Intestinal epithelium: Difference between revisions
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Although they primarily function as part of the [[digestive system]], [[enterocyte]]s of the intestinal epithelium also express [[toll-like receptor]]s and [[NOD-like receptor|nucleotide oligomerization domain]] proteins that recognize diverse types of microbes and contribute to [[immune system]] function.<ref>{{Cite journal|title = TLR signaling in the gut in health and disease|url = https://www.ncbi.nlm.nih.gov/pubmed/15814663?dopt=Abstract|journal = Journal of Immunology (Baltimore, Md.: 1950)|date = 2005-04-15|issn = 0022-1767|pmid = 15814663|pages = 4453–4460|volume = 174|issue = 8|first = Maria T.|last = Abreu|first2 = Masayuki|last2 = Fukata|first3 = Moshe|last3 = Arditi}}</ref> Thus the intestinal epithelium not only serves as a physical barrier separating the intestinal lumen from the body proper but also carries out pathogen recognition functions as part of the [[Intrinsic immunity|intrinsic immune system]]. |
Although they primarily function as part of the [[digestive system]], [[enterocyte]]s of the intestinal epithelium also express [[toll-like receptor]]s and [[NOD-like receptor|nucleotide oligomerization domain]] proteins that recognize diverse types of microbes and contribute to [[immune system]] function.<ref>{{Cite journal|title = TLR signaling in the gut in health and disease|url = https://www.ncbi.nlm.nih.gov/pubmed/15814663?dopt=Abstract|journal = Journal of Immunology (Baltimore, Md.: 1950)|date = 2005-04-15|issn = 0022-1767|pmid = 15814663|pages = 4453–4460|volume = 174|issue = 8|first = Maria T.|last = Abreu|first2 = Masayuki|last2 = Fukata|first3 = Moshe|last3 = Arditi}}</ref> Thus the intestinal epithelium not only serves as a physical barrier separating the intestinal lumen from the body proper but also carries out pathogen recognition functions as part of the [[Intrinsic immunity|intrinsic immune system]]. |
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===Importance for human health=== |
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Loss of integrity of the intestinal epitelium plays a key pathogenic role in intestinal bowel disease (IBD).<ref>{{Cite journal|last=Maloy|first=Kevin J.|last2=Powrie|first2=Fiona|date=2011-06-16|title=Intestinal homeostasis and its breakdown in inflammatory bowel disease|url=https://www.ncbi.nlm.nih.gov/pubmed/21677746|journal=Nature|volume=474|issue=7351|pages=298–306|doi=10.1038/nature10208|issn=1476-4687|pmid=21677746}}</ref> Changes in the composition of the intestinal microbiota are an important environmental factor in the development of IBD. Detrimental changes in the intestinal microbiota induce an inappropriate (uncontrolled) immune response that results in damage to the intestinal epithelium. Breaches in this critical barrier (the intestinal epithelium) allow further infiltration of microbiota that, in turn, elicits further immune responses. IBD is a multifactorial disease that is nonetheless driven in part by an exaggerated immune response to gut microbiota that cause defects in epithelial barrier function.<ref>{{Cite journal|last=Coskun|first=Mehmet|date=2014-08-25|title=Intestinal Epithelium in Inflammatory Bowel Disease|url=http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4292184/|journal=Frontiers in Medicine|volume=1|doi=10.3389/fmed.2014.00024|issn=2296-858X|pmc=4292184|pmid=25593900}}</ref> |
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==References== |
==References== |
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Revision as of 18:29, 10 April 2016
| Intestinal epithelium | |
|---|---|
 Simple columnar epithelial cells | |
| Identifiers | |
| MeSH | D007413 |
| FMA | 15695 17229, 15695 |
| Anatomical terminology | |
| This article is part of a series on |
| Epithelia |
|---|
| Squamous epithelial cell |
| Columnar epithelial cell |
| Cuboidal epithelial cell |
| Specialised epithelia |
|
| Other |
 |
| This article is one of a series on the |
| Gastrointestinal wall |
|---|
The intestinal epithelium is the layer of cells that forms the luminal surface of both the small and large intestine (colon) of the gastrointestinal tract. It is composed of noncilliated simple columnar cells. It has two important functions: absorbing helpful substances and providing a barrier against harmful substances. Some diseases and conditions are caused by dysfunction in the intestinal epithelium, and some diseases and conditions cause problems with these cells, which then leads to further complications.
