MLST8

MLST8
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4JSN, 4JSP, 4JSV, 4JSX, 4JT5, 4JT6, 5FLC
Identifiers
Aliases	MLST8, GBL, GbetaL, LST8, POP3, WAT1, MTOR associated protein, LST8 homolog
External IDs	OMIM: 612190; MGI: 1929514; HomoloGene: 6833; GeneCards: MLST8; OMA:MLST8 - orthologs
Gene location (Human) Chr. Chromosome 16 (human) Band 16p13.3 Start 2,204,248 bp End 2,209,453 bp
Gene location (Mouse) Chr. Chromosome 17 (mouse) Band 17|17 A3.3 Start 24,692,525 bp End 24,698,052 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in right hemisphere of cerebellum right frontal lobe prefrontal cortex anterior cingulate cortex muscle of thigh apex of heart Brodmann area 9 amygdala nucleus accumbens left testis Top expressed in granulocyte neural layer of retina Ileal epithelium genital tubercle ventricular zone dentate gyrus of hippocampal formation granule cell spermatocyte primary visual cortex muscle of thigh cerebellar cortex More reference expression data BioGPS n/a
Gene ontology Molecular function protein binding protein serine/threonine kinase activator activity Cellular component cytoplasm nucleoplasm cytosol TORC1 complex TORC2 complex Biological process positive regulation of actin filament polymerization regulation of actin cytoskeleton organization regulation of GTPase activity positive regulation of peptidyl-tyrosine phosphorylation regulation of cellular response to heat TORC1 signaling positive regulation of protein serine/threonine kinase activity regulation of macroautophagy activation of protein kinase B activity TOR signaling positive regulation of TOR signaling Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 64223 56716 Ensembl ENSG00000167965 ENSMUSG00000024142 UniProt Q9BVC4 Q9DCJ1 RefSeq (mRNA) NM_001199173 NM_001199174 NM_001199175 NM_022372 NM_001352057 NM_001352059 NM_001352060 NM_001252463 NM_001252464 NM_001252465 NM_019988 RefSeq (protein) NP_001186102 NP_001186103 NP_001186104 NP_071767 NP_001338986 NP_001338988 NP_001338989 NP_001239392 NP_001239393 NP_001239394 NP_064372 Location (UCSC) Chr 16: 2.2 – 2.21 Mb Chr 17: 24.69 – 24.7 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Target of rapamycin complex subunit LST8, also known as mammalian lethal with SEC13 protein 8 (mLST8) or TORC subunit LST8 or G protein beta subunit-like (GβL or Gable), is a protein that in humans is encoded by the MLST8 (MTOR associated protein, LST8 homolog) gene. It is a subunit of both mTORC1 and mTORC2, complexes that regulate cell growth and survival in response to nutrient, energy, redox, and hormonal signals. It is upregulated in several human colon and prostate cancer cell lines and tissues. Knockdown of mLST8 prevented mTORC formation and inhibited tumor growth and invasiveness.

Further reading

• Ali SM, Sabatini DM (2005). "Structure of S6 kinase 1 determines whether raptor-mTOR or rictor-mTOR phosphorylates its hydrophobic motif site". J. Biol. Chem. 280 (20): 19445–8. doi:10.1074/jbc.C500125200. PMID 15809305.
• Rodgers BD, Levine MA, Bernier M, Montrose-Rafizadeh C (2001). "Insulin regulation of a novel WD-40 repeat protein in adipocytes". J. Endocrinol. 168 (2): 325–32. doi:10.1677/joe.0.1680325. PMID 11182770.
• Long X, Lin Y, Ortiz-Vega S, et al. (2005). "Rheb binds and regulates the mTOR kinase". Curr. Biol. 15 (8): 702–13. Bibcode:2005CBio...15..702L. doi:10.1016/j.cub.2005.02.053. PMID 15854902. S2CID 3078706.
• Kaizuka T, Hara T, Oshiro N, et al. (2010). "Tti1 and Tel2 are critical factors in mammalian target of rapamycin complex assembly". J. Biol. Chem. 285 (26): 20109–16. doi:10.1074/jbc.M110.121699. PMC 2888423. PMID 20427287.
• Loewith R, Jacinto E, Wullschleger S, et al. (2002). "Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control". Mol. Cell. 10 (3): 457–68. doi:10.1016/S1097-2765(02)00636-6. PMID 12408816.
• Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
• Sarbassov DD, Sabatini DM (2005). "Redox regulation of the nutrient-sensitive raptor-mTOR pathway and complex". J. Biol. Chem. 280 (47): 39505–9. doi:10.1074/jbc.M506096200. PMID 16183647.
• Oshiro N, Yoshino K, Hidayat S, et al. (2004). "Dissociation of raptor from mTOR is a mechanism of rapamycin-induced inhibition of mTOR function". Genes Cells. 9 (4): 359–66. doi:10.1111/j.1356-9597.2004.00727.x. hdl:20.500.14094/D1002969. PMID 15066126. S2CID 24814691.
• Inoki K, Ouyang H, Li Y, Guan KL (2005). "Signaling by target of rapamycin proteins in cell growth control". Microbiol. Mol. Biol. Rev. 69 (1): 79–100. doi:10.1128/MMBR.69.1.79-100.2005. PMC 1082789. PMID 15755954.
• Behrends C, Sowa ME, Gygi SP, Harper JW (2010). "Network organization of the human autophagy system". Nature. 466 (7302): 68–76. Bibcode:2010Natur.466...68B. doi:10.1038/nature09204. PMC 2901998. PMID 20562859.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Kawai S, Enzan H, Hayashi Y, et al. (2003). "Vinculin: a novel marker for quiescent and activated hepatic stellate cells in human and rat livers". Virchows Arch. 443 (1): 78–86. doi:10.1007/s00428-003-0804-4. PMID 12719976. S2CID 21552704.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2002). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Kim DH, Sarbassov DD, Ali SM, et al. (2003). "GbetaL, a positive regulator of the rapamycin-sensitive pathway required for the nutrient-sensitive interaction between raptor and mTOR". Mol. Cell. 11 (4): 895–904. doi:10.1016/S1097-2765(03)00114-X. PMID 12718876.
• Jacinto E, Loewith R, Schmidt A, et al. (2004). "Mammalian TOR complex 2 controls the actin cytoskeleton and is rapamycin insensitive". Nat. Cell Biol. 6 (11): 1122–8. doi:10.1038/ncb1183. PMID 15467718. S2CID 13831153.
• Sarbassov DD, Guertin DA, Ali SM, Sabatini DM (2005). "Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex". Science. 307 (5712): 1098–101. Bibcode:2005Sci...307.1098S. doi:10.1126/science.1106148. PMID 15718470. S2CID 45837814.