Hfq binding sRNA

isrA Hfq binding RNA
isrA Hfq binding RNA
	Predicted secondary structure of isrA Hfq binding RNA
Identifiers
Symbol	isrA
Rfam	RF01385
Other data
RNA type	gene, sRNA
Domain(s)	Enterobacteriaceae
PDB structures	PDBe

An Hfq binding sRNA is an sRNA that binds the bacterial RNA binding protein called Hfq. A number of bacterial small RNAs which have been shown to bind to Hfq have been characterised (see list). Many of these RNAs share a similar structure composed of three stem-loops.^[1] Several studies have expanded this list, and experimentally validated a total of 64 Hfq binding sRNA in Salmonella Typhimurium.^[2]^[3]^[4] A transcriptome wide study on Hfq binding sites in Salmonella mapped 126 Hfq binding sites within sRNAs.^[5] Genomic SELEX has been used to show that Hfq binding RNAs are enriched in the sequence motif 5'-AAYAAYAA-3'.^[6]

Bacterial Hfq binding sRNAs

References

^ Zhang A, Wassarman KM, Rosenow C, Tjaden BC, Storz G, Gottesman S (2003). "Global analysis of small RNA and mRNA targets of Hfq". Mol. Microbiol. 50 (4): 1111–24. doi:10.1046/j.1365-2958.2003.03734.x. PMID 14622403.{{cite journal}}: CS1 maint: multiple names: authors list (link)
^ Pfeiffer V, Sittka A, Tomer R, Tedin K, Brinkmann V, Vogel J (December 2007). "A small non-coding RNA of the invasion gene island (SPI-1) represses outer membrane protein synthesis from the Salmonella core genome". Molecular Microbiology. 66 (5): 1174–91. doi:10.1111/j.1365-2958.2007.05991.x. PMID 17971080.{{cite journal}}: CS1 maint: multiple names: authors list (link)
^ Sittka A, Lucchini S, Papenfort K, et al. (2008). Burkholder WF (ed.). "Deep Sequencing Analysis of Small Noncoding RNA and mRNA Targets of the Global Post-Transcriptional Regulator, Hfq". PLOS Genetics. 4 (8): e1000163. doi:10.1371/journal.pgen.1000163. PMC 2515195. PMID 18725932.{{cite journal}}: CS1 maint: unflagged free DOI (link)
^ ^a ^b Padalon-Brauch G, Hershberg R, Elgrably-Weiss M, et al. (April 2008). "Small RNAs encoded within genetic islands of Salmonella typhimurium show host-induced expression and role in virulence". Nucleic Acids Research. 36 (6): 1913–27. doi:10.1093/nar/gkn050. PMC 2330248. PMID 18267966.
^ Holmqvist E, Wright PR, Li L, Bischler T, Barquist L, Reinhardt R, Backofen R, Vogel J (2016). "Global RNA recognition patterns of post-transcriptional regulators Hfq and CsrA revealed by UV crosslinking in vivo". EMBO J. doi:10.15252/embj.201593360. PMID 27044921.{{cite journal}}: CS1 maint: multiple names: authors list (link)
^ Lorenz C, Gesell T, Zimmermann B, Schoeberl U, Bilusic I, Rajkowitsch L, Waldsich C, von Haeseler A, Schroeder R (2010). "Genomic SELEX for Hfq-binding RNAs identifies genomic aptamers predominantly in antisense transcripts". Nucleic Acids Res. 38 (11): 3794–808. doi:10.1093/nar/gkq032. PMC 2887942. PMID 20348540.{{cite journal}}: CS1 maint: multiple names: authors list (link)