Structure
The epithelium is part of the intestinal mucosa layer. The epithelium is composed of a single layer of cells. The other two layers of the mucosa, the lamina propria and the muscularis mucosae, support and articulate the epithelium layer. To securely contain of the contents of the intestinal lumen, the cells of the epithelial layer are are joined together by tight junctions thus forming a contiguous and relatively impermeable membrane.
Epithelial cells are continuously renewed every 4–5 days through a process of renewal and migration. Renewal relies on proliferative cells (stem cells) that reside at the base of the crypts of Lieberkühn (epithelial invasions into the underlying connective tissue).[1] After being formed at the base, the new cells migrate upwards and out of the crypt. Eventually, they undergo apoptosis and are shed off into the intestinal lumen.[2] In this way, the total number of cells making up the intestinal epithelium remains constant.[3]
In the small intestine, the mucosal layer is specially adapted to provide a large surface area in order to maximize the absorption of nutrients. The expansion of the absorptive surface, 600 times beyond that of a cylindrical tube, is achieved by three anatomical factors:[4]
- Circular folds (valves of Kerckring) are transverse folds that slow the passage of the luminal contents and serve to expand the total surface area three fold.
- Villi and intestinal glands (also crypts of Lieberkühn) serve to increase the mucosal surface area ten fold. (Intestinal villus)
- Microvilli covering the apical surface of the enterocytes increase the absorptive surface twenty fold. These numerous microscopic (100 nanometers in diameter) finger-like projections form an undulated brush border.
The brush border on the apical surface of the epithelial cells is covered with glycocalyx, which is composed of oligosaccharides attached to membrane glycoproteins and glycolipids.[5]
Cell types
Six different cell types are produced by the stem cells that reside at the base of the crypts.[6] Each type matures according to its specific differentiation program as it migrates up and out of the crypt. Many of genes necessary for differentiation into the different epithelial cell types have been identified and characterized (see this table). The cell types produced are: enterocytes, Goblet cells, enteroendocrine cells, Paneth cells, microfold cells, cup cells and tuft cells. Their functions are listed here:[7]
- Enterocytes are the most numerous and function primarily for nutrient absorption. Enterocyes express many catablolic enzymes on their exterior luminal surface to break down molecules to sizes appropriate for uptake into the cell. Examples of molecules taken up by enterocytes are: ions, water, simple sugars, vitamins, lipids, peptides and amino acids.
- Goblet cells secrete the mucus layer that protects the epitheilium from the lumenal contents.
- Enteroendocrine cells secrete various gastrointestinal hormones including secretin, pancreozymin, enteroglucagon among others.
- Paneth cells produce antimicrobial peptides such as human beta-defensin.[8][9]
- Microfold cells (commonly referred to as M cells) sample antigens from the lumen and deliver them to the lymphoid tissue associated with the mucosa (MALT). In the small intestine, M cells are associated with Peyer’s patches.
- Cup cells are a distinct cell type but with no known function.
