventricular septum triceps brachii muscle temporal muscle digastric muscle superior frontal gyrus skeletal muscle tissue sternocleidomastoid muscle primary visual cortex thoracic diaphragm More reference expression data BioGPS n/a
Gene ontology Molecular function cyclin binding ubiquitin-protein transferase activity protein binding Cellular component SCF ubiquitin ligase complex cytosol soma centrosome Biological process cell cycle SCF-dependent proteasomal ubiquitin-dependent protein catabolic process mitotic G1 DNA damage checkpoint signaling protein ubiquitination cellular response to DNA damage stimulus protein polyubiquitination anaphase-promoting complex-dependent catabolic process post-translational protein modification positive regulation of dendrite morphogenesis positive regulation of neuron migration Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 79791 76454 Ensembl ENSG00000103264 ENSMUSG00000052934 UniProt Q5XUX0 Q3TQF0 RefSeq (mRNA) NM_024735 NM_001282683 NM_133765 RefSeq (protein) NP_001269612 NP_079011 NP_598526 Location (UCSC) Chr 16: 87.33 – 87.39 Mb Chr 8: 122.28 – 122.31 Mb PubMed search
Wikidata
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F-box only protein 31 is a protein that in humans is encoded by the FBXO31 gene.

Function

Members of the F-box protein family, such as FBXO31, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains.

F-box protein FBXO31 directs degradation of MDM2 to facilitate p53-mediated growth arrest following genotoxic stress. the F-box protein FBXO31, a candidate tumor suppressor encoded in 16q24.3 for which there is loss of heterozygosity in various solid tumors, is responsible for promoting MDM2 degradation. Following genotoxic stress, FBXO31 is phosphorylated by the DNA damage serine/threonine kinase ATM, resulting in increased levels of FBXO31. FBXO31 then interacts with and directs the degradation of MDM2, which is dependent on phosphorylation of MDM2 by ATM. FBXO31-mediated loss of MDM2 leads to elevated levels of p53, resulting in growth arrest. In cells depleted of FBXO31, MDM2 is not degraded and p53 levels do not increase following genotoxic stress. Thus, FBXO31 is essential for the classic robust increase in p53 levels following DNA damage.

Further reading

• Hartley JL, Temple GF, Brasch MA (November 2000). "DNA cloning using in vitro site-specific recombination". Genome Research. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
• Powell JA, Gardner AE, Bais AJ, Hinze SJ, Baker E, Whitmore S, Crawford J, Kochetkova M, Spendlove HE, Doggett NA, Sutherland GR, Callen DF, Kremmidiotis G (September 2002). "Sequencing, transcript identification, and quantitative gene expression profiling in the breast cancer loss of heterozygosity region 16q24.3 reveal three potential tumor-suppressor genes". Genomics. 80 (3): 303–10. doi:10.1006/geno.2002.6828. PMID 12213200.
• Wiemann S, Arlt D, Huber W, Wellenreuther R, Schleeger S, Mehrle A, Bechtel S, Sauermann M, Korf U, Pepperkok R, Sültmann H, Poustka A (October 2004). "From ORFeome to biology: a functional genomics pipeline". Genome Research. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
• Kumar R, Neilsen PM, Crawford J, McKirdy R, Lee J, Powell JA, Saif Z, Martin JM, Lombaerts M, Cornelisse CJ, Cleton-Jansen AM, Callen DF (December 2005). "FBXO31 is the chromosome 16q24.3 senescence gene, a candidate breast tumor suppressor, and a component of an SCF complex". Cancer Research. 65 (24): 11304–13. doi:10.1158/0008-5472.CAN-05-0936. PMID 16357137.
• Mehrle A, Rosenfelder H, Schupp I, del Val C, Arlt D, Hahne F, Bechtel S, Simpson J, Hofmann O, Hide W, Glatting KH, Huber W, Pepperkok R, Poustka A, Wiemann S (January 2006). "The LIFEdb database in 2006". Nucleic Acids Research. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.