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This article is about the biochemical buffering agent. For information about other types of mops, see Mops (disambiguation).
IUPAC name
3-Morpholinopropane-1-sulfonic acid
Other names
3-(N-Morpholino)propanesulfonic acid,
3-Morpholinopropanesulfonic acid,
3-N-Morpholino propansulfonic acid,
4-Morpholinepropanesulfonic acid
3D model (Jmol)
ECHA InfoCard 100.013.162
Molar mass 209.26 g·mol−1
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references

MOPS (3-(N-morpholino)propanesulfonic acid) is a buffer introduced by Good et al. in the 1960s. It is a structural analog to MES.[1] Its chemical structure contains a morpholine ring. HEPES is a similar pH buffering compound that contains a piperazine ring. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.


MOPS is frequently used as a buffering agent in biology and biochemistry. It has been tested and recommended for polyacrylamide gel electrophoresis.[2] Usage above 20 mM in mammalian cell culture work is not recommended.[3]

See also[edit]


  1. ^ Good, Norman E.; Winget, G. Douglas; Winter, Wilhelmina; Connolly, Thomas N.; Izawa, Seikichi; Singh, Raizada M. M. (1966). "Hydrogen Ion Buffers for Biological Research". Biochemistry. 5 (2): 467–77. doi:10.1021/bi00866a011. PMID 5942950. 
  2. ^ Thomas, J; Hodes, ME (1981). "A new discontinuous buffer system for the electrophoresis of cationic proteins at near-neutral pH". Analytical Biochemistry. 118 (1): 194–6. doi:10.1016/0003-2697(81)90178-0. PMID 6278979. 
  3. ^ Eagle, H. (1971). "Buffer Combinations for Mammalian Cell Culture". Science. 174 (4008): 500–3. doi:10.1126/science.174.4008.500. PMID 5110427. 

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