Required for meiotic nuclear division 5 homolog B (S. cerevisiae), also known as RMND5B, is a protein which in humans is encoded by the RMND5Bgene.[5] It has a zinc finger domain and is highly conserved throughout many eukaryotic organisms.
This depiction shows the location of the two predicted protein domains.Result of local sequence alignment- the amino acids found in the zinc finger domain are highlighted in different colorThe three amino acids which are coordinated to the zinc ion.
Two domains were predicted by the program BLIMPS[6] to exist in the protein of which one of the domains contains a zinc finger domain.
Zinc finger domains assist the binding of the protein to nucleic acids. This points to a direct interaction of CAD28476 with DNA during meiosis. By comparing CAD28476 with a related zinc finger protein[7] in a local sequence alignment using LALIGN,[8] the amino acids His359, Cys381 and Cys384 could be attributed to the zinc finger domain. This zinc finger structure is uncommon in the way that it involves one histidine instead of two.
Expression
Microarray data show that CAD28476 is highly expressed in tissue where meiosis occurs like in testis and ovaries. Moreover it is also highly expressed in the brain around the hypothalamus.
Transcriptional regulation
Listed binding sites for different transcription factors, the bold transcription factors are very likely to be involved in regulation.
gene encodes a putative RNA binding protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors.