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Protein NPLOC4 PDB 1nj3.png
Available structures
PDB Ortholog search: PDBe RCSB
Aliases NPLOC4, NPL4, NPL4 homolog, ubiquitin recognition factor
External IDs MGI: 2679787 HomoloGene: 5403 GeneCards: NPLOC4
Gene location (Human)
Chromosome 17 (human)
Chr. Chromosome 17 (human)[1]
Chromosome 17 (human)
Genomic location for NPLOC4
Genomic location for NPLOC4
Band 17q25.3 Start 81,556,887 bp[1]
End 81,648,465 bp[1]
RNA expression pattern
PBB GE NPLOC4 217796 s at fs.png
More reference expression data
Species Human Mouse
RefSeq (mRNA)



RefSeq (protein)



Location (UCSC) Chr 17: 81.56 – 81.65 Mb Chr 17: 120.38 – 120.44 Mb
PubMed search [3] [4]
View/Edit Human View/Edit Mouse

Nuclear protein localization protein 4 homolog is a protein that in humans is encoded by the NPLOC4 gene.[5][6][7]

Model organisms[edit]

Model organisms have been used in the study of NPLOC4 function. A conditional knockout mouse line, called Nploc4tm1a(EUCOMM)Wtsi[12][13] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists — at the Wellcome Trust Sanger Institute.[14][15][16]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[10][17] Twenty six tests were carried out and two phenotypes were reported. No homozygous mutant embryos were identified during gestation, and therefore none survived until weaning. The remaining tests were carried out on heterozygous mutant adult mice; no significant abnormalities were observed in these animals.[10]


NPLOC4 has been shown to interact with UFD1L.[5][18]


  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000182446 - Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000039703 - Ensembl, May 2017
  3. ^ "Human PubMed Reference:". 
  4. ^ "Mouse PubMed Reference:". 
  5. ^ a b Botta A, Tandoi C, Fini G, Calabrese G, Dallapiccola B, Novelli G (Sep 2001). "Cloning and characterization of the gene encoding human NPL4, a protein interacting with the ubiquitin fusion-degradation protein (UFD1L)". Gene. 275 (1): 39–46. doi:10.1016/S0378-1119(01)00649-7. PMID 11574150. 
  6. ^ Meyer HH, Shorter JG, Seemann J, Pappin D, Warren G (Jun 2000). "A complex of mammalian Ufd1 and Npl4 links the AAA-ATPase, p97, to ubiquitin and nuclear transport pathways". EMBO J. 19 (10): 2181–92. doi:10.1093/emboj/19.10.2181. PMC 384367Freely accessible. PMID 10811609. 
  7. ^ "Entrez Gene: NPLOC4 nuclear protein localization 4 homolog (S. cerevisiae)". 
  8. ^ "Salmonella infection data for Nploc4". Wellcome Trust Sanger Institute. 
  9. ^ "Citrobacter infection data for Nploc4". Wellcome Trust Sanger Institute. 
  10. ^ a b c Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88 (S248). doi:10.1111/j.1755-3768.2010.4142.x. 
  11. ^ Mouse Resources Portal, Wellcome Trust Sanger Institute.
  12. ^ "International Knockout Mouse Consortium". 
  13. ^ "Mouse Genome Informatics". 
  14. ^ Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410Freely accessible. PMID 21677750. 
  15. ^ Dolgin E (June 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718. 
  16. ^ Collins FS, Rossant J, Wurst W (January 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. 
  17. ^ van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biol. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837Freely accessible. PMID 21722353. 
  18. ^ Lass A, McConnell E, Fleck K, Palamarchuk A, Wójcik C (Aug 2008). "Analysis of Npl4 deletion mutants in mammalian cells unravels new Ufd1-interacting motifs and suggests a regulatory role of Npl4 in ERAD". Exp. Cell Res. 314 (14): 2715–23. doi:10.1016/j.yexcr.2008.06.008. PMID 18586029. 

Further reading[edit]

  • McConnell E, Lass A, Wójcik C (2007). "Ufd1-Npl4 is a negative regulator of cholera toxin retrotranslocation". Biochem. Biophys. Res. Commun. 355 (4): 1087–90. doi:10.1016/j.bbrc.2007.02.077. PMID 17331469. 
  • Gevaert K, Goethals M, Martens L, Van Damme J, Staes A, Thomas GR, Vandekerckhove J (2004). "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides". Nat. Biotechnol. 21 (5): 566–9. doi:10.1038/nbt810. PMID 12665801. 
  • Nagase T, Kikuno R, Ishikawa K, Hirosawa M, Ohara O (2000). "Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 7 (2): 143–50. doi:10.1093/dnares/7.2.143. PMID 10819331.