The cytoskeleton is a network of fibers composed of proteins contained within a cell's cytoplasm. Although the name implies the cytoskeleton to be stable, it is a dynamic structure, parts of which are constantly destroyed, renewed or newly constructed.
In all cells of all domains of life (archaea, bacteria, eukaryotes) a cytoskeleton is found (notably in all eukaryotic cells which includes human, animal and plant cells). The cytoskeletal systems of different organisms are composed by similar proteins. However, structure, function and dynamic behaviour of the cytoskeleton can be very different, depending on organism and cell type. Similarly, within the same cell type the structure, dynamic behaviour, and function of the cytoskeleton can change through association with other proteins and the previous history of the network.
The cytoskeleton of eukaryotes (including human and all animals cells) has three major components: microfilaments composed of the protein actin and microtubules composed of the protein tubulin are present in all prokaryotic cells. By contrast intermediate filaments, which have more that 60 different building block proteins have so far only been found in animal cells (apart from one non-eukaryotic bacterial intermediate filament crescentin). The complexity of the eukaryotic cytoskeleton emerges from the interaction with hundreds of associated proteins like molecular motors, crosslinkers, capping proteins and nucleation promoting factors.
There is a multitude of functions the cytoskeleton can perform: It gives the cell shape and mechanical resistance to deformation; through association with extracellular connective tissue and other cells it stabilizes entire tissues; it can actively contract, thereby deforming the cell and the cell's environment and allowing cells to migrate; it is involved in many cell signaling pathways; it is involved in the uptake of extracellular material (endocytosis); it segregates chromosomes during cellular division; it is involved in cytokinesis - the division of a mother cell into two daughter cells; it provides a scaffold to organize the contents of the cell in space  and for intracellular transport (for example, the movement of vesicles and organelles within the cell); it can be a template for the construction of a cell wall. Furthermore, it forms specialized structures such as flagella, cilia, lamellipodia and podosomes.
A large scale example of an action performed by the cytoskeleton is muscle contraction. During contraction of a muscle, within each muscle cell, myosin molecular motors collectively excert forces on parallel actin filaments. This action contracts the muscle cell, and through the synchronous process in many muscle cells, the entire muscle.
- 1 Eukaryotic cytoskeleton
- 2 Prokaryotic cytoskeleton
- 3 Common features and differences between prokaryotes and eukaryotes
- 4 History
- 5 See also
- 6 References
- 7 External links
Eukaryotic cells contain three main kinds of cytoskeletal filaments: microfilaments which are composed of actin, intermediate filaments which have around 70 different proteins as building blocks, and microtubules with tubulin as the basic subunit. The cytoskeleton provides the cell with structure and shape, and by excluding macromolecules from some of the cytosol, it adds to the level of macromolecular crowding in this compartment. Cytoskeletal elements interact extensively and intimately with cellular membranes. A number of small molecule cytoskeletal drugs have been discovered that interact with actin and microtubules. These compounds have proven useful in studying the cytoskeleton and several have clinical applications.
Microfilaments (actin filaments)
Microfilaments are one of the thinnest filaments of the cytoskeleton. They are composed of linear polymers of G-actin subunits, and generate force when the growing (plus) end of the filament pushes against a barrier, such as the cell membrane. They also act as tracks for the movement of myosin molecules that attach to the microfilament and "walk" along them. Myosin motoring along F-actin filaments generates contractile forces in so-called actomyosin fibers, both in muscle as well as most non-muscle cell types. Actin structures are controlled by the Rho family of small GTP-binding proteins such as Rho itself for contractile acto-myosin filaments ("stress fibers"), Rac for lamellipodia and Cdc42 for filopodia.
