The game begins shortly before the Battle of Thermopylae where the player takes on the role as King Leonidas, fighting through scenes from both movie and book. Armour can be upgraded, as can weapons and stats. The player battles through hordes of Persian warriors including slaves, spearmen, archers, Immortals, Persian champions, two Persian generals, Mardonius and Hydarnes. The action is furthered when the Spartan army forms a phalanx formation. This is used to fight not only even more Persians, but giant beasts such as elephants as well. As the game keeps progressing, Leonidas is able to wield two swords and the ability to change between weapon styles.
The game was met with average to poor reviews, with an average score of 54.4% on GameRankings.com  and 55 out of 100 on Metacritic.com  Despite this, 300: March to Glory is part of the PSP's Greatest Hits collection, suggesting high sales figures.