- Tuft cells play a part in the immune response.[10]
Throughout the digestive tract, the distribution of the different types of epithelial cells varies according the function of that region.[3]
Structural components of cellular junctions
Important for the barrier function of intenstinal epithelium, its cells are joined securely together by four types of junctions (cell junctions),[11] which can be identified at the ultrastructural level:[12]
Gap junctions
Gap junctions bring the adjacent cells within 2 nanometers of each other. They are formed by several homologous proteins encoded by the connexin gene family coming together to form a multiprotein complex. The molecular structure of this complex is in the form of a hexamer. The complex, which is embedded in the cell walls of the two joined cells, forms a gap or channel in the middle of the six proteins. This channel allows various molecules, ions and electrical impulses to pass between the two cells.[13]
Desmosomes
These complexes, consisting of transmembrane adhesion proteins of the cadherin family, link adjacent cells together through their cytoskeletons.[14] Desmosomes leave a gap of 30 nanometers between cells.[13]
Adherens junctions
Adherens junctions, also called zonula adherens, are multiprotein complexes formed by proteins of the catenin and cadherin families. They are located in the membrane at the contact points between the cells. They are formed by interactions between intracellular adapter proteins, transmembrane proteins and the actin cytoskeletons of the cells . Besides their role in linking adjacent cells, these complexes are important for regulating epithelial migration, cell polarity, and the formation of other cell junction complexes.[12]
Tight junctions
Tight junctions, also called zonula occludens, are the most important components of the intenstinal epithelium for its barrier function.[15] These complexes, formed primarily of members of the claudin and the occludin families, consist of about 35 different proteins,[11] form a ring shaped continuous ribbon around the cells, and are located near the borders of the lateral and apical membranes.[12]
The extracellular domains of the transmembrane proteins in adjacent cells cross connect to form a tight seal. These interactions include those between proteins in the same membrane ("cis") and proteins in adjacent cells ("trans"). In addition, interactions can be homophilic (between identical proteins) or heterophilic (between different proteins).[12]
Similar to adherens junctions, the intracellular domains of tight junctions interact with different scaffold proteins, adapter proteins and signaling complexes to regulate cytoskeletal linking, cell polarity, cell signaling and vesical trafficing.[12]
Tight junctions provide a narrow but modifiable seal between adjacent cells in the epithelial layer and thereby provide selective paracellular transport of solutes.[12] While previously thought to be static structures, tight junctions are now known to be dynamic and can change the size of the opening between cells and thereby adapt to the different states of development, physiologies and pathologies.[15] They function as a selective and semipermeable paracellular barrier between apical and basolateral compartments of the epithelial layer. They function to facilitate the passage of small ions and water-soluble solutes through the paracellular space while preventing the passage of of luminal antigens, microorganisms and their toxins.[12]
Physiology
The intestinal epithelium has a complex anatomical structure which facilitates coordinated digestive, absorptive, motility, immunological and neuroendocrine functions.[16]
The mucus secreted by goblet cells acts as a lubricant and protects the epithelial cell layer against irritation from mucosal contents.[17]
Traditionally, crypt cells were considered primarily as secretory cells while enterocytes are considered principally absorptive. However, recent studies have challenged this classical functional partitioning and have shown that both the surface and crypt cells can perform both secretory and absorptive functions and that, in fact, these functions can occur simultaneously.[18][19]
Nutrient uptake
Overlaying the brush border of the apical surface of the enterocytes is the glycocalyx, which is a loose network composed of the oligosaccharide side chains of integral membrane hydrolases and other enzymes essential for the digestion of proteins and carbohydrates. These glycoproteins, glycolipids, and enzymes catalyze the final digestive stages of luminal carbohydrates and proteins. The monosaccharides and amino acids thus produced are subsequently transported across the intestinal epithelium and eventually into the bloodstream.[5]
The absorption of electrolytes and water is one of the most important functions of the digestive tract. Water absorption is passive and isotonic - depending on the speed and direction of solute flow. Other factors influencing fluid absorption are osmolarity and the specific intestinal region.[16] Regulated selective permability is performed through two major routes: the transcellular (transepithelial) route and the paracellular route.[12]
Transcellular permeability
This consists of specific transport of solutes across the epithelial cells. It is predominantly regulated by the activities of speciallized transporters that translocate specific electrolytes, amino acids, sugars, short chain fatty acids and other molecules into or out of the cell.[12]
Paracellular permeability
Paracellular permeability depends on transport through the spaces that exist between epithelial cells. It is regulated by cellular junctions that are localized in the laminal membranes of the cells.[12] This is the main route of passive flow of water and solutes across the intestinal epithelium. Regulation depends on the intercellular tight junctions which have the most influence on paracellular transport.[20] Studies using the electron microscope showed that the electrical resistance of epithelial layers depends on the complexity and number of filaments within the tight junction transmembrane protein complexes.[16] Also, the plasma membrane resistance and variable transmembrane conductance of the epithelial cells can also modulate paracellular pathway function.[16]
Functions
The barrier formed by the intestinal epithelium separates the external environment (the contents of the intestinal lumen) from the body[12] and is the most extensive and important mucosal surface of body.[15]
The intestinal epithelium serves two crucial functions. First, it acts as a barrier, preventing the entry of harmful substances such as foreign antigens, toxins and microorganisms.[11][12] Second, it acts as a selective filter which facilitates the uptake of dietary nutrients, electrolytes, water and various other beneficial substances from the intestinal lumen.[12]
When barrier integrity is lost, intestinal permeability increases and uncontrolled passage of harmful substances can occur. This can lead to, depending on the genetic predisposition of the individual, the development of inflammation, infection, allergies, autoimmune diseases or cancer - within the intestine itself or other organs.[16]
Although they primarily function as part of the digestive system, enterocytes of the intestinal epithelium also express toll-like receptors and nucleotide oligomerization domain proteins that recognize diverse types of microbes and contribute to immune system function.[21] Thus the intestinal epithelium not only serves as a physical barrier separating the intestinal lumen from the body proper but also carries out pathogen recognition functions as part of the intrinsic immune system.
