Vesicle-associated membrane protein 8

VAMP8
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	4WY4
Identifiers
Aliases	VAMP8, EDB, VAMP-8, vesicle associated membrane protein 8
External IDs	OMIM: 603177; MGI: 1336882; HomoloGene: 37846; GeneCards: VAMP8; OMA:VAMP8 - orthologs
Gene location (Human)
Chr.	Chromosome 2 (human)
End	85,582,031 bp
Gene location (Mouse)
Chr.	Chromosome 6 (mouse)
End	72,367,686 bp
RNA expression pattern
	Top expressed in
	palpebral conjunctiva; ; monocyte; ; mucosa of transverse colon; ; olfactory zone of nasal mucosa; ; granulocyte; ; jejunal mucosa; ; rectum; ; nasal epithelium; ; bronchial epithelial cell; ; skin of abdomen;
	Top expressed in
	proximal tubule; ; right kidney; ; yolk sac; ; granulocyte; ; lung; ; bone marrow; ; human kidney; ; urinary bladder; ; duodenum; ; lip;
	More reference expression data
	More reference expression data
Gene ontology
Molecular function	SNAP receptor activity; protein binding; chloride channel inhibitor activity; SNARE binding;
Cellular component	cytoplasm; integral component of membrane; recycling endosome; vesicle; cytosol; endosome; early endosome membrane; membrane; secretory granule membrane; late endosome membrane; plasma membrane; mucin granule; lysosomal membrane; SNARE complex; early endosome; perinuclear region of cytoplasm; lysosome; extracellular exosome; clathrin-coated vesicle membrane; phagocytic vesicle membrane; azurophil granule membrane; specific granule membrane; recycling endosome membrane; tertiary granule membrane; synaptic vesicle;
Biological process	post-Golgi vesicle-mediated transport; autophagy; autophagosome maturation; negative regulation of secretion by cell; protein transport; viral entry into host cell; vesicle fusion; eosinophil degranulation; vesicle-mediated transport; mucus secretion; regulation of protein localization to plasma membrane; positive regulation of histamine secretion by mast cell; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; autophagosome membrane docking; neutrophil degranulation; membrane organization; regulation of molecular function; transport;
	Sources:Amigo / QuickGO
Orthologs
	8673
	22320
	ENSG00000118640
	ENSMUSG00000050732
	Q9BV40
	O70404
	NM_003761
	NM_016794
	NP_003752
	NP_058074
	Wikidata
View/Edit Human	View/Edit Mouse

Vesicle-associated membrane protein 8 is a protein that in humans is encoded by the VAMP8 gene.^[5]^[6]^[7]

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. It is associated with the perinuclear vesicular structures of the early endocytic compartment. It has been found that VAMP8 interacts specifically with the soluble NSF-attachment protein (alpha-SNAP), most likely through an VAMP8-containing SNARE complex.^[7] Phosphorylation of VAMP8 inside the conserved SNARE-domain can suppress vesicle fusion.^[8]

Interactions

Vesicle-associated membrane protein 8 has been shown to interact with STX4,^[9]^[10] SNAP23,^[9]^[11] STX1A,^[12] STX8^[13] and STX7.^[13]^[14]

