DGLUCY
UPF0317 protein C14orf159, mitochondrial is a protein that in humans is encoded by the C14orf159 gene (chromosome 14 open reading frame 159).[5]
Orthologs
 | This section possibly contains original research. (January 2011) |
The human gene, c14orf159 mRNA, is highly conserved in mammals and birds.[6] Orthologs gathered from BLAST and BLAT searches reveal that the human c14orf159 mRNA sequence is conserved with a sequence identity of 98% in chimpanzees, 88% in mice, and 81% in platypus and chicken.[7][8] The following table contains a list orthologs that were gathered from BLAST searches. Sequence alignments were performed using blastn to derive sequence identity, score, and E-values between the human c14orf159 variant 1 mRNA and its orthologs.
| Genus and species | Common name | NCBI accession number | Sequence length (bp) | Sequence identity | Score | E-value |
|---|---|---|---|---|---|---|
| Homo sapiens | Human | NM_001102366 | 3164 | 100% | 0 | |
| Pan troglodytes | Chimpanzee | XM_510121 | 2974 | 98% | 4281 | 0 |
| Mus musculus | Mouse | NM_145448 | 3231 | 88% | 495 | 0 |
| Ornithorhynchus anatinus | Platypus | XM_00154336.1 | 1962 | 81% | 217 | 0 |
| Gallus gallus | Chicken | XM_421319 | 3389 | 81 | 50 | 0 |
The protein that the human gene c14orf159 encodes has been found to be highly conserved among mammals, birds, amphibians, fish, tunicates, cnidarians, and echinoderms. However, no protein orthologs have been found in nematodes, arthropods, fungi, protists, plants, bacteria, or archea. Fungi and bacteria contain the DUF1445 conserved domain which is found in human c14orf159 and its orthologs. BLAST and BLAT searches have been utilized to find orthologs to the c14orf159 protein. The following table lists protein orthologs for the human protein with sequence identity, sequence similarity, scores, and E-values derived from blastp sequence comparisons.[9]
| Genus and species | Common name | NCBI accession number | Sequence length-amino acids | Sequence identity | Sequence similarity | Score | E-value |
|---|---|---|---|---|---|---|---|
| Homo sapiens | Human | NP_001095839.1 | 564 | 100% | 100% | 0 | |
| Pan troglodytes | Chimpanzee | XP_510121.2 | 724 | 557/621 (89%) | 561/621 (90%) | 1109 | 0 |
| Ailuropoda melanoleuca | Panda | EFB15996.1 | 585 | 413/585 (70%) | 461/585 (78%) | 824 | 0 |
| Rattus norvegicus | Rat | XP_343096.2 | 618 | 423/618 (68%) | 470/618 (76%) | 774 | 0 |
| Mus musculus | Mouse | NP_663423.2 | 617 | 414/623 (66%) | 468/621 (75%) | 796 | 0 |
| Equus caballus | Horse | XP_001916913.1 | 581 | 390/585 (66%) | 433/585 (74%) | 728 | 6E-115 |
| Ornithorhynchus anatinus | Platypus | XP_001514386.1 | 653 | 358/628 (57%) | 443/628 (70%) | 696 | 0 |
| Gallus gallus | Chicken | XP_421319.2 | 617 | 330/614 (53%) | 414/614 (67%) | 630 | 0 |
| Xenopus tropicalis | Western clawed frog | CAJ82045.1 | 616 | 302/611 (49%) | 399/611 (65%) | 582 | 1E-170 |
| Danio rerio | Zebrafish | AAI244131.1 | 621 | 284/607 (46%) | 386/607 (63%) | 530 | 6E-155 |
| Branchiostoma floridae | Lancelet | XP_002612376.1 | 615 | 237/611 (38%) | 334/611 (54%) | 397 | 6E-115 |
| Ciona intestinalis | Vase tunicate | XP_001173256 | 486 | 161/501 (32%) | 241/501 (48%) | 244 | 5E-69 |
| Strongylocentrotus purpuratus | California purple sea urchin | XP_782739.1 | 631 | 9/33 (27%) | 15/33 (45%) | 320 | 5E-87 |
| Nematostella vectensis | Starlet sea anemone | XP_001637867 | 529 | 134/501 (26%) | 211/501 (42%) | 120 | 1E-31 |
Post-translational modification
The protein product of the C14orf159 gene is predicted[5] and was found[10][11] to be translocated to mitochondrion.
Post-translational modifications are predicted for the protein c14orf159. All predicted sites in human c14orf159 were compared to orthologs using multiple sequence alignments to determine likelihood of modification.[12][13][14][15] [16]
Regulation
Estrogen receptor alpha, in the presence of estradiol, binds to the C14orf159 gene and likely regulates its expression.[17]
References
- ^ a b c GRCh38: Ensembl release 89: ENSG00000133943 – Ensembl, May 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000021185 – Ensembl, May 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ a b "Entrez Gene: C14orf159 chromosome 14 open reading frame 159".
- ^ BLAST. NCBI. accessed 19 April 2010. http://blast.ncbi.nlm.nih.gov/Blast.cgi
- ^ UCSC Genome Browser website, BLAT. accessed 10 April 2010.
- ^ BLAST. NCBI. accessed 19 April 2010.
- ^ Blastp. NCBI. http://blast.ncbi.nlm.nih.gov/Blast.cgi
- ^ Mehrle A, Rosenfelder H. "RZPD CloneID DKFZp686J0759". LifeDB: Database for Localization, Interaction, Functional assays and Expression of Proteins. German Cancer Research Center.
- ^ Wiemann S, Arlt D, Huber W, Wellenreuther R, Schleeger S, Mehrle A, Bechtel S, Sauermann M, Korf U, Pepperkok R, Sültmann H, Poustka A (October 2004). "From ORFeome to Biology: A Functional Genomics Pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
- ^ Prediction of glycosylation across the human proteome and the correlation to protein function. Gupta, R. and S. Brunak. Pacific Symposium on Biocomputing, 7:310-322, 2002 <http://www.cbs.dtu.dk/services/YinOYang/>.
- ^ Locating proteins in the cell using TargetP, SignalP, and related tools Olof Emanuelsson, Søren Brunak, Gunnar von Heijne, Henrik Nielsen Nature Protocols 2, 953-971 (2007) http://www.cbs.dtu.dk/services/SignalP/.
- ^ Scanning the available Dictyostelium discoideum proteome for O-linked GlcNAc glycosylation sites using neural networks. R. Gupta, E. Jung, A.A. Gooley, K.L. Williams, S. Brunak and J. Hansen. Glycobiology: 9(10):1009-22, 1999 http://www.cbs.dtu.dk/services/DictyOGlyc/.
- ^ Analysis and prediction of mammalian protein glycation. Morten Bo Johansen, Lars Kiemer and Søren Brunak Glycobiology, 16:844-853, 2006 http://www.cbs.dtu.dk/services/NetGlycate/.
- ^ Sulfinator. Expasy tools. 2010. http://expasy.org/tools/sulfinator/.
- ^ Creekmore AL, Ziegler YS, Bonéy JL, Nardulli AM (March 2007). "Estrogen receptor α regulates expression of the breast cancer 1 associated ring domain 1 (BARD1) gene through intronic DNA sequence". Mol. Cell. Endocrinol. 267 (1–2): 106–15. doi:10.1016/j.mce.2007.01.001. PMC 1933484. PMID 17275994.