Importance for human health
Loss of integrity of the intestinal epitelium plays a key pathogenic role in intestinal bowel disease (IBD).[22] Changes in the composition of the intestinal microbiota are an important environmental factor in the development of IBD. Detrimental changes in the intestinal microbiota induce an inappropriate (uncontrolled) immune response that results in damage to the intestinal epithelium. Breaches in this critical barrier (the intestinal epithelium) allow further infiltration of microbiota that, in turn, elicits further immune responses. IBD is a multifactorial disease that is nonetheless driven in part by an exaggerated immune response to gut microbiota that cause defects in epithelial barrier function.[23]
References
- ^ Clevers H (2013). "The intestinal crypt, a prototype stem cell compartment". Cell. 154 (2): 274–84. doi:10.1016/j.cell.2013.07.004. PMID 23870119.
- ^ van der Flier, Laurens G.; Clevers, Hans (1 January 2009). "Stem cells, self-renewal, and differentiation in the intestinal epithelium". Annual Review of Physiology. 71: 241–260. doi:10.1146/annurev.physiol.010908.163145. ISSN 1545-1585. PMID 18808327.
- ^ a b Lodish, Harvey; Berk, Arnold; Zipursky, S. Lawrence; Matsudaira, Paul; Baltimore, David; Darnell, James (1 January 2000). "Intestinal Architecture and Development". Molecular Cell Biology (4th ed.). W. H. Freeman. ISBN 0716731363.
- ^ Khurana (1 January 2005). Textbook Of Medical Physiology. Elsevier India. p. 641. ISBN 9788181478504.
- ^ a b Lodish, Harvey; Berk, Arnold; Zipursky, S. Lawrence; Matsudaira, Paul; Baltimore, David; Darnell, James (1 January 2000). "Transport across Epithelia".
{{cite journal}}: Cite journal requires|journal=(help) - ^ Laurens G. van der Flier; Hans Clevers (2009). "Stem Cells, Self-Renewal, and Differentiation in the Intestinal Epithelium". Annual Review of Physiology. 71 (1): 241–260. doi:10.1146/annurev.physiol.010908.163145. PMID 18808327. Retrieved 14 February 2014.
{{cite journal}}: Unknown parameter|deadurl=ignored (|url-status=suggested) (help) - ^ Sarmento, Bruno (30 September 2015). Concepts and Models for Drug Permeability Studies: Cell and Tissue based In Vitro Culture Models. Woodhead Publishing. pp. 57–58. ISBN 9780081001141.
- ^ van Es, Johan H.; Clevers, Hans (16 June 2014). "Paneth cells". Current biology: CB. 24 (12): R547–548. doi:10.1016/j.cub.2014.04.049. ISSN 1879-0445. PMID 24937274.
- ^ Santaolalla R, Abreu MT (2012). "Innate immunity in the small intestine". Curr Opin Gastroenterol. 28 (2): 124–9. doi:10.1097/MOG.0b013e3283506559. PMC 3502878. PMID 22241076.
- ^ Gerbe, F; Legraverend, C; Jay, P (September 2012). "The intestinal epithelium tuft cells: specification and function". Cellular and molecular life sciences : CMLS. 69 (17): 2907–17. PMC 3417095. PMID 22527717.
