Clostridium innocuum

From Wikipedia, the free encyclopedia
Jump to navigation Jump to search

Clostridium innocuum
Scientific classification
Cl. innocuum
Binomial name
"Clostridium" innocuum
Smith and King 1962

Clostridium innocuum is an anaerobic, non-motile, gram-positive bacterium that reproduces by sporulation.[1] While there are over 130 species of Clostridium, C. innocuum is the third most commonly isolated. Although it is not normally considered an aggressive human pathogen, it has been isolated in some disease processes. C. innocuum and other Clostridium line the oropharynx and gastrointestinal tract, and are considered normal gut flora.[2]

Characteristics and identification[edit]

Anaerobic gram-positive bacilli affecting human beings are generally divided into two distinct groups, those that form spores (Clostridium spp) and those that do not form spores. Within the spore-forming group of Clostridium species, some are very pathogenic or toxigenic (C. perfringens) while others are rarely pathogenic. Identification and differentiation between anaerobic gram-positive bacteria in a clinical laboratory can be a very difficult task.

C. innocuum forms white, glossy, raised colonies and exhibits a chartreuse fluorescence. It is a small, non-motile rod that does not swarm. C. innocuum falls into the saccharolytic, non-proteolytic group of Clostridium spp. and is negative for gelatin hydrolysis, lecithinase, lipase, indole, urea, and nitrate. It is positive for glucose fermentation, esculin and produces oval, terminal spores.[3]


Although Clostridium innocuum are often present and harmless in healthy people, they have been isolated in various infections and predominantly in patients that are immunocompromised as an opportunistic bacteria. In one case, C. innocuum was isolated in an infected hematoma adjacent to a transplanted kidney in a 38-year-old, Hepatitis C patient. Originally, the bacteria was misidentified as another Clostridium species but was eventually correctly identified. This led the authors to state that C. innocuum infection post transplant may be severely underestimated.[4] C. innocuum has also been implicated in cases of fatal bacteremia as reported in a retrospective study done by Newark Beth Israel Medical Center. Patients that succumbed to the infection (100% mortality) had underlying disease states (malignancy, diabetes or liver disease). While clostridial species only account for 2% of clinical bacteremia, C innocuum was among the three most commonly isolated bacteria in this study.[5] C. innocuum has also been isolated in patients with recurrent diarrhea who had prior C. difficile associated diarrhea.[6] One case of fatal bacterial endocarditis was also found in the literature. This is possibly the only confirmed case of C. innocuum endocarditis involving the pulmonary and tricuspid valves resulting in multiple emboli and death.[7]

Drug resistance[edit]

In a study that identified clostridial species using commercial identification kits, it was found that as many as 10% of C. innocuum isolates were somewhat resistant to clindamycin. Most strains were found to be only moderately susceptible to vancomycin (MIC at which 90% of strains are inhibited, 4 micrograms/mL).[8] In another study, where C innocuum was positively identified by gas-liquid chromatography, resistance to vancomycin was seen in all 28 strains isolated. All other clostridial species were at least 8 times more susceptible to vancomycin than C. innocuum, suggesting an intrinsic vancomycin resistance mechanism in C. innocuum.[9]


  1. ^ Smith, Louis D. S.; King, Elizabeth (April 1962). "Clostridium innocuum, sp. n., a spore-forming anaerobe isolated from human infections". Journal of Bacteriology. 83: 938–939. PMC 279386. PMID 13914326.
  2. ^ Crum-Cianflone MD, MPH, Nancy (June 2009). "Clostridium innocuum bacteremia in a patient with acquired immune deficiency syndrome". American Journal of the Medical Sciences. 337 (6): 480–482. doi:10.1097/MAJ.0b013e31819f1e95. PMC 2732570. PMID 19525665.
  3. ^ Isenburg, Henry (2007). Clinical Microbiology Procedures Handbook 2nd Edition (PDF). Washington, DC: ASM press. pp. 4.11–4.11.9. ISBN 978-1-55581-243-0.
  4. ^ Castiglioni, B; Guatam (2003). "Clostridium innocuum bacteremia secondary to infected hematoma with gas formation in a kidney transplant recipient". Transplant Infectious Disease. 5 (4): 199–202. doi:10.1111/j.1399-3062.2003.00037.x. PMID 14987206.
  5. ^ Shah, Monica; Bishburg (February 2009). "Epidemiology and outcomes of clostridial bacteremia at a tertiary-care institution". Scientific World Journal. 9: 144–148. doi:10.1100/tsw.2009.21. PMC 5823103. PMID 19252754.
  6. ^ Ackermann, G; Tang (July 2001). "Isolation of Clostridium innocuum from cases of recurrent diarrhea in patients with prior Clostridium difficile associated diarrhea and". Diagnostic Microbiology and Infectious Diseases. 40 (3): 103–106. doi:10.1016/s0732-8893(01)00259-0. PMID 11502376.
  7. ^ Cutrona, AF; Watanakunacorn (Nov 1995). "Clostridium innocuum endocarditis". Clinical Infectious Diseases. 21 (5): 1306–1307. doi:10.1093/clinids/21.5.1306. PMID 8589163.
  8. ^ Alexander, CJ; Citron (Dec 1995). "Identification and antimicrobial resistance patterns of clinical isolates of Clostridium clostridioforme, Clostridium innocuum, and Clostridium ramosum compared with those of clinical isolates of Clostridium perfringens". Journal of Clinical Microbiology. 33 (12): 3209–3215. PMC 228675. PMID 8586704.
  9. ^ Mory, Francine; Lozneiwski (June 1998). "Low-Level Vancomycin Resistance in Clostridium innocuum". Journal of Clin Microbiology. 36 (6): 1767–1768. PMC 104917. PMID 9620417.

External links[edit]