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000118640 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000050732 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Bui TD, Wong SH, Lu L, Hong W (February 1999). "Endobrevin maps to chromosome 2 in human and chromosome 6 in mouse". Genomics. 54 (3): 579–80. doi:10.1006/geno.1998.5596. PMID 9878266.
^ Wong SH, Zhang T, Xu Y, Subramaniam VN, Griffiths G, Hong W (July 1998). "Endobrevin, a Novel Synaptobrevin/VAMP-Like Protein Preferentially Associated with the Early Endosome". Mol Biol Cell. 9 (6): 1549–63. doi:10.1091/mbc.9.6.1549. PMC 25382. PMID 9614193.
^ ^a ^b "Entrez Gene: VAMP8 vesicle-associated membrane protein 8 (endobrevin)".
^ Malmersjö S, Di Palma S, Diao J, Lai Y, Pfuetzner RA, Wang AL, McMahon MA, Hayer A, Porteus M, Bodenmiller B, Brunger AT, Meyer T (July 2016). "Phosphorylation of residues inside the SNARE complex suppresses secretory vesicle fusion". EMBO Journal. 35 (16): 1721–1843. doi:10.15252/embj.201694071. PMC 5010044. PMID 27402227.
^ ^a ^b Paumet, F; Le Mao J; Martin S; Galli T; David B; Blank U; Roa M (June 2000). "Soluble NSF attachment protein receptors (SNAREs) in RBL-2H3 mast cells: functional role of syntaxin 4 in exocytosis and identification of a vesicle-associated membrane protein 8-containing secretory compartment". J. Immunol. 164 (11): 5850–7. doi:10.4049/jimmunol.164.11.5850. ISSN 0022-1767. PMID 10820264.
^ Polgár, János; Chung Sul-Hee; Reed Guy L (August 2002). "Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion". Blood. 100 (3): 1081–3. doi:10.1182/blood.V100.3.1081. ISSN 0006-4971. PMID 12130530.
^ Imai, Akane; Nashida Tomoko; Yoshie Sumio; Shimomura Hiromi (August 2003). "Intracellular localisation of SNARE proteins in rat parotid acinar cells: SNARE complexes on the apical plasma membrane". Arch. Oral Biol. 48 (8): 597–604. doi:10.1016/S0003-9969(03)00116-X. ISSN 0003-9969. PMID 12828989.
^ Nagamatsu, S; Nakamichi Y; Watanabe T; Matsushima S; Yamaguchi S; Ni J; Itagaki E; Ishida H (January 2001). "Localization of cellubrevin-related peptide, endobrevin, in the early endosome in pancreatic beta cells and its physiological function in exo-endocytosis of secretory granules". J. Cell Sci. 114 (Pt 1): 219–227. ISSN 0021-9533. PMID 11112705.
^ ^a ^b Antonin, W; Holroyd C; Fasshauer D; Pabst S; Von Mollard G F; Jahn R (Dec 2000). "A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function". EMBO J. 19 (23): 6453–64. doi:10.1093/emboj/19.23.6453. ISSN 0261-4189. PMC 305878. PMID 11101518.
^ Wade, N; Bryant N J; Connolly L M; Simpson R J; Luzio J P; Piper R C; James D E (June 2001). "Syntaxin 7 complexes with mouse Vps10p tail interactor 1b, syntaxin 6, vesicle-associated membrane protein (VAMP)8, and VAMP7 in b16 melanoma cells". J. Biol. Chem. 276 (23): 19820–7. doi:10.1074/jbc.M010838200. ISSN 0021-9258. PMID 11278762.

Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
Fasshauer D; Antonin W; Margittai M; et al. (1999). "Mixed and non-cognate SNARE complexes. Characterization of assembly and biophysical properties". J. Biol. Chem. 274 (22): 15440–6. doi:10.1074/jbc.274.22.15440. PMID 10336434. {{cite journal}}: Unknown parameter |name-list-format= ignored (|name-list-style= suggested) (help)
Kim PK; Hollerbach C; Trimble WS; et al. (2000). "Identification of the endoplasmic reticulum targeting signal in vesicle-associated membrane proteins". J. Biol. Chem. 274 (52): 36876–82. doi:10.1074/jbc.274.52.36876. PMID 10601239. {{cite journal}}: Unknown parameter |name-list-format= ignored (|name-list-style= suggested) (help)
Paumet F; Le Mao J; Martin S; et al. (2000). "Soluble NSF attachment protein receptors (SNAREs) in RBL-2H3 mast cells: functional role of syntaxin 4 in exocytosis and identification of a vesicle-associated membrane protein 8-containing secretory compartment". J. Immunol. 164 (11): 5850–7. doi:10.4049/jimmunol.164.11.5850. PMID 10820264. {{cite journal}}: Unknown parameter |name-list-format= ignored (|name-list-style= suggested) (help)
Antonin W; Holroyd C; Tikkanen R; et al. (2000). "The R-SNARE Endobrevin/VAMP-8 Mediates Homotypic Fusion of Early Endosomes and Late Endosomes". Mol. Biol. Cell. 11 (10): 3289–98. doi:10.1091/mbc.11.10.3289. PMC 14992. PMID 11029036. {{cite journal}}: Unknown parameter |name-list-format= ignored (|name-list-style= suggested) (help)
Antonin W; Holroyd C; Fasshauer D; et al. (2001). "A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function". EMBO J. 19 (23): 6453–64. doi:10.1093/emboj/19.23.6453. PMC 305878. PMID 11101518. {{cite journal}}: Unknown parameter |name-list-format= ignored (|name-list-style= suggested) (help)
Nagamatsu S; Nakamichi Y; Watanabe T; et al. (2001). "Localization of cellubrevin-related peptide, endobrevin, in the early endosome in pancreatic beta cells and its physiological function in exo-endocytosis of secretory granules". J. Cell Sci. 114 (Pt 1): 219–227. PMID 11112705. {{cite journal}}: Unknown parameter |name-list-format= ignored (|name-list-style= suggested) (help)
Wade N; Bryant NJ; Connolly LM; et al. (2001). "Syntaxin 7 complexes with mouse Vps10p tail interactor 1b, syntaxin 6, vesicle-associated membrane protein (VAMP)8, and VAMP7 in b16 melanoma cells". J. Biol. Chem. 276 (23): 19820–7. doi:10.1074/jbc.M010838200. PMID 11278762. {{cite journal}}: Unknown parameter |name-list-format= ignored (|name-list-style= suggested) (help)
Antonin W; Fasshauer D; Becker S; et al. (2002). "Crystal structure of the endosomal SNARE complex reveals common structural principles of all SNAREs". Nat. Struct. Biol. 9 (2): 107–11. doi:10.1038/nsb746. hdl:11858/00-001M-0000-0012-F459-F. PMID 11786915. {{cite journal}}: Unknown parameter |name-list-format= ignored (|name-list-style= suggested) (help)
Polgár J, Chung SH, Reed GL (2002). "Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion". Blood. 100 (3): 1081–3. doi:10.1182/blood.V100.3.1081. PMID 12130530.
Strausberg RL; Feingold EA; Grouse LH; et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. {{cite journal}}: Unknown parameter |name-list-format= ignored (|name-list-style= suggested) (help)
Imai A, Nashida T, Yoshie S, Shimomura H (2003). "Intracellular localisation of SNARE proteins in rat parotid acinar cells: SNARE complexes on the apical plasma membrane". Arch. Oral Biol. 48 (8): 597–604. doi:10.1016/S0003-9969(03)00116-X. PMID 12828989.
Gerhard DS; Wagner L; Feingold EA; et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. {{cite journal}}: Unknown parameter |name-list-format= ignored (|name-list-style= suggested) (help)
Hillier LW; Graves TA; Fulton RS; et al. (2005). "Generation and annotation of the DNA sequences of human chromosomes 2 and 4". Nature. 434 (7034): 724–31. Bibcode:2005Natur.434..724H. doi:10.1038/nature03466. PMID 15815621. {{cite journal}}: Unknown parameter |name-list-format= ignored (|name-list-style= suggested) (help)
Brinkman JF; Ottenheim CP; de Jong LA; et al. (2006). "VAMP5 and VAMP8 are most likely not involved in primary open-angle glaucoma". Mol. Vis. 11: 582–6. PMID 16110299. {{cite journal}}: Unknown parameter |name-list-format= ignored (|name-list-style= suggested) (help)
Oishi Y; Arakawa T; Tanimura A; et al. (2007). "Role of VAMP-2, VAMP-7, and VAMP-8 in constitutive exocytosis from HSY cells". Histochem. Cell Biol. 125 (3): 273–81. doi:10.1007/s00418-005-0068-y. PMID 16195891. {{cite journal}}: Unknown parameter |name-list-format= ignored (|name-list-style= suggested) (help)
Shiffman D; Rowland CM; Louie JZ; et al. (2006). "Gene variants of VAMP8 and HNRPUL1 are associated with early-onset myocardial infarction". Arterioscler. Thromb. Vasc. Biol. 26 (7): 1613–8. doi:10.1161/01.ATV.0000226543.77214.e4. PMID 16690874. {{cite journal}}: Unknown parameter |name-list-format= ignored (|name-list-style= suggested) (help)