Intermediate filaments are a part of the cytoskeleton of all animals. These filaments, averaging 10 nanometers in diameter, are more stable (strongly bound) than actin filaments, and heterogeneous constituents of the cytoskeleton. Like actin filaments, they function in the maintenance of cell-shape by bearing tension (microtubules, by contrast, resist compression but can also bear tension during mitosis and during the positioning of the centrosome). Intermediate filaments organize the internal tridimensional structure of the cell, anchoring organelles and serving as structural components of the nuclear lamina. They also participate in some cell-cell and cell-matrix junctions. Nuclear lamina exist in all animals and all tissues. Some animals like the fruit fly do not have any cytoplasmic intermediate filaments. In those animals that express cytoplasmic intermediate filaments, these are tissue specific.
Different intermediate filaments are:
- made of vimentins. Vimentin intermediate filaments are in general present in mesenchymal cells.
- made of keratin. Keratin is present in general in epithelial cells.
- neurofilaments of neural cells.
- made of lamin, giving structural support to the nuclear envelope.
Microtubules are hollow cylinders about 23 nm in diameter (lumen = approximately 15 nm in diameter), most commonly comprising 13 protofilaments that, in turn, are polymers of alpha and beta tubulin. They have a very dynamic behavior, binding GTP for polymerization. They are commonly organized by the centrosome.
In nine triplet sets (star-shaped), they form the centrioles, and in nine doublets oriented about two additional microtubules (wheel-shaped), they form cilia and flagella. The latter formation is commonly referred to as a "9+2" arrangement, wherein each doublet is connected to another by the protein dynein. As both flagella and cilia are structural components of the cell, and are maintained by microtubules, they can be considered part of the cytoskeleton.
They play key roles in:
- intracellular transport (associated with dyneins and kinesins, they transport organelles like mitochondria or vesicles).
- the axoneme of cilia and flagella.
- the mitotic spindle.
- synthesis of the cell wall in plants.
|Cytoskeleton type||Diameter (nm)||Structure||Subunit examples|
|Intermediate filaments||10||two anti-parallel helices/dimers, forming tetramers|
|Microtubules||23||protofilaments, in turn consisting of tubulin subunits in complex with stathmin||α- and β-tubulin|
Septins are a group of highly conserved GTP binding proteins found in eukaryotes. Different septins form protein complexes with each other. These can assemble to filaments and rings. Therefore, septins can be considered part of the cytoskeleton. The function of septins in cells include serving as a localized attachment site for other proteins, and preventing the diffusion of certain molecules from one cell compartment to another. In yeast cells, they build scaffolding to provide structural support during cell division and compartmentalize parts of the cell. Recent research in human cells suggests that septins build cages around bacterial pathogens, immobilizing the harmful microbes and preventing them from invading other cells.
In budding yeast (an important model organism), actin forms cortical patches, actin cables, and a cytokinetic ring and the cap. Cortical patches are discrete actin bodies on the membrane and are important for endocytosis, especially the recycling of glucan synthase which is important for cell wall synthesis. Actin cables are bundles of actin filaments and are involved in the transport of vesicles towards the cap (which contains a number of different proteins to polarize cell growth) and in the positioning of mitochondria. The cytokinetic ring forms and constricts around the site of cell division.
The cytoskeleton was once thought to be a feature only of eukaryotic cells, but homologues to all the major proteins of the eukaryotic cytoskeleton have been found in prokaryotes. Although the evolutionary relationships are so distant that they are not obvious from protein sequence comparisons alone, the similarity of their three-dimensional structures and similar functions in maintaining cell shape and polarity provides strong evidence that the eukaryotic and prokaryotic cytoskeletons are truly homologous. However, some structures in the bacterial cytoskeleton may have yet to be identified.
FtsZ was the first protein of the prokaryotic cytoskeleton to be identified. Like tubulin, FtsZ forms filaments in the presence of guanosine triphosphate (GTP), but these filaments do not group into tubules. During cell division, FtsZ is the first protein to move to the division site, and is essential for recruiting other proteins that synthesize the new cell wall between the dividing cells.
MreB and ParM
Prokaryotic actin-like proteins, such as MreB, are involved in the maintenance of cell shape. All non-spherical bacteria have genes encoding actin-like proteins, and these proteins form a helical network beneath the cell membrane that guides the proteins involved in cell wall biosynthesis.