- ^ a b c Khan, Niamat; Asif, Abdul R. (1 January 2015). "Transcriptional Regulators of Claudins in Epithelial Tight Junctions". Mediators of Inflammation. 2015. doi:10.1155/2015/219843. ISSN 0962-9351. PMC 4407569. PMID 25948882.
{{cite journal}}: CS1 maint: unflagged free DOI (link) - ^ a b c d e f g h i j k l m Groschwitz, Katherine R.; Hogan, Simon P. (1 July 2009). "Intestinal Barrier Function: Molecular Regulation and Disease Pathogenesis". The Journal of allergy and clinical immunology. 124 (1): 3–22. doi:10.1016/j.jaci.2009.05.038. ISSN 0091-6749. PMC 4266989. PMID 19560575.
- ^ a b Bennett, M. V.; Barrio, L. C.; Bargiello, T. A.; Spray, D. C.; Hertzberg, E.; Sáez, J. C. (1 March 1991). "Gap junctions: new tools, new answers, new questions". Neuron. 6 (3): 305–320. ISSN 0896-6273. PMID 1848077.
- ^ Nekrasova, Oxana; Green, Kathleen J. (1 November 2013). "Desmosome assembly and dynamics". Trends in cell biology. 23 (11): 537–546. doi:10.1016/j.tcb.2013.06.004. ISSN 0962-8924. PMC 3913269. PMID 23891292.
- ^ a b c Rao, Jaladanki N.; Wang, Jian-Ying (1 January 2010). "Intestinal Architecture and Development".
{{cite journal}}: Cite journal requires|journal=(help) - ^ a b c d e Fasano, Alessio (1 January 2011). "Zonulin and Its Regulation of Intestinal Barrier Function: The Biological Door to Inflammation, Autoimmunity, and Cancer". Physiological Reviews. 91 (1): 151–175. doi:10.1152/physrev.00003.2008. ISSN 0031-9333. PMID 21248165.
- ^ Allen, Adrian; Flemström, Gunnar (1 January 2005). "Gastroduodenal mucus bicarbonate barrier: protection against acid and pepsin". American Journal of Physiology. Cell Physiology. 288 (1): C1–19. doi:10.1152/ajpcell.00102.2004. ISSN 0363-6143. PMID 15591243.
- ^ Geibel, John P. (1 January 2005). "Secretion and absorption by colonic crypts". Annual Review of Physiology. 67: 471–490. doi:10.1146/annurev.physiol.67.031103.153530. ISSN 0066-4278. PMID 15709966.
- ^ Binder, Henry J.; Rajendran, Vazhaikkurichi; Sadasivan, Vidyasagar; Geibel, John P. (1 April 2005). "Bicarbonate secretion: a neglected aspect of colonic ion transport". Journal of Clinical Gastroenterology. 39 (4 Suppl 2): S53–58. ISSN 0192-0790. PMID 15758660.
- ^ Näslund, Erik; Hellström, Per M. (10 September 2007). "Appetite signaling: from gut peptides and enteric nerves to brain". Physiology & Behavior. 92 (1–2): 256–262. doi:10.1016/j.physbeh.2007.05.017. ISSN 0031-9384. PMID 17582445.
- ^ Abreu, Maria T.; Fukata, Masayuki; Arditi, Moshe (15 April 2005). "TLR signaling in the gut in health and disease". Journal of Immunology (Baltimore, Md.: 1950). 174 (8): 4453–4460. ISSN 0022-1767. PMID 15814663.
- ^ Maloy, Kevin J.; Powrie, Fiona (16 June 2011). "Intestinal homeostasis and its breakdown in inflammatory bowel disease". Nature. 474 (7351): 298–306. doi:10.1038/nature10208. ISSN 1476-4687. PMID 21677746.
- ^ Coskun, Mehmet (25 August 2014). "Intestinal Epithelium in Inflammatory Bowel Disease". Frontiers in Medicine. 1. doi:10.3389/fmed.2014.00024. ISSN 2296-858X. PMC 4292184. PMID 25593900.
{{cite journal}}: CS1 maint: unflagged free DOI (link)