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000118640 – Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000050732 – Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid9878266-5] Bui TD, Wong SH, Lu L, Hong W (February 1999). "Endobrevin maps to chromosome 2 in human and chromosome 6 in mouse". Genomics. 54 (3): 579–80. doi:10.1006/geno.1998.5596. PMID 9878266.

[pmid9614193-6] Wong SH, Zhang T, Xu Y, Subramaniam VN, Griffiths G, Hong W (July 1998). "Endobrevin, a Novel Synaptobrevin/VAMP-Like Protein Preferentially Associated with the Early Endosome". Mol Biol Cell. 9 (6): 1549–63. doi:10.1091/mbc.9.6.1549. PMC 25382. PMID 9614193.

[entrez-7] "Entrez Gene: VAMP8 vesicle-associated membrane protein 8 (endobrevin)".

[pmid27402227-8] Malmersjö S, Di Palma S, Diao J, Lai Y, Pfuetzner RA, Wang AL, McMahon MA, Hayer A, Porteus M, Bodenmiller B, Brunger AT, Meyer T (July 2016). "Phosphorylation of residues inside the SNARE complex suppresses secretory vesicle fusion". EMBO Journal. 35 (16): 1721–1843. doi:10.15252/embj.201694071. PMC 5010044. PMID 27402227.

[pmid10820264-9] Paumet, F; Le Mao J; Martin S; Galli T; David B; Blank U; Roa M (June 2000). "Soluble NSF attachment protein receptors (SNAREs) in RBL-2H3 mast cells: functional role of syntaxin 4 in exocytosis and identification of a vesicle-associated membrane protein 8-containing secretory compartment". J. Immunol. 164 (11): 5850–7. doi:10.4049/jimmunol.164.11.5850. ISSN 0022-1767. PMID 10820264.

[pmid12130530-10] Polgár, János; Chung Sul-Hee; Reed Guy L (August 2002). "Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion". Blood. 100 (3): 1081–3. doi:10.1182/blood.V100.3.1081. ISSN 0006-4971. PMID 12130530.

[pmid12828989-11] Imai, Akane; Nashida Tomoko; Yoshie Sumio; Shimomura Hiromi (August 2003). "Intracellular localisation of SNARE proteins in rat parotid acinar cells: SNARE complexes on the apical plasma membrane". Arch. Oral Biol. 48 (8): 597–604. doi:10.1016/S0003-9969(03)00116-X. ISSN 0003-9969. PMID 12828989.

[pmid11112705-12] Nagamatsu, S; Nakamichi Y; Watanabe T; Matsushima S; Yamaguchi S; Ni J; Itagaki E; Ishida H (January 2001). "Localization of cellubrevin-related peptide, endobrevin, in the early endosome in pancreatic beta cells and its physiological function in exo-endocytosis of secretory granules". J. Cell Sci. 114 (Pt 1): 219–227. ISSN 0021-9533. PMID 11112705.

[pmid11101518-13] Antonin, W; Holroyd C; Fasshauer D; Pabst S; Von Mollard G F; Jahn R (Dec 2000). "A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function". EMBO J. 19 (23): 6453–64. doi:10.1093/emboj/19.23.6453. ISSN 0261-4189. PMC 305878. PMID 11101518.

[pmid11278762-14] Wade, N; Bryant N J; Connolly L M; Simpson R J; Luzio J P; Piper R C; James D E (June 2001). "Syntaxin 7 complexes with mouse Vps10p tail interactor 1b, syntaxin 6, vesicle-associated membrane protein (VAMP)8, and VAMP7 in b16 melanoma cells". J. Biol. Chem. 276 (23): 19820–7. doi:10.1074/jbc.M010838200. ISSN 0021-9258. PMID 11278762.

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Interactions

References

Further reading