ZNF184

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ZNF184
Identifiers
Aliases ZNF184, kr-ZNF3, zinc finger protein 184
External IDs MGI: 1922244 HomoloGene: 113602 GeneCards: ZNF184
Orthologs
Species Human Mouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_007149
NM_001318891
NM_001318892
NM_001318893
NM_001347832

NM_183014

RefSeq (protein)

NP_001305820
NP_001305821
NP_001305822
NP_001334761
NP_009080

NP_898835.1
NP_898835

Location (UCSC) Chr 6: 27.45 – 27.47 Mb Chr 13: 21.95 – 21.96 Mb
PubMed search [1] [2]
Wikidata
View/Edit Human View/Edit Mouse

Zinc finger protein 184, also known as ZNF184, is a protein that in humans is encoded by the ZNF184 gene[3] on chromosome 6. It was first identified by Goldwurm et al. in 1996.[4]

The National Center for Biotechnology Information (NCBI) Gene database entry[3] for ZNF184 identifies conserved domains KRAB_A (Krüppel associated box) near the N-terminus and Zn-finger (Zinc finger) at the C-terminus of the translated protein. The former is associated with transcription repression[5] and the latter with DNA binding (see Zinc finger).

Domains and Structure[edit]

The figure below is a reformatted and annotated conceptual translation display of ZNF184's Consensus CDS.[6] CCDS displays exons in alternating black and blue font, with red indicating a residue coded across a splice boundary.

ZNF184 has 19 zinc finger motifs at the end of its final and longest exon. The figure shows regularity among the fingers in this protein, including the 2 columns of green-highlighted Cysteine residues and the 2 columns of blue-highlighted His residues which are the reason this type of zinc finger is called C2H2. Light grey highlighted columns (one with all F; one with mostly L, and F substitutions) are highly conserved hydrophobic residues within the zinc finger motif. The other light grey highlighted column (mostly K, with a similar R substitution) is an example of fairly strong conservation in the coil sections connecting adjacent fingers.

Near the N-terminus is a KRAB_A domain followed by a KRAB_B domain. KRAB_A has a shorter α-Helix followed by a longer α-Helix. The KRAB_A motif in a zinc finger protein is known to bind with a KAP-1 protein (aka TRIM28) to accomplish a transcription repressor function, however a gene so regulated by ZNF184 has yet to be identified. The length-11 finger helices are indicated, as well as the overlapping 7-residue section in each finger which binds targeted DNA (if the finger is functioning).

Reformatted Protein

References[edit]

  1. ^ "Human PubMed Reference:". 
  2. ^ "Mouse PubMed Reference:". 
  3. ^ a b "Entrez Gene: zinc finger protein 184". 
  4. ^ Goldwurm S, Menzies ML, Banyer JL, Powell LW, Jazwinska EC (1997-03-15). "Identification of a novel Krueppel-related zinc finger gene (ZNF184) mapping to 6p21.3.". Genomics. 40 (3): 486–9. doi:10.1006/geno.1996.4583. PMID 9073517. 
  5. ^ Peng H, Begg GE, Harper SL, Friedman JR, Speicher DW, Rauscher FJ 3rd (2000-03-30). "Biochemical Analysis of the Kruppel-associated Box (KRAB) Transcriptional Repression Domain". Journal of Biological Chemistry. 275 (24): 18000–10. doi:10.1074/jbc.M001499200. PMID 10748030. 
  6. ^ "Consensus CDS: zinc finger protein 184". 

Further reading[edit]