Naomi Ben-Ami was born in Chernivtsi, Ukraine, USSR, but because of her parents' work, she lived mostly in Kazakhstan. In September 1973 she and her family immigrated to Israel, just weeks before the outbreak of the Yom Kippur War. After spending time at an ulpan in Dimona, her parents took jobs as physicians in Jerusalem. Ben-Ami attended high school at Gymnasia Rehavia.
After graduating she entered the Israel Defense Forces, where she served in the Central Command as a secretary. Ben-Ami then studied international relations and Russian studies at Hebrew University of Jerusalem.
Ben-Ami is a married mother of two.
- Embassy of Israel in Kyiv
- Yossi Melman (26 October 2006). "Israeli ambassador to Ukraine appointed head of Nativ". Haaretz. Retrieved 13 January 2013.