Some plasmids encode a partitioning system that involves an actin-like protein ParM. Filaments of ParM exhibit dynamic instability, and may partition plasmid DNA into the dividing daughter cells by a mechanism analogous to that used by microtubules during eukaryotic mitosis.
The bacterium Caulobacter crescentus contains a third protein, crescentin, that is related to the intermediate filaments of eukaryotic cells. Crescentin is also involved in maintaining cell shape, such as helical and vibrioid forms of bacteria, but the mechanism by which it does this is currently unclear.
Common features and differences between prokaryotes and eukaryotes
By definition, the cytoskeleton is composed of proteins that can form longitudinal arrays (fibres) in all organisms. These filament forming proteins have been classified into 4 classes. Tubulin-like, actin-like, Walker A cytoskeletal ATPases (WACA-proteins), and intermediate filaments. 
Tubulin-like proteins are tubulin in eukaryotes and FtsZ, TubZ, RepX in prokaryotes. Actin-like proteins are actin in eukaryotes and MreB, FtsA in prokaryotes. An example of a WACA-proteins, which are mostly found in prokaryotes, is MinD. Examples for intermediate filaments, which have almost exclusively been found in animals (i.e. eukaryotes) are the lamins, keratins, vimentin, neurofilaments, desmin. 
Although tubulin-like proteins share some amino acid sequence similarity, their similarity in protein-fold and the similarity in the GTP binding site is more striking. The same holds true for the actin-like proteins and their structure and ATP binding domain. 
Cytoskeletal proteins are usually associated with cell shape, DNA segregation and cell division in prokaryotes and eukaryotes. Which proteins fulfill which task is very different. For example DNA segregation in all eukaryotes happens through use of tubulin, but in prokaryotes either WACA proteins, actin-like or tubulin-like proteins can be used. Cell division is mediated in eukaryotes by actin, but in prokaryotes usually by tubulin-like (often FtsZ-ring) proteins and sometimes (Crenarchaeota) ESCRT-III, which in eukaryotes still has a role in the last step of division. 
In 1903, Nikolai K Koltsov proposed that the shape of cells was determined by a network of tubules that he termed the cytoskeleton. The concept of a protein mosaic that dynamically coordinated cytoplasmic biochemistry was proposed by Rudolph Peters in 1929  while the term (cytosquelette, in French) was first introduced by French embryologist Paul Wintrebert in 1931.
A fourth eukaryotic cytoskeletal element, microtrabeculae, was proposed by Keith Porter based on images obtained from high-voltage electron microscopy of whole cells in the 1970s. The images showed short, filamentous structures of unknown molecular composition associated with known cytoplasmic structures. Porter proposed that this microtrabecular structure represented a novel filamentous network distinct from microtubules, filamentous actin, or intermediate filaments. It is now generally accepted that microtrabeculae are nothing more than an artifact of certain types of fixation treatment, although the complexity of the cell's cytoskeleton is not yet fully understood.
- Alberts, Bruce; et al. (2008). Molecular Biology of the Cell (5th ed.). New York: Garland Science. ISBN 978-0-8153-4105-5.
- Wickstead B, Gull K (2011). "The evolution of the cytoskeleton". Journal of Cell Biology 194 (4): 513–525. doi:10.1083/jcb.201102065. PMC 3160578. PMID 21859859.
- Fletcher DA, Mullins D (2010). "Cell mechanics and the cytoskeleton". Nature 463 (7280): 485–92. doi:10.1038/nature08908. PMC 2851742. PMID 20110992.
- Herrmann H, Bär H, Kreplak L, Strelkov SV, Aebi U (July 2007). "Intermediate filaments: from cell architecture to nanomechanics". Nature Review Molecular Cell Biology 8 (7 pages=562-573): 562–73. doi:10.1038/nrm2197. PMID 17551517.
- Geli MI, Riezman H (1998). "Endocytic internalization in yeast and animal cells: similar and different". Journal of Cell Science 111: 1031–1037.
