|Sports fielded||13 (men's: 6; women's: 7)|
|Region||Central United States|
|Commissioner||Tony Stigliano (since 1999)|
The Heartland Conference is a collegiate athletic conference affiliated with the National Collegiate Athletic Association (NCAA)'s Division II level, which was founded in 1999. The majority of members are in Texas, with additional members in Arkansas, Kansas, and Oklahoma. The conference office is located in Waco, Texas.
The conference was formed in 1999 by founding members Drury University, University of the Incarnate Word, Lincoln University, Rockhurst University, St. Edward's University, St. Mary's University and Texas Wesleyan University. Oklahoma Panhandle State University and Dallas Baptist University joined in 2002. Founding members Drury and Rockhurst left the Heartland Conference to join the Great Lakes Valley Conference (GLVC) in 2005. Western New Mexico University and Montana State University - Billings joined in 2005. However, WNMU re-joined the Rocky Mountain Athletic Conference in 2006 and MSUB joined the Great Northwest Athletic Conference in 2007. Newman University, Texas A&M International University and the University of Texas of the Permian Basin joined the conference in 2006, making the transition from NAIA to NCAA Division II. The University of Arkansas - Fort Smith joined the conference in the Fall of 2009 after transitioning from the NJCAA. In the fall of 2010, Lincoln left for the Mid-America Intercollegiate Athletics Association and Incarnate Word left for the Lone Star Conference. In July 2011, McMurry University announced that it had been accepted as candidate for D-II membership and would join the Heartland Conference in the fall of 2012. In February 2012, Oklahoma Christian University announced its intention to seek membership in NCAA Division II. In Spring 2012, Rogers State University, a member of the NAIA Sooner Athletic Conference, applied for membership. The conference confirmed in July 2012 that Oklahoma Christian's teams would play full conference schedules starting in Fall 2012 and that Rogers State and Lubbock Christian University would begin conference play in 2013-14.
|University of Arkansas – Fort Smith||Fort Smith, Arkansas||1928||7,000||Lions||2009|
|Dallas Baptist University||Dallas, Texas||1898||5,500||Patriots||2002|
|Lubbock Christian University||Lubbock, Texas||1957||2,100||Chaparrals||2013|
|Newman University||Wichita, Kansas||1933||2,200||Jets||2006|
|Oklahoma Christian University||Oklahoma City, Oklahoma||1950||2,200||Eagles||2012|
|Oklahoma Panhandle State University||Goodwell, Oklahoma||1909||1,200||Aggies||2002|
|Rogers State University||Claremore, Oklahoma||1909||4,227||Hillcats||2013|
|St. Edward's University||Austin, Texas||1885||5,500||Hilltoppers||1999|
|St. Mary's University||San Antonio, Texas||1852||4,500||Rattlers||1999|
|Texas A&M International University||Laredo, Texas||1969||4,298||Dustdevils||2006|
|University of Texas of the Permian Basin||Odessa, Texas||1973||3,600||Falcons||2006|
- Provisional member in red.
|Drury University||Springfield, Missouri||1873||Panthers||1999||2005||Great Lakes Valley|
|University of the Incarnate Word||San Antonio, Texas||1881||Cardinals||1999||2010||Southland
|Lincoln University (MO)||Jefferson City, Missouri||1866||Blue Tigers||1999||2010||Mid-America|
|McMurry University||Abilene, Texas||1923||War Hawks||2012||2014||American Southwest
|Montana State University Billings||Billings, Montana||1927||Yellowjackets||2005||2007||Great Northwest|
|Rockhurst University||Kansas City, Missouri||1910||Hawks||1999||2005||Great Lakes Valley|
|Texas Wesleyan University||Fort Worth, Texas||1890||Rams||1999||2001||Sooner
|Western New Mexico University||Silver City, New Mexico||1893||Mustangs||2005||2006||Rocky Mountain|
- McMurry — has withdrawn D-II membership bid. 
Full member (all sports) Full member (non-football) Associate member (football-only) Associate member (sport)
The Heartland Conference sponsors 13 sports, seven for women and six for men.
Dallas Baptist's baseball team currently competes in D-I as a baseball affiliate of the Missouri Valley Conference; it returned to the MVC baseball conference starting in the 2014 season. OPSU sponsors non-conference football and rodeo squads.
Men's sponsored sports by school
|Oklahoma Panhandle State||4|
|Texas A&M International||5|
Women's sponsored sports by school
|Oklahoma Panhandle State||5|
|Texas A&M International||6|
Other sponsored sports by school
|Oklahoma Panhandle State||IND||IHSA||NIRA|
- ‡ – D-I sport
- PCSC – Pacific Collegiate Swim and Dive Conference
- Texas-Permian Basin – Football begins play in 2016.
|Baseball||St. Mary’s Univ.||2001|
|Softball||St. Mary’s Univ.||2002|
|Men’s Golf (Individual champion)||Jamie Amoretti, St. Mary’s Univ.||2006|
- St. Mary's won NAIA national championships in Softball (1986) and Men's Basketball (1989) prior to joining the Heartland Conference.
- The St. Mary's Men's Golf team were named the Golf Coaches Association of America 2008-2009 Academic National Champions, which St. Mary's treats as a fifth team national championship.
- Dallas Baptist won the 2003 National Christian College Athletic Association Baseball national championship; DBU is a non-baseball member of the Heartland Conference.
- - About the Heartland Conference
- University of Arkansas at Fort Smith Accepts Invitation to Join Heartland Conference - arkansasbusiness.com - March 2, 2009
- Lincoln returns to MIAA - St. Joseph News-Press - February 2, 2009
- LSC to add Incarnate Word in 2010 - www.lonestarconference.org - January 20, 2009
- NCAA accepts McMurry's application to transition to Division II - www.mcmurrysports.com - July 12, 2011
- King, Kevin (February 7, 2012). KTUL TV http://www.ktul.com/story/16692399/oklahoma-christian-to-seek-ncaa-division-ii-membership. Retrieved February 7, 2012. Missing or empty
- Adame, Tony (June 23, 2012). Wichita Eagle http://www.kansas.com/2012/06/23/2384632/neaman-athletics-continues-to.html. Retrieved June 26, 2012. Missing or empty
- http://www.stmarytx.edu/athletics/index.php?site=historyTimeline Rattler Athletics Timeline