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Linker may refer to:
- Linker (computing), a computer program that takes one or more objects generated by a compiler and links them to standard library function and hence making them executable program
- Polylinker (also known as a multiple cloning site or simply a linker), a short segment of DNA with many restriction sites; commonly used in biotechnology, bioengineering, and molecular genetics
- Linker proteins (or adaptor proteins), proteins that provide mechanisms by which receptors can amplify and regulate downstream effector proteins, e.g.:
- Linker DNA, the part of a genomic DNA strand that connects two nucleosomes
- Amy Linker (born 1966), an American actress
- Irwin Linker (born 1939), an American creator of special-interest magazines
- Zita Linker (1917–2009), an Israeli politician
- Linker, a colloquial term for Inflation-indexed bonds (or linked bonds), the bonds for which the principal is indexed to inflation
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