- Minton AP (October 1992). "Confinement as a determinant of macromolecular structure and reactivity". Biophys. J. 63 (4): 1090–100. Bibcode:1992BpJ....63.1090M. doi:10.1016/S0006-3495(92)81663-6. PMC 1262248. PMID 1420928.
- Doherty GJ and McMahon HT (2008). "Mediation, Modulation and Consequences of Membrane-Cytoskeleton Interactions". Annual Review of Biophysics 37: 65–95. doi:10.1146/annurev.biophys.37.032807.125912. PMID 18573073.
- Unless else specified in boxes, then ref is:Walter F., PhD. Boron (2003). Medical Physiology: A Cellular And Molecular Approaoch. Elsevier/Saunders. p. 1300. ISBN 1-4160-2328-3. Page 25
- Fuchs E, Cleveland DW (January 1998). "A structural scaffolding of intermediate filaments in health and disease". Science 279 (5350): 514–9. Bibcode:1998Sci...279..514F. doi:10.1126/science.279.5350.514. PMID 9438837.
- Steinmetz MO (2007). "Structure and thermodynamics of the tubulin-stathmin interaction.". J Struct Biol 158 (2): 137–47. doi:10.1016/j.jsb.2006.07.018. PMID 17029844.
- Mostowy S, Cossart P (2012). "Septins: the fourth component of the cytoskeleton". Nature Reviews Molecular Cell Biology 13 (3): 183–194. doi:10.1038/nrm3284. PMID 22314400.
- Mascarelli A (December 2011). "Septin proteins take bacterial prisoners: A cellular defence against microbial pathogens holds therapeutic potential". Nature. doi:10.1038/nature.2011.9540.
- Pruyne D, Bretscher A (2000). "Polarization of cell growth in yeast II. The role of the cortical actin cytoskeleton". Journal of Cell Science 113: 571–585. PMID 10652251.
- Shih YL, Rothfield L (2006). "The bacterial cytoskeleton". Microbiol. Mol. Biol. Rev. 70 (3): 729–54. doi:10.1128/MMBR.00017-06. PMC 1594594. PMID 16959967.
- Michie KA, Löwe J (2006). "Dynamic filaments of the bacterial cytoskeleton". Annu. Rev. Biochem. 75: 467–92. doi:10.1146/annurev.biochem.75.103004.142452. PMID 16756499.[dead link]
- Briegel A, Dias DP, Li Z, Jensen RB, Frangakis AS, Jensen GJ (October 2006). "Multiple large filament bundles observed in Caulobacter crescentus by electron cryotomography". Mol. Microbiol. 62 (1): 5–14. doi:10.1111/j.1365-2958.2006.05355.x. PMID 16987173.
- Ausmees N, Kuhn JR, Jacobs-Wagner C (December 2003). "The bacterial cytoskeleton: an intermediate filament-like function in cell shape". Cell 115 (6): 705–13. doi:10.1016/S0092-8674(03)00935-8. PMID 14675535.
- Peters RA. "The Harben Lectures, 1929. Reprinted in: Peters, R. A. (1963) Biochemical lesions and lethal synthesis, p. 216. Pergamon Press, Oxford.".
- Frixione E (June 2000). "Recurring views on the structure and function of the cytoskeleton: a 300-year epic". Cell motility and the cytoskeleton 46 (2): 73–94. doi:10.1002/1097-0169(200006)46:2<73::AID-CM1>3.0.CO;2-0. PMID 10891854.
- Wolosewick JJ, Porter KR (July 1979). "Microtrabecular lattice of the cytoplasmic ground substance. Artifact or reality". J. Cell Biol. 82 (1): 114–39. doi:10.1083/jcb.82.1.114. PMC 2110423. PMID 479294.
- Heuser J (2002). "Whatever happened to the 'microtrabecular concept'?". Biol Cell 94 (9): 561–96. doi:10.1016/S0248-4900(02)00013-8. PMID 12732437.
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