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O3 may refer to:
- O3, the molecular formula for ozone
- O3, the 1993 debut album by the techno band Sunscreem
- O3: A Trilogy, a concept album trilogy by progressive metal band Dominici
- O3 star, in stellar classification, a subclass of type O stars
- O-3, the pay grade for the following officer ranks in the U.S. uniformed services:
- O3 Entertainment, a video game publisher
- Haplogroup O3 (Y-DNA), a Y-DNA Haplogroup
- Oldershaw O-3, glider
- USS O-3 (SS-64), a United States O class submarine
- Otoyol 3, a Turkish motorway from Edirne to Istanbul
- Oskarshamn 3, unit 3 at Oskarshamn Nuclear Power Plant in Sweden
| disambiguation page lists articles associated with the same title formed as a letter-number combination.
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