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LOC101059915
Identifiers
Aliaseschromosome X open reading frame 49-like
External IDsHomoloGene: 131525; GeneCards: [1]; OMA:- orthologs
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_001323075

n/a

RefSeq (protein)

n/a

n/a

Location (UCSC)Chr X: 71.67 – 71.67 Mbn/a
PubMed search[2]n/a
Wikidata
View/Edit Human

LOC101059915 is a protein, which in humans is encoded by the LOC101059915 gene. It is located on the X chromosome and has restricted expression in the testis.

Gene

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The LOC101059915 gene has two aliases known as chromosome X open reading frame 49-like and BX276092.6.[3]

Locus and Structure

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LOC101059915 is located on the X chromosome at locus Xq13.1. It is 1,831 base pairs long and the gene sequence has 6 exons.[4] LOC101059915 also has one protein coding transcript.

Promoter Region and Expression

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The promoter region of LOC101059915 is located on the sense strand of DNA, and between base pair 71666098 and 71667904 on the X chromosome. It spans up to 1.806 bp.[5] Expression of LOC101059915, however, is relatively low in human cells, and is primarily limited to the testis.[6]

Protein

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General Features and Compositional Analysis

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The protein has 518 amino acids and a molecular mass of 55.1 kDa.[7] The isoelectric point is 8.15. Compared to other human proteins LOC101059915 is glycine-, proline-rich, and serine-rich but the protein has lower levels of tyrosine.[8]

Domains

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The domain of unknown function, DUF4641 covers almost the entire protein. It is a part of pfam15483.[9] It is 410 amino acids long, from amino acid 85 until amino acid number 495.[10]

Schematic illustration made using DOG software showing the domain of unknown function (DUF4641) as well as the location of secondary structures such as alpha helices, and post-translation modifications such as SUMO sites.

Secondary Structure

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The secondary structure of LOC101059915 has been shown to consist of primarily alpha helices as determined by models made on I-TASSER and analysis using ExPASy tools.[11]

Displays the modeled secondary structures of LOC101059915 with the red indicating alpha helices, and the yellow indicating possible beta sheets.

Post-translation modifications

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LOC101055915 is predicted to contain many different post-translational modifications. This include sites for phosphorylation (NetPhos 2.0[12]) and sumoylation (SUMOplot Analysis Program[13]).

Subcellular localization

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The LOC101059915 protein has been predicted to be located in the cell nucleus (PSORT II).[14]

Homology and Evolution

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Paralogs

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CXorf49 and CXorf49B are paralogs of LOC101059915. They share upwards of 78% similarity with LOC101059915 and likely went through a gene duplication event relatively recently, in evolutionary terms, due the high degree of conservation between all three sequences.

CXorf49 is especially interesting due it being shown to be involved as one of the components of a small group of the HL-60 cell proteome that are most prone to form 4-Hydroxy-2-nonenal(HNE) adducts, upon exposure to nontoxic (10 μM) HNE concentrations, along with heat shock 60 kDa protein 1.[15]

Orthologs

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Using BLAST[16] no orthologs for LOC101059915 are found in single celled organisms, fungi or plants whose genomes have been sequenced. For multi-cellular organisms, orthologs are found in mammals, excluding Monotremes. The table below shows a representative sample of 20 of the orthologs for LOC101059915. The table is organized based on the time of divergence from humans in millions of years (MYA). In cases where the divergence time-frame is the same the orthologs are sorted by identity (%).

Genus and Species Name Common name Divergence from Human Lineage (MYA) Accession Number Sequence length (aa) Sequence Identity to Human Protein Sequence Similarity to Human Protein
Pan paniscus Chimpanzee 6.65 XP_003820175.1 518 97% 97%
Papio

anubis

Olive Baboon 29.4 XP_003919408.1 514 70 % 77%
Piliocolobus tephrosceles Ugandan Red

Colobus

29.4 XP_023050488.1 514 68 % 76%
Cebus capucinus imitator White-Headed

Capuchin

43 XP_017372264.1 472 68 % 72%
Callithrix jacchus Common

Marmoset

43 XP_008987720.1 474 67% 72%
Aotus nancymaae Nancy Ma’s

Night Monkey

43 XP_010822786.1 432 63% 66%
Saimiri boliviensis

boliviensis

Black-capped

Squirrel Monkey

43 XP_003944512.1 521 58% 68%
Rattus norvegicus Brown Rat 90 XP_008756683.2 136 46% 55%
Ictidomys

tridecemlineatus

Thirteen-Lined

Ground Squirrel

90 XP_021576972.1 565 45% 57%
Canis lupus familiaris Dog 96 XP_850392.2 526 52% 64%
Panthera pardus Leopard 96 XP_019284263.1 472 47% 58%
Enhydra lutris kenyoni Sea Otter 96 XP_022348355.1 495 47% 59%
Ovis aries musimon Mouflon 96 XP_01208848.1 539 45% 56%
Capra hircus Goat 96 XP_017899210.1 538 44% 55%
Pantholops hodgsonii Tibetan Antelope 96 XP_005965061.1 533 44% 55%
Myotis lucifugus Little brown bat 96 XP_006083036.1 500 42 % 55%
Trichechus manatus latirostris Florida manatee 105 XP_012415455.1 505 44% 55%
Orycteropus afer afer Aardvark 105 XP_007957133.1 477 39% 50%
Phascolarctos cinereus Koala 159 XP_020834608.1 474 37% 50%
Sarcophilus harrisii Tasmanian Devil 159 XP_023362890.1 576 33% 50%

Phylogeny

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Shows the unrooted branching of select orthologs for LOC10105519.

The most distant ortholog for LOC101059915 is from the species Sarcrophilus harrisii which is commonly known as the Tasmanian Devil dating from more than 159.0 million years ago.

References

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  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000283599Ensembl, May 2017
  2. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  3. ^ "LOC101055915". GeneCards. Retrieved 2018-02-05.
  4. ^ "LOC101055915 [Homo sapiens (human)] - Gene - NCBI". Ncbi.nlm.nih.gov. Retrieved 2018-04-28.
  5. ^ Genomatix’s ElDorado
  6. ^ "EST Profile - Hs.632817". Ncbi.nlm.nih.gov. Retrieved 2018-04-28.
  7. ^ "LOC101059915". ExPASy pI/mW tool. Retrieved 2018-04-28.
  8. ^ "SDSC Biology Workbench". seqtool.sdsc.edu. Retrieved 2018-05-06.
  9. ^ "NCBI CDD Conserved Protein Domain DUF4641". www.ncbi.nlm.nih.gov. Retrieved 2018-05-06.
  10. ^ "uncharacterized protein LOC101059915 [Homo sapiens] - Protein - NCBI". Ncbi.nlm.nih.gov. Retrieved 2018-01-28.
  11. ^ "LOC101059915 Secondary Structure Model". Zhang Lab I-TASSER. Retrieved 2018-02-05.
  12. ^ "NetPhos 2.0 Server". Cbs.dtu.dk. Retrieved 2018-04-28.
  13. ^ "SUMOplot Analysis Program". Abgent. Retrieved 2018-04-28.
  14. ^ "PSORT II Server". Retrieved 2018-05-04.
  15. ^ Arcaro, Alessia; Daga, Martina; Cetrangolo, Giovanni Paolo; Ciamporcero, Eric Stefano; Lepore, Alessio; Pizzimenti, Stefania; Petrella, Claudia; Graf, Maria; Uchida, Koji; Mamone, Gianfranco; Ferranti, Pasquale; Ames, Paul R. J.; Palumbo, Giuseppe; Barrera, Giuseppina; Gentile, Fabrizio (2015). "Generation of Adducts of 4-Hydroxy-2-nonenal with Heat Shock 60 kDa Protein 1 in Human Promyelocytic HL-60 and Monocytic THP-1 Cell Lines". Oxidative Medicine and Cellular Longevity. 2015: 296146. doi:10.1155/2015/296146. PMC 4452872. PMID 26078803.
  16. ^ Protein BLAST