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Colony hybridization is the process analyzing a certain genetic sequence of DNA, RNA, Proteins, Antibodies, Enzymes,[1] or bacteria colonies.[2] Hybridization has high specificity which allows it to find a specific sequence, even when it is surrounded by similar sequences.[1]


  1. A cell culture is grown until it has a minimum of 50 colonies.[3]
  2. A sample of the culture is transferred onto nylon[3] or nitrocellulose[1] sheet.


  1. ^ a b c Melanie Overstreet. "Colony Hybridization". Retrieved 2010-02-26. 
  2. ^ Maier, Raina (2009). Environmental Microbiology. Academic Press. p. 248. ISBN 9780123705198. 
  3. ^ a b "Colony Hybridization Protocol" (PDF). 1999. Retrieved 2010-02-26.